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ACBP Antibody - Azide and BSA Free

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Western Blot: ACBP Antibody [NBP2-92856] - Analysis of extracts of various cell lines, using DBI antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug ...read more
Immunocytochemistry/ Immunofluorescence: ACBP Antibody [NBP2-92856] - Immunofluorescence analysis of U2OS cells using ACBP Rabbit pAb (NBP2-92856) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ACBP Antibody [NBP2-92856] - Immunohistochemistry of paraffin-embedded rat ovary using ACBP Rabbit pAb (NBP2-92856) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval ...read more
Western Blot: ACBP Antibody [NBP2-92856] - Analysis of extracts of various cell lines, using DBI antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug ...read more
Immunocytochemistry/ Immunofluorescence: ACBP Antibody [NBP2-92856] - Immunofluorescence analysis of A-549 cells using ACBP Rabbit pAb (NBP2-92856) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: ACBP Antibody [NBP2-92856] - Immunofluorescence analysis of NIH/3T3 cells using ACBP Rabbit pAb (NBP2-92856) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: ACBP Antibody [NBP2-92856] - Immunofluorescence analysis of PC-12 cells using ACBP Rabbit pAb (NBP2-92856) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ACBP Antibody [NBP2-92856] - Immunohistochemistry of paraffin-embedded rat liver using ACBP Rabbit pAb (NBP2-92856) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

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ACBP Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-104 of human DBI (NP_001171513.1). MWGDLWLLPPASANPGTGTEAEFEKAAEEVRHLKTKPSDEEMLFIYGHYKQATVGDINTERPGMLDFTGKAKWDAWNELKGTSKEDAMKAYINKVEELKKKYGI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DBI
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:500 - 1:1000
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.05% Proclin 300
Purity
Affinity purified

Alternate Names for ACBP Antibody - Azide and BSA Free

  • ACBP
  • acyl-Coenzyme A binding domain containing 1
  • acyl-Coenzyme A bindingprotein)
  • CCK-RP
  • cholecystokinin-releasing peptide, trypsin-sensitive
  • DBI
  • diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein)
  • Diazepam-binding inhibitor
  • Endozepine
  • EP
  • GABA receptor modulator
  • MGC70414

Background

ACBP encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. ACBP is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DBI