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MITF Antibody

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MITF was detected in paraffin-embedded sections of human skin using Goat Anti-Human MITF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5769) at 10 µg/mL overnight at 4 °C. Before incubation with ...read more
Western blot shows lysates of Bowes human melanoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MITF Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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MITF Antibody Summary

Immunogen
E. coli-derived recombinant human MITF
Val119-Lys289
Accession # O75030
Specificity
Detects human MITF in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MITF
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5769 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MITF Antibody

  • bHLHe32
  • bHLHe32MI
  • Class E basic helix-loop-helix protein 32
  • MI
  • microphthalmia-associated transcription factor
  • MITF
  • Waardenburg syndrome, type 2A
  • WS2A

Background

MITF (Microphthalmia-associated transcription factor) is a member of the MiT/TFE family of molecules. Although it has a predicted MW of 58 kDa, it runs anomalously in SDS-PAGE at 57‑66 kDa. MITF-A1 is found in melanocytes and RPEs where it regulates melanin synthesis via tyrosinase and TRP-1 gene expression. Human MITF-A1 is 526 amino acids (aa) in length. It contains a bHLH DNA binding region (aa 309‑369) and a Leu-zipper domain (aa 374‑395). There are three phosphorylation sites at Ser54/405/414, and two SUMOylation sites at Arg289/423. MITF-A1 acts as either a homodimer, or heterodimer with TFE3, TFEB or TFEC. Multiple splice variants exist, with each variant expressing one of two isoforms that are defined by the presence (#1), or absence (#2), of aa 294‑299. One variant has a deletion of aa 139‑194, a second has an 11 aa substitution for aa 1‑118, and three others show variable substitutions over aa 1‑35. Over aa 119‑289, human MITF-A1 shares 96% aa identity with mouse MITF-A1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MITF Antibody (AF5769)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, ICC/IF, IHC-Fr.


Filter By Application
ICC
(1)
ICC/IF
(1)
IHC-Fr
(1)
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Filter By Species
Human
(2)
Mouse
(1)
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Showing Publications 1 - 9 of 9.
Publications using AF5769 Applications Species
N Comandante, DG Baumann, M Fallahi-Si AP-1 transcription factor network explains diverse patterns of cellular plasticity in melanoma cells Cell Reports, 2022-08-02;40(5):111147. 2022-08-02 [PMID: 35926467] (ICC/IF, Human) ICC/IF Human
Justine M. Renauld, Vibhuti Khan, Martín L. Basch Intermediate Cells of Dual Embryonic Origin Follow a Basal to Apical Gradient of Ingression Into the Lateral Wall of the Cochlea Frontiers in Cell and Developmental Biology 3/8/2022 [PMID: 35372344]
Sophie Colombo, Valérie Petit, Roselyne Y. Wagner, Delphine Champeval, Ichiro Yajima, Franck Gesbert et al. Stabilization of beta -catenin promotes melanocyte specification at the expense of the Schwann cell lineage Development 1/15/2022 [PMID: 34878101]
Marketa Kaucka, Bara Szarowska, Michaela Kavkova, Maria Eleni Kastriti, Polina Kameneva, Inga Schmidt et al. Nerve-associated Schwann cell precursors contribute extracutaneous melanocytes to the heart, inner ear, supraorbital locations and brain meninges Cellular and Molecular Life Sciences 8/1/2021 [PMID: 34274976]
MS Prasad, RM Charney, LJ Patel, MI García-Cas Distinct molecular profile and restricted stem cell potential defines the prospective human cranial neural crest from embryonic stem cell state Stem Cell Research, 2020-11-11;49(0):102086. 2020-11-11 [PMID: 33370869] (ICC, Human) ICC Human
Gustavo A. Gomez, Maneeshi S. Prasad, Man Wong, Rebekah M. Charney, Patrick B. Shelar, Nabjot Sandhu et al. WNT/ beta -catenin modulates the axial identity of embryonic stem cell-derived human neural crest Development 1/7/2019 [PMID: 31399472]
Ruth Halaban, Antonella Bacchiocchi, Robert Straub, Jian Cao, Mario Sznol, Deepak Narayan et al. A novel anti-melanoma SRC-family kinase inhibitor Oncotarget 3/19/2019 [PMID: 31040916]
Gustavo A. Gomez, Maneeshi S. Prasad, Nabjot Sandhu, Patrick B. Shelar, Alan W. Leung, Martín I. García-Castro Human neural crest induction by temporal modulation of WNT activation Developmental Biology 5/1/2019 [PMID: 30826399]
Adameyko I, Lallemend F, Furlan A, Zinin N, Aranda S, Kitambi SS, Blanchart A, Favaro R, Nicolis S, Lubke M, Muller T, Birchmeier C, Suter U, Zaitoun I, Takahashi Y, Ernfors P Sox2 and Mitf cross-regulatory interactions consolidate progenitor and melanocyte lineages in the cranial neural crest. Development, 2012-01-01;139(2):397-410. 2012-01-01 [PMID: 22186729] (IHC-Fr, Mouse) IHC-Fr Mouse

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MITF
Uniprot