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XCL1/Lymphotactin Antibody [Unconjugated]

 (7 Publications)
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Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human XCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse XCL1/Lymphotactin (0.15 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse XCL1/Lymphotactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF486). The ND50 is typically 5-15 µg/mL.
Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human XCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF486
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Catalog# & Formulation Size Price
Carrier Free
AF486-SP
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XCL1/Lymphotactin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse XCL1/Lymphotactin
Specificity
Detects XCL1/Lymphotactin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) GRO alpha , rhGRO beta , rhGRO gamma , and recombinant mouse MCP-5 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
XCL1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF486 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XCL1/Lymphotactin Antibody [Unconjugated]

  • ATAC
  • ATACSmall-inducible cytokine C1
  • C motif chemokine 1
  • chemokine (C motif) ligand 1
  • Cytokine SCM-1
  • LPTNSCM-1-alpha
  • LTNXC chemokine ligand 1
  • Lymphotactin
  • lymphotaxin
  • SCM-1 alpha
  • SCM-1
  • SCM1A
  • SCM-1a
  • SCYC1SCM1
  • single cysteine motif 1a
  • small inducible cytokine subfamily C, member 1 (lymphotactin)
  • XCL1

Background

Mouse lymphotactin (Lptn) and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse.

  1. Kelner, G. et al. (1994) Science 266:1395.
  2. Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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XCL1/Lymphotactin
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AF486
XCL1/Lymphotactin Antibody [Unconjugated]
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Publications for XCL1/Lymphotactin Antibody (AF486)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ELISA Development, Flow Cytometry, Immunohistochemistry, Neutralization.


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ELISA Development
(1)
Flow Cytometry
(2)
Immunohistochemistry
(1)
Neutralization
(1)
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Mouse
(4)
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Showing Publications 1 - 7 of 7.
Publications using AF486 Applications Species
Han, SJ;Stacy, A;Corral, D;Link, VM;De Siqueira, MK;Chi, L;Teijeiro, A;Yong, DS;Perez-Chaparro, PJ;Bouladoux, N;Lim, AI;Enamorado, M;Belkaid, Y;Collins, N; Microbiota configuration determines nutritional immune optimization Proceedings of the National Academy of Sciences of the United States of America 2023-12-05 [PMID: 38011570] (Flow Cytometry, Immunohistochemistry, Mouse) Flow Cytometry, Immunohistochemistry Mouse
Yu-Chen Wang, Yang Cao, Calvin Pan, Zhiqiang Zhou, Lili Yang, Aldons J. Lusis Intestinal cell type-specific communication networks underlie homeostasis and response to Western diet Journal of Experimental Medicine 2023-05-01 [PMID: 36880999]
Ciechanowska A, Rojewska E, Piotrowska A et al. New insights into the analgesic properties of the XCL1/XCR1 and XCL1/ITGA9 axes modulation under neuropathic pain conditions - evidence from animal studies Frontiers in immunology 2022-12-22 [PMID: 36618360]
Jorge Luis Galeano Niño, Sophie V Pageon, Szun S Tay, Feyza Colakoglu, Daryan Kempe, Jack Hywood et al. Cytotoxic T cells swarm by homotypic chemokine signalling eLife 2020-10-13 [PMID: 33046212]
JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control Cell, 2018-02-08;172(5):1022-1037.e14. 2018-02-08 [PMID: 29429633] (Neutralization, Mouse) Neutralization Mouse
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Yamanaka H, Hoyt T, Yang X, Bowen R, Golden S, Crist K, Becker T, Maddaloni M, Pascual DW A parenteral DNA vaccine protects against pneumonic plague. Vaccine, 2010-03-01;28(18):3219-30. 2010-03-01 [PMID: 20197132] (ELISA Development, Mouse) ELISA Development Mouse

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Bioinformatics

Gene Symbol XCL1