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UCP1 Antibody (Vab12 P4B12*A12) - BSA Free

Images

 
Western Blot: UCP1 Antibody (Vab12 P4B12/A12) [NBP2-50343] - Analysis of Jurkat cell lysates and Anti-UCP1 clone Vab12 P4B12 A12.
Immunohistochemistry: UCP1 Antibody (Vab12 P4B12/A12) [NBP2-50343] - Staining of human breast cancer tissue shows cytoplasmic positivity.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
Vab12 P4B12*A12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

UCP1 Antibody (Vab12 P4B12*A12) - BSA Free Summary

Description
The clone number is listed as Vab12 P4B12*A12 in the scientific literature.
Immunogen
Ovalbumin-conjugated synthetic peptide - (10 amino acids) derived from the middle polypeptide region of human UCP1. The exact sequence is proprietary.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
UCP1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
Application Notes
Positive control(s): WB- Jurkat whole cell lysate or overexpression cell lysate; IHC-formalin-fixed, paraffin-embedded human breast cancer.

The clone number is listed as Vab12 P4B12*A12 in the scientific literature.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for UCP1 Antibody (Vab12 P4B12*A12) - BSA Free

  • SLC25A7
  • SLC25A7mitochondrial brown fat uncoupling protein 1
  • Solute carrier family 25 member 7
  • Thermogenin
  • UCP 1
  • UCP
  • UCP1
  • uncoupling protein 1 (mitochondrial, proton carrier)

Background

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UCP1