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ARID3B Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596] - Staining in human testis and pancreas tissues using anti-ARID3B antibody. Corresponding ARID3B RNA-seq data are presented for ...read more
Western Blot: ARID3B Antibody [NBP2-33596] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). ...read more
Immunocytochemistry/ Immunofluorescence: ARID3B Antibody [NBP2-33596] - Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596] - Staining of human testis shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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ARID3B Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GSSSGTNTSSPRISPATTLRKGDGAPVTTVPVPNRLAVPVTLASQQAGTRTAALEQLR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARID3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ARID3B Protein (NBP2-33596PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ARID3B Antibody

  • ARID domain-containing protein 3B
  • AT rich interactive domain 3B (BRIGHT- like)
  • AT rich interactive domain 3B (BRIGHT-like)
  • AT-rich interactive domain-containing protein 3B
  • BDP
  • bright and dead ringer protein
  • DRIL2

Background

ARID3B encodes a member of the ARID (AT-rich interaction domain) family of DNA-binding proteins. The encoded protein is homologous with two proteins that bind to the retinoblastoma gene product, and also with the mouse Bright and Drosophila dead ringer proteins. A pseudogene on chromosome 1p31 exists for this gene. Members of the ARID family have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation and possibly in chromatin structure modification.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ARID3B
Uniprot