S100B Antibody

S100B is a zinc- and calcium-binding protein belonging to the S100 protein family within the EF-hand (helix E-loop-helix F) subgroup (1,2). S100B plays a role in normal central nervous system development, is associated with various neurological diseases such as Alzheimer's and Parkinson's, and it serves as a marker for brain injury (1,2). The S100B protein has a homodimeric structure comprised of two 91-amino acid polypeptide monomers each with a theoretical molecular weight of 10.5 kDa (1,2). Furthermore, each monomer contains two EF-hand regions, four helixes, and a hinge region (2). S100B is predominately expressed in astrocytes, oligodendrocytes, and Schwann cells, but also other cell types including adipocytes (1,3). S100B interacts with toll-like receptor 4 (TLR4) and receptor for advanced glycation end products (RAGE), initiating downstream signaling cascades and transcription factors including JNK/JUN, NFkappaB, and p38, leading to caspase and proinflammatory cytokine production (2). Overall outcomes include neuronal apoptosis, neuroinflammation, and neurodegeneration (1,2). S100B is the most commonly studied astroglia and blood brain barrier biomarker in traumatic brain injury (TBI) (3,4). The serum levels of S100B in patients with TBI is indicative of patient outcomes, where high levels correlate with injury severity and mortality (4). S100B is often in used in combination with additional biomarkers such as glial fibrillary acidic protein (GFAP) and ubiquitin c-terminal hydrolase L1 (UCH-L1) (3,4).

References

1. Yardan, T., Erenler, A. K., Baydin, A., Aydin, K., & Cokluk, C. (2011). Usefulness of S100B protein in neurological disorders. JPMA. The Journal of the Pakistan Medical Association, 61(3), 276-281.

2. Langeh, U., & Singh, S. (2021). Targeting S100B Protein as a Surrogate Biomarker and its Role in Various Neurological Disorders. Current neuropharmacology, 19(2), 265-277. https://doi.org/10.2174/1570159X18666200729100427

3. Thelin, E. P., Nelson, D. W., & Bellander, B. M. (2017). A review of the clinical utility of serum S100B protein levels in the assessment of traumatic brain injury. Acta neurochirurgica, 159(2), 209-225. https://doi.org/10.1007/s00701-016-3046-3

4. Wang, K. K., Yang, Z., Zhu, T., Shi, Y., Rubenstein, R., Tyndall, J. A., & Manley, G. T. (2018). An update on diagnostic and prognostic biomarkers for traumatic brain injury. Expert review of molecular diagnostics, 18(2), 165-180. https://doi.org/10.1080/14737159.2018.1428089

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC/IF, IF/IHC, Immunohistochemistry-Paraffin, WB, Western Blot.

Showing Publications 1 - 10 of 14. Show All 14 Publications. Collapse Publications.

