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Serpin A5/Protein C Inhibitor Antibody (210326) [Unconjugated]

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HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serpin A5 Monoclonal Antibody (Catalog # MAB1266, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serpin A5 Monoclonal Antibody (Catalog # MAB1266, filled histogram) or isotype control antibody (MAB003, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, CyTOF-ready, Flow
Clone
210326
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1266U
Alexa Fluor 350
IC1266U-100UG
Alexa Fluor 405
IC1266V-100UG
Alexa Fluor 405
FAB1266V
Alexa Fluor 488
IC1266G-100UG
Alexa Fluor 488
FAB1266G
Alexa Fluor 532
FAB1266X
Alexa Fluor 594
IC1266T-100UG
Alexa Fluor 594
FAB1266T
Alexa Fluor 647
IC1266R-100UG
Alexa Fluor 647
FAB1266R
Alexa Fluor 700
IC1266N-100UG
Alexa Fluor 700
FAB1266N
Alexa Fluor 750
FAB1266S
Alexa Fluor 750
IC1266S-100UG
Allophycocyanin
FAB1266A
Biotin
FAB1266B
DyLight 350
FAB1266D
DyLight 405
FAB1266E
DyLight 488
FAB1266K
DyLight 550
FAB1266L
DyLight 594
FAB1266M
DyLight 650
FAB1266W
DyLight 680
FAB1266Y
DyLight 755
FAB1266Z
FITC
FAB1266F
HRP
FAB1266H
Janelia Fluor 549
FAB1266I
Janelia Fluor 646
FAB1266J
PE
FAB1266P
PerCP
FAB1266C
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1266-SP
Carrier Free
IC1266V-100UG
Carrier Free
IC1266U-100UG
Carrier Free
IC1266T-100UG
Carrier Free
IC1266S-100UG
Carrier Free
IC1266R-100UG
Carrier Free
IC1266N-100UG
Carrier Free
IC1266G-100UG
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Serpin A5/Protein C Inhibitor Antibody (210326) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A5/Protein C
His20-Pro406
Accession # P05154
Specificity
Detects human Serpin A5/Protein C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Serpin A1, A3, F2, C1, or recombinant mouse Serpin A5 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SERPINA5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunoprecipitation 25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB1266 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A5/Protein C Inhibitor Antibody (210326) [Unconjugated]

  • Acrosomal serine protease inhibitor
  • antitrypsin), member 5
  • member 5
  • PAI-3
  • PAI3PLANH3
  • PCI
  • PCIplasminogen activator inhibitor III
  • Plasminogen activator inhibitor 3
  • plasminogen activator inhibitor-3
  • PROCIplasma serine protease inhibitor
  • Protein C inhibitor
  • serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase
  • Serpin A5
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)
  • SERPNA5

Background

Serpin A5 is the a member of the Serpin superfamily and inhibits a variety of serine proteases such as protein C, plasminogen activators, thrombin, factor Xa, several kallekreins and acrosin (1). Serpin A5 is synthesized in the liver and secreted in plasma. It is found in numerous steroid-responsive organs and has been detected in saliva, cerebral spinal fluid, amniotic fluid, tears and semen. Because of its protease targets and regulated expression patterns, Serpin A5 has been proposed to play a role in processes such as blood coagulation, fertilization and carcinogenesis (2, 3). Similar to Serpins C1 and D1, its thrombin inhibitory activity is enhanced by heparin.

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Palmier, D. et al. (2002) J. Biol. Chem. 277:40950.
  3. Geiger, M. et al. (1996) Immunopharmacology 32:53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serpin A5/Protein C Inhibitor Antibody (MAB1266)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Co-IP, IHC, Western Blot.


Filter By Application
Co-IP
(1)
IHC
(1)
Western Blot
(2)
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Human
(2)
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Bioinformatics

Gene Symbol SERPINA5
Uniprot