![Western Blot: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869] - Analysis of tissue lysates using mouse mAb to UCHL1, NB110-58869, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] pig spinal cord. The single band at 24kDa corresponds to the UCHL1 protein.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-BH7-Western-Blot-NB110-58869-img0006.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869] - Analysis of tissue lysates using mouse mAb to UCHL1, NB110-58869, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] pig spinal cord. The single band at 24kDa corresponds to the UCHL1 protein.")
| Reactivity | Hu, Mu, Rt, Po, Bv, EqSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC, IHC |
| Clone | BH7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant full length human UCHL1 purified from E. coli. [Swiss-Prot# P09936] |
| Localization | Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note: About 30% of total UCHL1 is associated with membranes in brain. |
| Marker | pan-Neuronal Marker |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | UCHL1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This UCHL1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western Blot, where a band can be seen at approximately 24 kDa. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
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| Theoretical MW | 24 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Affinity purified |
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| Publication using NB110-58869 | Applications | Species |
|---|---|---|
| Zhang M, Deng Y, Luo Y et al. Control of BACE1 degradation and APP processing by ubiquitin carboxyl-terminal hydrolase L1. Journal of Neurochemistry. 2011-12-30 [PMID: 22212137] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for UCH-L1/PGP9.5 Antibody (NB110-58869)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: PGP9.5 / UCHL-1 Antibody (BH7) [NB110-58869] - Shows human embryonic kidney cell line 293 stained with mouse monoclonal to UCHL1 (NB110-58869) (green) and rabbit antibody to neurofilament NF-M, (NB300-133), (red). Blue is a DNA stain. Note that some of the Hek293 cells, which perhaps surprisingly have some properties of neuronal lineage cells (4), are beginning to express both UCHL1 and NF-M, both markers of neurons. The majority of cells, however express neither protein. Note that the UCHL1 is cytoplasmic and diffuse while the NF-M is concentrated in cytoplasmic filaments.](http://images.novusbio.com/fullsize/PGP9-5-UCHL-1-Antibody-BH7-Immunocytochemistry-Immunofluorescence-NB110-58869-img0002.jpg "Immunocytochemistry/Immunofluorescence: PGP9.5 / UCHL-1 Antibody (BH7) [NB110-58869] - Shows human embryonic kidney cell line 293 stained with mouse monoclonal to UCHL1 (NB110-58869) (green) and rabbit antibody to neurofilament NF-M, (NB300-133), (red). Blue is a DNA stain. Note that some of the Hek293 cells, which perhaps surprisingly have some properties of neuronal lineage cells (4), are beginning to express both UCHL1 and NF-M, both markers of neurons. The majority of cells, however express neither protein. Note that the UCHL1 is cytoplasmic and diffuse while the NF-M is concentrated in cytoplasmic filaments.")
![Immunohistochemistry Free-Floating: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869] - Analysis of rat hippocampal section stained with mouse mAb to UCHL1, NB110-58869, dilution 1:5,000 in green and costained with rabbit pAb to FOX/NeuN, dilution 1:2,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. The UCHL1 antibody stains the cell body and dendrites of hippocampal neurons, while the FOX3 antibody labels nuclei of the neuronal cells.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-BH7-Immunocytochemistry-Immunofluorescence-NB110-58869-img0005.jpg "Immunohistochemistry Free-Floating: UCH-L1/PGP9.5 Antibody (BH7) [NB110-58869] - Analysis of rat hippocampal section stained with mouse mAb to UCHL1, NB110-58869, dilution 1:5,000 in green and costained with rabbit pAb to FOX/NeuN, dilution 1:2,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. The UCHL1 antibody stains the cell body and dendrites of hippocampal neurons, while the FOX3 antibody labels nuclei of the neuronal cells.")
![Simple Western: PGP9.5 / UCHL-1 Antibody (BH7) [NB110-58869] - Simple Western lane view shows a specific band for PGP9.5/UCHL-1 in 0.02 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PGP9-5-UCHL-1-Antibody-BH7-Simple-Western-NB110-58869-img0004.jpg "Simple Western: PGP9.5 / UCHL-1 Antibody (BH7) [NB110-58869] - Simple Western lane view shows a specific band for PGP9.5/UCHL-1 in 0.02 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")