Publications using NBP1-87102	Applications	Species
Gonzalez H, Narasipura SD, Shull T et al. An Efficient and Cost-Effective Approach to Generate Functional Human Inducible Pluripotent Stem Cell-Derived Astrocytes Cells 2023-09-26 [PMID: 37830571] (ICC/IF, Human)	ICC/IF	Human
Horowitch B, Lee DY, Ding M et al. Subsets of interferon signaling predict response to immune checkpoint blockade in melanoma patients Clinical cancer research : an official journal of the American Association for Cancer Research 2023-05-26 [PMID: 37233452]
Plebanek, MP;Xue, Y;Nguyen, YV;DeVito, NC;Wang, X;Holtzhausen, A;Beasley, GM;Yarla, N;Thievanthiran, B;Hanks, BA; A SREBF2-dependent gene program drives an immunotolerant dendritic cell population during cancer progression bioRxiv : the preprint server for biology 2023-04-28 [PMID: 37162965] (Immunohistochemistry-Paraffin, Mouse)	Immunohistochemistry-Paraffin	Mouse
Konstantinidis E, Dakhel A, Beretta C, Erlandsson A Long-term effects of amyloid-beta deposits in human iPSC-derived astrocytes Molecular and cellular neurosciences 2023-03-11 [PMID: 36907531] (Western Blot, Human)	Western Blot	Human
Theivanthiran B, Yarla N, Haykal T et al. Tumor-intrinsic NLRP3-HSP70-TLR4 axis drives premetastatic niche development and hyperprogression during anti-PD-1 immunotherapy Science Translational Medicine 2022-11-23 [PMID: 36417489]
Bogdanov L, Shishkova D, Mukhamadiyarov R et al. Excessive Adventitial and Perivascular Vascularisation Correlates with Vascular Inflammation and Intimal Hyperplasia International journal of molecular sciences 2022-10-12 [PMID: 36293013] (IF/IHC, Rat) Details: Dilution used in IHC 1:100	IF/IHC	Rat
ZHOU LN, CUI XJ, SU KX et al. Beneficial reciprocal effects of bone marrow stromal cells and Schwann cells from adult rats in a dynamic co-culture system in vitro without intercellular contact. Mol Med Rep 2015-10-01 [PMID: 26133460] (ICC/IF, Rat, Mouse)	ICC/IF	Rat, Mouse
Hanson MG, Fregoso V, Vrana JD et al. Peripheral nervous system defects in a mouse model for peroxisomal biogenesis disorders. Dev Biol 2014-11-01 [PMID: 25176044] (IF/IHC, Mouse)	IF/IHC	Mouse
Kielar M, Tuy FP, Bizzotto S et al. Mutations in Eml1 lead to ectopic progenitors and neuronal heterotopia in mouse and human. Nat Neurosci 2014-07-01 [PMID: 24859200]
Darcy MJ, Trouche S, Jin SX, Feig LA. Age-Dependent Role for Ras-GRF1 in the Late Stages of Adult Neurogenesis in the Dentate Gyrus. Hippocampus 2014-03-01 [PMID: 24174283] (IF/IHC, Mouse)	IF/IHC	Mouse
Uhlen M, Oksvold P, Algenas C et al. Antibody-based Protein Profiling of the Human Chromosome 21. Mol Cell Proteomics 2012-03-01 [PMID: 22042635]
Chaichana K, Guerrero-Cazares H, Capilla-Gonzalez V et al. Intra-Operatively Obtained Human Tissue: Protocols and Techniques for the Study of Neural Stem Cells. J Neurosci Methods 2009-05-30 [PMID: 19427538]
Hernandez S, Dominko T. Regeneration competence accompanies increased expression of arginine methyltransferase PRMT8 in human adult fibroblasts. Northeast Bioengineering Conference (NEBEC). 2014-01-01 (WB, ICC/IF, Human) Details: PRMT8 antibody used for WB and ICC-IF in experiments involving iRC cell which are acquired via a novel non-viral method implicating exogenous addition of human fibroblast growth factor FGF2 and reduced / 2% O2 concentration. WB showed a band at ~45 kDa and it was compared with other cell types also which included hESCs and cells expressing GST-PRMT8 (Figure 2C). ICC-IF involved 2% paraformaldehyde fixation, permeablization with PBS containing 0.1% Triton X-100, blocking with 5% BSA, primary detection via Alexa-Fluor-488 conjugated secondary antibody (Figure 3).	WB, ICC/IF	Human
Kim HM, Lee SH, Lim J et al. The epidermal growth factor receptor variant type III mutation frequently found in gliomas induces astrogenesis in human cerebral organoids Cell proliferation 2020-12-06 [PMID: 33283409] (Human)		Human
Show All 14 Publications. Collapse Publications.

Array NBP1-87102

• S100B Antibodies
• S100B Antibody Pair
• S100B ELISA Kit
• S100B Lysate
• S100B Proteins
• S100B Proteins and Enzyme
• S100B ELISA