Novus Biologicals products are now on bio-techne.com

Sort By:

Products (7)

page of 1

UCP1 Antibodies

We offer UCP1 Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Intracellular Staining by Flow Cytometry, Peptide ELISA, Sandwich ELISA, Simple Western, Western Blot. Each UCP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our UCP1 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the UCP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our UCP1 polyclonal antibodies and browse our UCP1 monoclonal antibody catalog.

Alternate Names for UCP1 Antibodies

anti-UCP1 antibody, anti-SLC25A7mitochondrial brown fat uncoupling protein 1 antibody, anti-Solute carrier family 25 member 7 antibody, anti-thermogenin antibody, anti-UCP 1 antibody, anti-UCP antibody, anti-uncoupling protein 1 (mitochondrial, proton carrier) antibody

7 Results for "ucp1" in Primary Antibodies

MAB6158

UCP1 Antibody (536435) [Unconjugated]

(51 Publications)

100 μg

MAB6158

Western blot shows recombinant human UCP1, recombinant human UCP2, recombinant human UCP3, and recombinant human UCP4 (5 ng/lane). PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Western blot shows lysates of mouse brown adipose tissue and mouse adipose tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UCP1 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

UCP1 was detected in immersion fixed human mesenchymal stem cells undifferentiated (lower panel) or differentiated into adipocytes (upper panel) using Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Simple Western lane view shows lysates of mouse adipose tissue and mouse brown adipose tissue, loaded at 0.5 mg/mL. A specific band was detected for UCP1 at approximately 37 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse UCP1 Monoclonal Antibody (Catalog # MAB6158). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

IRX3/Irx3 expression is induced in browning adipose tissues.(A) Relative mRNA levels of Ucp1 and Irx3 in IWAT from mice under cold stress (4 °C) for one week (n = 6) and at 25 °C (n = 8). (B–C) Relative mRNA levels of Ucp1 and Irx3 in IWAT (B) and BAT (C) from mice subjected to PBS or CL-316,243 for 10 days. For A–C, gene expression was normalized to 36b4. (D–E) Protein levels of Ucp1 and Irx3 (left) and the quantification value of Irx3 relative to Hsp90 (right) in IWAT (D) and BAT (E) in the mice subjected to PBS or CL-316,243 treatment. (F) Representative images of immunohistochemical staining in IWAT from mice subjected to PBS or CL-316,243 treatment (scale bar, 50 μm for the upper and middle panels, and 100 μm for the bottom panel). (G) PET-CT, gross image, HE staining (scale bar, 20 μm), and electron microscopy (scale bar, 5 μm) of the browning WAT from pheochromocytoma patients. (H) Representative images of immunohistochemical staining in oWAT from pheochromocytoma patients (scale bar, 100 μm for the upper panel, and 50 μm for the bottom panel). Data were presented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. The results are representative of at least three independent experiments. IWAT, inguinal white adipose tissue. BAT, brown adipose tissue. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28988979), licensed under a CC-BY license. Not internally tested by R&D Systems.

IRX3/Irx3 expression is induced in browning adipose tissues.(A) Relative mRNA levels of Ucp1 and Irx3 in IWAT from mice under cold stress (4 °C) for one week (n = 6) and at 25 °C (n = 8). (B–C) Relative mRNA levels of Ucp1 and Irx3 in IWAT (B) and BAT (C) from mice subjected to PBS or CL-316,243 for 10 days. For A–C, gene expression was normalized to 36b4. (D–E) Protein levels of Ucp1 and Irx3 (left) and the quantification value of Irx3 relative to Hsp90 (right) in IWAT (D) and BAT (E) in the mice subjected to PBS or CL-316,243 treatment. (F) Representative images of immunohistochemical staining in IWAT from mice subjected to PBS or CL-316,243 treatment (scale bar, 50 μm for the upper and middle panels, and 100 μm for the bottom panel). (G) PET-CT, gross image, HE staining (scale bar, 20 μm), and electron microscopy (scale bar, 5 μm) of the browning WAT from pheochromocytoma patients. (H) Representative images of immunohistochemical staining in oWAT from pheochromocytoma patients (scale bar, 100 μm for the upper panel, and 50 μm for the bottom panel). Data were presented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. The results are representative of at least three independent experiments. IWAT, inguinal white adipose tissue. BAT, brown adipose tissue. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28988979), licensed under a CC-BY license. Not internally tested by R&D Systems.

