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XCR1/CCXCR1 Antibody [Unconjugated]

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Human peripheral blood monocytes were treated for 16 hours with 1 μg/mL LPS then stained with Goat Anti-Human XCR1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF857, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Human peripheral blood monocytes were treated for 16 hours with 1 μg/mL LPS then stained with Goat Anti-Human XCR1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF857, filled histogram) or isotype control ...read more
NS0 mouse myeloma cell line transfected with human XCR-1 and eGFP was stained with and either (A) Goat Anti-Human XCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF857) or (B) Normal Goat IgG Control ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF857
Phycoerythrin
FAB857P-025
Phycoerythrin
FAB857P-100
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB857P-025
Carrier Free
FAB857P-100
Carrier Free
AF857-SP
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XCR1/CCXCR1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human XCR1
Met1-Thr31, Ser89-Lys103, His168-His190, Phe251-Tyr267
Accession # P46094
Specificity
Detects human XCR1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
XCR1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XCR1/CCXCR1 Antibody [Unconjugated]

  • CCXCR1
  • CCXCR1XC chemokine receptor 1
  • chemokine (C motif) receptor 1
  • chemokine XC receptor 1
  • G protein-coupled receptor 5
  • GPR5
  • GPR5chemokine (C motif) XC receptor 1
  • G-protein coupled receptor 5
  • Lymphotactin receptor
  • XCR1

Background

XCR1, also known as GPR5 and lymphotactin/SCM-1 (single cysteine motif 1) receptor, is a 38 kDa member of the G-protein coupled receptor 1 family. It binds XCL1/lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta . In addition, human herpesvirus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XCR1. XCR1 is expressed on neutrophils, CD8+ T cells , NK cells, CD4+ T cells and B cells. Human XCR1 is a 333 amino acid (aa), 7-transmembrane molecule. It contains a 32 aa N-terminus that lacks glycosylation sites and a 42 aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XCR1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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XCR1/CCXCR1 Antibody [Unconjugated]
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Applications: WB, Flow, CyTOF-ready, ICC/IF

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Bioinformatics

Gene Symbol XCR1
Uniprot