ACSL1 Antibody

Long-chain-fatty-acid--CoA ligase 1, or ACSL1, is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. These proteins play an important role in lipid biosynthesis and fatty acid degradation. Like other isozymes of this family, ALCS1 converts free long-chain fatty acids into fatty acyl-CoA esters. ACSL1 preferentially uses palmitoleate, oleate and linoleate, and altered expression of ACSL1 is thought to increase accumulation of triglycerides in the liver.

ALCS1 is strongly expressed in the heart, kindey, liver, skeletal muscle, and erythroid cells, and to a lesser extent in lung, brain, placenta and pancreas. This protein is predominantly expressed during the early stages of erythroid development, and expression is weak in reticulocytes and young erythrocytes.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Images	Ratings	Applications	Species	Date	Details
Enlarge	3 reviewed by: Verified Customer	IHC-Fr	Rat	12/22/2017	View Summary Application Immunohistochemistry-Frozen Sample Tested brain tissue (hippocampus) Species Rat Lot A40997 Comments Comments Name: S100A10 antibody Catalog #: NBP1-89370 Lot Number: A40997 Antibody Storage Conditions:-20℃ Application Tested: IHC-Fr Staining Expectations: co-labell A2 astrocytes with gfap Observation & Concerns: The background is very deep and the A2 astrocyte can’t be stained. Test Sample Information: Species & Treatments: SD rats & infrasound models Tissue: brain tissue (hippocampus) Fixative Composition: 4% paraformaldehyde solution Fixation Time & Temperature: 6 h or longer Tissue Processing: antigen retrieval, EDTA pH9.0, 95C, 15min Blocking Procedure: Blocking Solution: 3%BSA/5%BSA Time & Temperature: 0.5 or 1 h & room temperature Blocking Time: Click here to enter text. Primary Antibody Dilution: 1:25; 1:50; 1:100; 1:200; 1:500 Diluent Buffer: 3%BSA，0.3%Triton Time & Temperature: 12 h & 4℃ Washing Conditions: Wash Buffer Composition: 1 * PBS Method (Immersion, Rinsing etc.): Immersion Times/Washing: 3 * 10 min Repetitions: 3 times Secondary Antibody Manufacturer and Catalog #: CWBIO & CW0114S Secondary description: anti-rabbit secondary antibody is available, see section controls and additional images. Dilution: 1:200 Diluent Buffer: 1 * PBS(hoechest, PH=7.4) Incubation Time & Temperature: 2 h & room temperature Post-Secondary Washing: the same as post-primary washing Detection Method: Detection: DM4000 Controls: Positive Control: the anti-rabbit secondary antibody is available Negative Control: the production can not co-labell A2 ast

Array NBP1-89270

• ACSL1 Antibodies
• ACSL1 Proteins