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![Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596] - Staining in human testis and pancreas tissues using anti-ARID3B antibody. Corresponding ARID3B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ARID3B-Antibody-Immunohistochemistry-Paraffin-NBP2-33596-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596] - Staining in human testis and pancreas tissues using anti-ARID3B antibody. Corresponding ARID3B RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: ARID3B Antibody [NBP2-33596] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/ARID3B-Antibody-Western-Blot-NBP2-33596-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ARID3B Antibody [NBP2-33596] - Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/ARID3B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33596-img0007.jpg)
![Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/ARID3B-Antibody-Immunohistochemistry-Paraffin-NBP2-33596-img0008.jpg)
![Immunohistochemistry-Paraffin: ARID3B Antibody [NBP2-33596] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/ARID3B-Antibody-Immunohistochemistry-Paraffin-NBP2-33596-img0009.jpg)
![Western Blot: ARID3B Antibody [NBP1-30456] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Affinity purified rabbit anti-ARID3B used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. ARID3B was also immunoprecipitated by rabbit anti-ARID3B antibodies NBP1-30455 and NBP1-30457, which recognize other epitopes.](http://images.novusbio.com/fullsize/ARID3B-Antibody-Western-Blot-NBP1-30456-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ARID3B Antibody [NBP1-30456] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Affinity purified rabbit anti-ARID3B used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. ARID3B was also immunoprecipitated by rabbit anti-ARID3B antibodies NBP1-30455 and NBP1-30457, which recognize other epitopes.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: ARID3B Antibody [NBP1-30456] - FFPE section of human prostate carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/ARID3B-Antibody-Immunohistochemistry-NBP1-30456-img0006.jpg)
![Immunohistochemistry-Paraffin: ARID3B Antibody [NBP1-30456] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).](http://images.novusbio.com/fullsize/ARID3B-Antibody-Immunohistochemistry-Paraffin-NBP1-30456-img0003.jpg)
![Western Blot: ARID3B Antibody [NBP1-30457] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-ARID3B antibody NBP1-30457 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. ARID3B was also immunoprecipitated by rabbit anti-ARID3B antibodies NBP1-30455 andNBP1-30456, which recognize upstream epitopes.](http://images.novusbio.com/fullsize/ARID3B-Antibody-Western-Blot-NBP1-30457-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ARID3B Antibody [NBP1-30457] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-ARID3B antibody NBP1-30457 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. ARID3B was also immunoprecipitated by rabbit anti-ARID3B antibodies NBP1-30455 andNBP1-30456, which recognize upstream epitopes.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ARID3B Antibody [NBP1-30457] - Human ovarian carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).](http://images.novusbio.com/fullsize/ARID3B-Antibody-Immunohistochemistry-Paraffin-NBP1-30457-img0002.jpg)
![SDS-Page: Recombinant Human ARID3B Protein [H00010620-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00010620-P01-1.jpg "SDS-Page: Recombinant Human ARID3B Protein [H00010620-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunoprecipitation: ARID3B Antibody [NBP1-30455] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-ARID3B antibodyNBP1-30455 used for IP at 10 mcg/mg lysate. ARID3B was also immunoprecipitated by rabbit anti-ARID3B antibodies NBP1-30456 and NBP1-30457, which recognize downstream epitopes.](http://images.novusbio.com/fullsize/ARID3B-Antibody-Immunoprecipitation-NBP1-30455-img0002.jpg "Immunoprecipitation: ARID3B Antibody [NBP1-30455] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-ARID3B antibodyNBP1-30455 used for IP at 10 mcg/mg lysate. ARID3B was also immunoprecipitated by rabbit anti-ARID3B antibodies NBP1-30456 and NBP1-30457, which recognize downstream epitopes.")
![Western Blot: ARID3B Antibody [NBP2-98690] - Anti-ARID3B rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/ARID3B-Antibody-Western-Blot-NBP2-98690-img0001.jpg "Western Blot: ARID3B Antibody [NBP2-98690] - Anti-ARID3B rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa (We are unsure as to the identity of these extra bands).")
![Western Blot: ARID3B Antibody (008) [NBP2-89222] - Lane A: 293T Whole Cell Lysate Lane B: K562 Whole Cell LysateLane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:61 kDa Observed band size:68 kDa](http://images.novusbio.com/fullsize/ARID3B-Antibody-008-Western-Blot-NBP2-89222-img0001.jpg "Western Blot: ARID3B Antibody (008) [NBP2-89222] - Lane A: 293T Whole Cell Lysate Lane B: K562 Whole Cell LysateLane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:61 kDa Observed band size:68 kDa")