BMP7 upregulated browning markers in both SC and DN derived differentiated adipocytes. SC and DN preadipocytes were differentiated and treated as in Figure 1. (A) Browning content as estimated by BATLAS Webtool (n = 9); (B) texture sum variance as quantified by laser-scanning cytometry (n = 5); quantification of UCP1 gene expression by (C) RNA-sequencing (n = 9) and (D) RT-qPCR normalized to GAPDH (n = 5). (E) UCP1 protein expression normalized to beta -actin (n = 5); (F) representative UCP1 immunostaining images visualized by confocal microscopy. Nucleus was stained using propidium iodide (PI), BF represents bright field image. Scale bars represent 10 μm. (G) Quantification of UCP1 immunostaining intensity assisted by laser-scanning cytometry (n = 4000 cells of 4 donors). Data expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Statistics: Paired t-test (A,B,G), GLM (C), and one-way ANOVA with Tukey’s post-test (D,E). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34832860), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC6158U-100UG
Alexa Fluor 405	IC6158V-100UG
Alexa Fluor 488	IC6158G-100UG
Alexa Fluor 532	FAB6158X
Alexa Fluor 594	IC6158T-100UG
Alexa Fluor 647	IC6158R-100UG
Alexa Fluor 700	IC6158N-100UG
Alexa Fluor 750	IC6158S-100UG
Allophycocyanin	FAB6158A
Biotin	FAB6158B
CoraFluor 1	FAB6158CL1
DyLight 350	FAB6158D
DyLight 405	FAB6158E
DyLight 488	FAB6158K
DyLight 550	FAB6158L
DyLight 594	FAB6158M
DyLight 650	FAB6158W
DyLight 680	FAB6158Y
DyLight 755	FAB6158Z
FITC	FAB6158F
HRP	FAB6158H
Janelia Fluor 525	FAB6158JF525
Janelia Fluor 549	FAB6158I
Janelia Fluor 585	FAB6158JF585
Janelia Fluor 635	FAB6158JF635
Janelia Fluor 646	FAB6158J
Janelia Fluor 669	FAB6158JF669
PerCP	FAB6158C
Phycoerythrin	IC6158P-100
Phycoerythrin	IC6158P-025
mFluor Violet 450 SE	FAB6158MFV450
mFluor Violet 500 SE	FAB6158MFV500
mFluor Violet 610 SE	FAB6158MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6158-SP
Carrier Free	IC6158P-025
Carrier Free	IC6158U-100UG
Carrier Free	IC6158S-100UG
Carrier Free	IC6158T-100UG
Carrier Free	IC6158N-100UG
Carrier Free	IC6158V-100UG
Carrier Free	IC6158R-100UG
Carrier Free	IC6158G-100UG
Carrier Free	IC6158P-100

compare

(select up to 3 products from this page to compare)

NBP2-20796

UCP1 Antibody

(3 Publications)

0.1 ml

NBP2-20796

Western Blot: UCP1 Antibody [NBP2-20796] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: UCP1 Antibody [NBP2-20796] - Mouse brown adipocyte. UCP1 stained by UCP1 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: UCP1 Antibody [NBP2-20796] - Human colon carcinoma, using UCP1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: UCP1 Antibody [NBP2-20796] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody (NBP2-20796) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NB100-2828

UCP1 Antibody

(6 Publications)

0.1 mg

NB100-2828

Western Blot: UCP1 Antibody [NB100-2828] - (1 ug/mL) staining of Rat Adipose (A) and (0.5 ug/mL) Skeletal Muscle (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Reactivity:

Hu, Mu, Rt, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Ca

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP2-43550

UCP1 Antibody (3111)

(1 Publication)

0.1 ml

NBP2-43550

Western Blot: UCP1 Antibody (3111) [NBP2-43550] - Analysis of Mouse tissue extracts (50 ug) was separated by 12 % SDS-PAGE, and the membrane was blotted with UCP1 antibody at a dilution of 1:1000.

