Western Blot: UCP1 Antibody (3111) [NBP2-43550] - Analysis of Mouse tissue extracts (50 ug) was separated by 12 % SDS-PAGE, and the membrane was blotted with UCP1 antibody at a dilution of 1:1000.
Immunohistochemistry-Paraffin: UCP1 Antibody (3111) [NBP2-43550] - Mouse brown adipocyte. UCP1 stained by UCP1 antibody [3111] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Use in ICC/IF reported in scientific publication (PMID: 32404366). IHC, IHC-P Assay dependent.
Theoretical MW
33 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-43550 in the following applications:
Rat (80%), Rabbit (86%), Sheep (83%), Bovine (83%).
Packaging, Storage & Formulations
Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified
Alternate Names for UCP1 Antibody (3111)
SLC25A7
SLC25A7mitochondrial brown fat uncoupling protein 1
Solute carrier family 25 member 7
Thermogenin
UCP 1
UCP
UCP1
uncoupling protein 1 (mitochondrial, proton carrier)
Background
Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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