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Adiponectin/Acrp30 Antibody [Unconjugated]

Images

 
Adiponectin/Acrp30 was detected in immersion fixed ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody ...read more
Adiponectin/Acrp30 was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at ...read more
Chronic administration of compound 21 increases the abundance of adiponectin and UCP‐1 in adipose tissue. (A) and (C) Abundance of adiponectin and UCP‐1 in adipose tissue as determined by western blotting. (B) and ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Adiponectin/Acrp30 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Adiponectin/Acrp30
Glu18-Asn247
Accession # Q60994
Specificity
Detects mouse Adiponectin/Acrp30 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ADIPOQ
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF1119 in the following applications:

Publications
Read Publications using
AF1119 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Adiponectin/Acrp30 Antibody [Unconjugated]

  • ACDC
  • Acrp30
  • ACRP30ADPN
  • adipocyte, C1Q and collagen domain containing
  • Adiponectin
  • adiponectin, C1Q and collagen domain containing
  • AdipoQ
  • ADIPQTL1
  • ApM1
  • apM-1
  • APM1APM-1
  • C1q and collagen domain-containing protein
  • GBP28
  • GBP28apM1
  • Gelatin-binding protein

Background

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity, and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature mouse adiponectin consists of a 66 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-10). Mature mouse adiponectin shares 83% and 91% amino acid (aa) sequence identity with human and rat adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (11-13). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (14). The various multimeric forms of adiponectin exhibit distinct tissue specific and gender specific profiles and activities (13, 15).

  1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
  2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
  3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
  4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
  5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
  6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10308.
  7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
  8. Scherer, P.E. et al. (1995) J. Biol. Chem. 270:26746.
  9. Hu, E. et al. (1996) J. Biol. Chem. 271:10697.
  10. Waki, H. et al. (2005) Endocrinology 146:790.
  11. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
  12. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
  13. Wang, Y. et al. (2008) Biochem. J. 409:623.
  14. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
  15. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Adiponectin/Acrp30 Antibody (AF1119)(33)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ICC
(1)
IHC
(5)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(13)
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Human
(3)
Mouse
(18)
Transgenic Mouse
(1)
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Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using AF1119 Applications Species
Tee, JH;Vijayakumar, U;Shanmugasundaram, M;Lam, TYW;Liao, W;Yang, Y;Wong, WSF;Ge, R; Isthmin-1 attenuates allergic Asthma by stimulating adiponectin expression and alveolar macrophage efferocytosis in mice Respiratory research 2023-11-06 [PMID: 37932719] (Western Blot, Mouse, Human, Transgenic Mouse) Western Blot Mouse, Human, Transgenic Mouse
Keita Fukuoka, Ryohei Mineo, Shunbun Kita, Shiro Fukuda, Tomonori Okita, Emi Kawada-Horitani et al. ER stress decreases exosome production through adiponectin/T-cadherin-dependent and -independent pathways Journal of Biological Chemistry 9/1/2023 [PMID: 37524131]
Tomonori Okita, Shunbun Kita, Shiro Fukuda, Keita Fukuoka, Emi Kawada-Horitani, Masahito Iioka et al. Soluble T-cadherin promotes pancreatic beta -cell proliferation by upregulating Notch signaling iScience 11/18/2022 [PMID: 36439986]
J You, L Sun, J Wang, F Sun, W Wang, D Wang, X Fan, D Liu, Z Xu, C Qiu, J Chen, H Yan, B Liu Role of Adiponectin-Notch pathway in cognitive dysfunction associated with depression and in the therapeutic effect of physical exercise Aging Cell, 2021-05-30;0(0):e13387. 2021-05-30 [PMID: 34053165] (Western Blot, Mouse) Western Blot Mouse
Hua Guan, Huiyuan Zheng, Jin Zhang, Aoqi Xiang, Yongxin Li, Huadong Zheng et al. Secreted frizzled‑related protein 4 promotes brown adipocyte differentiation Experimental and Therapeutic Medicine 4/15/2021 [PMID: 33968168]
Y Nakamura, S Kita, Y Tanaka, S Fukuda, Y Obata, T Okita, H Nishida, Y Takahashi, Y Kawachi, Y Tsugawa-Sh, Y Fujishima, H Nishizawa, Y Takakura, S Miyagawa, Y Sawa, N Maeda, I Shimomura Adiponectin Stimulates Exosome Release to Enhance Mesenchymal Stem-Cell-Driven Therapy of Heart Failure in Mice Mol. Ther., 2020-07-10;0(0):. 2020-07-10 [PMID: 32652045] (Western Blot, Human) Western Blot Human
Jayagopi Surendar, Stefan J. Frohberger, Indulekha Karunakaran, Vanessa Schmitt, Wiebke Stamminger, Anna-Lena Neumann et al. Adiponectin Limits IFN-gamma and IL-17 Producing CD4 T Cells in Obesity by Restraining Cell Intrinsic Glycolysis Frontiers in Immunology 10/29/2019 [PMID: 31736971]
Shunbun Kita, Shiro Fukuda, Norikazu Maeda, Iichiro Shimomura Native adiponectin in serum binds to mammalian cells expressing T-cadherin, but not AdipoRs or calreticulin eLife 10/24/2019 [PMID: 31647413]
Diego T. Quiroga, Johanna G. Miquet, Lorena Gonzalez, Ana I. Sotelo, Marina C. Muñoz, Pedro M. Geraldes et al. Mice lacking angiotensin type 2 receptor exhibit a sex-specific attenuation of insulin sensitivity Molecular and Cellular Endocrinology 12/1/2019 [PMID: 31539597]
DT Quiroga, MC Muñoz, C Gil, M Pffeifer, JE Toblli, UM Steckeling, JF Giani, FP Dominici Chronic administration of the angiotensin type 2 receptor agonist C21 improves insulin sensitivity in C57BL/6 mice Physiol Rep, 2018-08-01;6(16):e13824. 2018-08-01 [PMID: 30156060] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
Show All 33 Publications.

Review for Adiponectin/Acrp30 Antibody (AF1119) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1119:
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WB
(1)
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Mouse
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
WB Mouse 01/05/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23824191
SpeciesMouse

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Verified Customer
01/05/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ADIPOQ
Uniprot