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AMPK alpha 2 Antibody (5B4G1) - BSA Free

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Western Blot: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis using PRKAA2 mAb against human PRKAA2 (AA: 453-552) recombinant protein. (Expected MW is 36.7 kDa)
Western Blot: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis using PRKAA2 mAb against human PRKAA2 (AA: 453-552) recombinant protein. (Expected MW is 36.7 kDa)
Immunohistochemistry: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis of paraffin-embedded rectum cancer tissues using PRKAA2 mouse mAb with DAB staining.
Western Blot: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis using PRKAA2 mAb against HEK293 (1) and PRKAA2 (AA: 453-552)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Analysis of paraffin-embedded esophageal cancer tissues using PRKAA2 mouse mAb with DAB staining.
ELISA: AMPK alpha 2 Antibody (5B4G1) [NBP2-61853] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
5B4G1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-61853-0.025ml
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AMPK alpha 2 Antibody (5B4G1) - BSA Free Summary

Immunogen
Purified recombinant fragment of human AMPK alpha 2 (AA: 453-552) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKAA2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:100-1:2000
Theoretical MW
62.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for AMPK alpha 2 Antibody (5B4G1) - BSA Free

  • 5'-AMP-activated protein kinase catalytic subunit alpha-2
  • AMPK alpha 2
  • AMPK subunit alpha-2
  • AMPK2
  • AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain
  • AMPK-alpha-2 chain
  • EC 2.7.11
  • EC 2.7.11.1
  • PRKAA
  • PRKAA2
  • protein kinase, AMP-activated, alpha 2 catalytic subunit

Background

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRKAA2