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Acetyl CoA synthetase Antibody

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Immunocytochemistry/ Immunofluorescence: Acetyl CoA synthetase Antibody [NBP3-38275] - Immunofluorescence analysis of NIH-3T3 cells using Acetyl CoA synthetase Rabbit pAb at dilution of 100 (40x lens). Secondary ...read more
Immunocytochemistry/ Immunofluorescence: Acetyl CoA synthetase Antibody [NBP3-38275] - Confocal immunofluorescence analysis of NIH-3T3 cells using Acetyl CoA synthetase Rabbit pAb at dilution of 1:200. Blue: DAPI for ...read more
Western Blot: Acetyl CoA synthetase Antibody [NBP3-38275] - Western blot analysis of various lysates using Acetyl CoA synthetase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Acetyl CoA synthetase Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 462-701 of human Acetyl CoA synthetase (NP_061147.1).

Sequence:
VDTFWQTETGGHMLTPLPGATPMKPGSATFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHERFETTYFKKFPGYYVTGDGCQRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPKLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDMSTVADPSVISHLFSHRCLTIQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACSS2
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
Theoretical MW
79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for Acetyl CoA synthetase Antibody

  • ACAS2
  • AceCS
  • acetate thiokinase
  • acetate-CoA ligase
  • Acetate--CoA ligase
  • Acetyl-CoA synthetase
  • acetyl-Coenzyme A synthetase 2 (ADP forming)
  • acetyl-coenzyme A synthetase, cytoplasmic
  • ACSA
  • ACSACECS
  • Acyl-activating enzyme
  • acyl-CoA synthetase short-chain family member 2EC 6.2.1.1
  • cytoplasmic acetyl-coenzyme A synthetase
  • dJ1161H23.1
  • DKFZp762G026
  • EC 6.2.1

Background

Acetyl CoA synthetase encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ACSS2