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XCL1/Lymphotactin Antibody [Biotin]

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XCL1/Lymphotactin was detected in perfusion fixed frozen sections of mouse thymus using Mouse XCL1/Lymphotactin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF486) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
XCL1/Lymphotactin was detected in perfusion fixed frozen sections of mouse thymus using Mouse XCL1/Lymphotactin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF486) at 15 µg/mL overnight ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF486
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF486
Carrier Free
AF486
Carrier Free
AF486-SP
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XCL1/Lymphotactin Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant mouse XCL1/Lymphotactin
Specificity
Detects mouse XCL1/Lymphotactin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Lymphotactin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
XCL1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse XCL1/Lymphotactin Antibody (Catalog # MAB486)
ELISA Detection: Mouse XCL1/Lymphotactin Biotinylated Antibody (Catalog # BAF486)
Standard: Recombinant Mouse XCL1/Lymphotactin (Catalog # 486-LT)
Readout System
Publications
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BAF486 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XCL1/Lymphotactin Antibody [Biotin]

  • ATAC
  • ATACSmall-inducible cytokine C1
  • C motif chemokine 1
  • chemokine (C motif) ligand 1
  • Cytokine SCM-1
  • LPTNSCM-1-alpha
  • LTNXC chemokine ligand 1
  • Lymphotactin
  • lymphotaxin
  • SCM-1 alpha
  • SCM-1
  • SCM1A
  • SCM-1a
  • SCYC1SCM1
  • single cysteine motif 1a
  • small inducible cytokine subfamily C, member 1 (lymphotactin)
  • XCL1

Background

Mouse lymphotactin (Lptn) and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse.

  1. Kelner, G. et al. (1994) Science 266:1395.
  2. Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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XCL1/Lymphotactin
Antibody [Biotin]
BAF486
XCL1/Lymphotactin Antibody [Biotin]
Species: Mu
Applications: WB, IHC, ELISA(Det)

Publications for XCL1/Lymphotactin Antibody (BAF486)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ELISA Development.


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Bioinformatics

Gene Symbol XCL1