Immunohistochemistry-Paraffin: UCP1 Antibody (3111) [NBP2-43550] - Mouse brown adipocyte. UCP1 stained by UCP1 antibody [3111] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Reactivity:

Mu, Hu

Species Glossary

Reactivity: Mu, Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP2-50343

UCP1 Antibody (Vab12 P4B12*A12) - BSA Free

0.1 ml

NBP2-50343

Western Blot: UCP1 Antibody (Vab12 P4B12/A12) [NBP2-50343] - Analysis of Jurkat cell lysates and Anti-UCP1 clone Vab12 P4B12 A12.

Immunohistochemistry: UCP1 Antibody (Vab12 P4B12/A12) [NBP2-50343] - Staining of human breast cancer tissue shows cytoplasmic positivity.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-50343AF350
Alexa Fluor 405	NBP2-50343AF405
Alexa Fluor 488	NBP2-50343AF488
Alexa Fluor 532	NBP2-50343AF532
Alexa Fluor 594	NBP2-50343AF594
Alexa Fluor 647	NBP2-50343AF647
Alexa Fluor 700	NBP2-50343AF700
Alexa Fluor 750	NBP2-50343AF750
Biotin	NBP2-50343B
CoraFluor 1	NBP2-50343CL1
DyLight 350	NBP2-50343UV
DyLight 405	NBP2-50343V
DyLight 488	NBP2-50343G
DyLight 550	NBP2-50343R
DyLight 594	NBP2-50343DL594
DyLight 650	NBP2-50343C
DyLight 680	NBP2-50343FR
DyLight 755	NBP2-50343IR
FITC	NBP2-50343F
HRP	NBP2-50343H
Janelia Fluor 525	NBP2-50343JF525
Janelia Fluor 549	NBP2-50343JF549
Janelia Fluor 585	NBP2-50343JF585
Janelia Fluor 635	NBP2-50343JF635
Janelia Fluor 646	NBP2-50343JF646
Janelia Fluor 669	NBP2-50343JF669
mFluor Violet 450 SE	NBP2-50343MFV450
mFluor Violet 500 SE	NBP2-50343MFV500
mFluor Violet 610 SE	NBP2-50343MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-50343-0.025ml

compare

(select up to 3 products from this page to compare)

NBP2-43551

UCP1 Antibody (1345)

0.1 ml

NBP2-43551

Immunohistochemistry-Paraffin: UCP1 Antibody (1345) [NBP2-43551] - UCP1 antibody [GT1345] detects UCP1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. UCP1 stained by UCP1 antibody [GT1345] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: UCP1 Antibody (1345) [NBP2-43551] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody [GT1345] (NBP2-43551) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Reactivity:

Mu, Hu

Species Glossary

Reactivity: Mu, Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP3-13004

UCP1 Antibody

100 ul

NBP3-13004

Western Blot: UCP1 Antibody [NBP3-13004] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody (NBP3-13004) diluted at 1:1000.

Immunohistochemistry-Paraffin: UCP1 Antibody [NBP3-13004] - UCP1 antibody detects UCP1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. UCP1 stained by UCP1 antibody (NBP3-13004) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Reactivity:

Mu, Hu, Rt

Species Glossary

Reactivity: Mu, Hu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

page of 1

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

Visit our other sites

©2024 Novus Biologicals, All Rights Reserved.