Home » VCAM-1/CD106 » VCAM-1/CD106 Antibodies » VCAM-1/CD106 Antibody (112734) [Janelia Fluor® 549]
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VCAM-1/CD106 Antibody (112734) [Janelia Fluor® 549]

 
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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, Flow, CyTOF-ready, ELISA, ELISA(Cap), ELISA(Det)
Clone
112734
Clonality
Monoclonal
Host
Rat
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB6432U-100UG
Alexa Fluor 405
FAB6432V-100UG
Alexa Fluor 488
FAB6432G-100UG
Alexa Fluor 532
FAB6432X
Alexa Fluor 594
FAB6432T-100UG
Alexa Fluor 647
FAB6432R-100UG
Alexa Fluor 700
FAB6432N
Alexa Fluor 750
FAB6432S-100UG
Allophycocyanin/Cy7
FAB6432APCCY7
Biotin
FAB6432B
CoraFluor 1
FAB6432CL1
DyLight 350
FAB6432D
DyLight 405
FAB6432E
DyLight 488
FAB6432K
DyLight 550
FAB6432L
DyLight 594
FAB6432M
DyLight 650
FAB6432W
DyLight 680
FAB6432Y
DyLight 755
FAB6432Z
FITC
FAB6432F
HRP
FAB6432H
Janelia Fluor 525
FAB6432JF525
Janelia Fluor 585
FAB6432JF585
Janelia Fluor 635
FAB6432JF635
Janelia Fluor 646
FAB6432J
Janelia Fluor 669
FAB6432JF669
PE/Atto594
FAB6432PEATT594
PE/Cy5.5
FAB6432PECY55
PE/Cy7
FAB6432PECY7
Phycoerythrin
FAB6432P
Unconjugated
MAB6432-100
mFluor Violet 450 SE
FAB6432MFV450
mFluor Violet 500 SE
FAB6432MFV500
mFluor Violet 610 SE
FAB6432MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB6432-100
Carrier Free
FAB6432N
Carrier Free
FAB6432P
Carrier Free
MAB6432-SP
Carrier Free
MAB6432-500
Carrier Free
MAB6432-100
Carrier Free
FAB6432V-100UG
Carrier Free
FAB6432U-100UG
Carrier Free
FAB6432T-100UG
Carrier Free
FAB6432S-100UG
Carrier Free
FAB6432R-100UG
Carrier Free
FAB6432G-100UG
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VCAM-1/CD106 Antibody (112734) [Janelia Fluor® 549] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VCAM-1
Phe25-Glu698 (predicted)
Accession # P29533
Specificity
Detects recombinant mouse (rm) VCAM-1 in Western blots and ELISAs. In sandwich immunoassays, no cross-reactivity or interference was observed with recombinant human VCAM-1, rmICAM-1, rmE-Selectin, rmL-Selectin or rmP-Selectin.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
VCAM1
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • ELISA Standard (Matched Pair)
  • Flow Cytometry
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for VCAM-1/CD106 Antibody (112734) [Janelia Fluor® 549]

  • CD106 antigen
  • CD106
  • DKFZp779G2333
  • INCAM-100
  • L1CAM
  • MGC99561
  • vascular cell adhesion molecule 1
  • vascular cell adhesion protein 1
  • V-CAM 1
  • VCAM1
  • VCAM-1

Background

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 ( alpha4/ beta1 integrin) and LPAM-1 ( alpha4/ beta7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAB6432I
VCAM-1/CD106 Antibody (112734) [Janelia Fluor® 549]
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Bioinformatics

Gene Symbol VCAM1