![Western Blot: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of TXNIP on different lysates using anti-TXNIP antibody at 1/1,000 dilution.Positive control: Lane 1: PC-12 Lane 2: Human skeletal muscleLane 3: NIH-3T3 Lane 4: Hela](http://images.novusbio.com/fullsize/TXNIP-Antibody-JM60-35-Western-Blot-NBP2-75692-img0009.jpg "Western Blot: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of TXNIP on different lysates using anti-TXNIP antibody at 1/1,000 dilution.Positive control: Lane 1: PC-12 Lane 2: Human skeletal muscleLane 3: NIH-3T3 Lane 4: Hela")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | JM60-35 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human TXNIP aa 50-99 / 391. (SwissProt: Q9H3M7 Human; SwissProt: Q8BG60 Mouse; SwissProt: Q5M7W1 Rat) |
| Localization | Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | TXNIP |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 44 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Publications using NBP2-75692 | Applications | Species |
|---|---|---|
| Jandova J, Wondrak GT Genomic GLO1 deletion modulates TXNIP expression, glucose metabolism, and redox homeostasis while accelerating human A375 malignant melanoma tumor growth Redox biology 2020-12-17 [PMID: 33360689] (IHC-P, WB) | IHC-P, WB | |
| Chu Y, Zhang Y, Gao G, et al. Chorionic Villous Mesenchymal Stem Cells Derived Exosomes Deliver miR-135b-5p to Trophoblasts, Promoting their Proliferation and Invasion by Targeting TXNIP via beta -Catenin Pathway Research Square 2020-12-28 (WB) | WB | |
| Rolver M, Holland L, Prasad N Et al. Chronic Acidosis Rewires Cancer Cell Metabolism Through PPAR alpha Signaling SSRN Electronic Journal 2021-08-18 [PMID: 36533672] | ||
| Jandova J, Wondrak GT Vemurafenib drives EMT gene expression in BRAFi-resistant BRAFV600E/NRASQ61K melanoma enhancing tumor growth and metastasis in a bioluminescent murine model The Journal of investigative dermatology 2021-10-20 [PMID: 34687745] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TXNIP |
![Immunocytochemistry/Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692] - Staining TXNIP in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JM60-35-Immunocytochemistry-Immunofluorescence-NBP2-75692-img0004.jpg "Immunocytochemistry/Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692] - Staining TXNIP in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-TXNIP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JM60-35-Immunohistochemistry-Paraffin-NBP2-75692-img0013.jpg "Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-TXNIP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of Hela cells with TXNIP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/TXNIP-Antibody-JM60-35-Flow-Cytometry-NBP2-75692-img0001.jpg "Flow Cytometry: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of Hela cells with TXNIP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).")
![Immunocytochemistry/Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692] - Staining TXNIP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JM60-35-Immunocytochemistry-Immunofluorescence-NBP2-75692-img0002.jpg "Immunocytochemistry/Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692] - Staining TXNIP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692] - Staining TXNIP in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JM60-35-Immunocytochemistry-Immunofluorescence-NBP2-75692-img0003.jpg "Immunocytochemistry/Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692] - Staining TXNIP in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded human kidney tissue using anti-TXNIP antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JM60-35-Immunohistochemistry-Paraffin-NBP2-75692-img0005.jpg "Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded human kidney tissue using anti-TXNIP antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded human stomach cancer tissue using anti-TXNIP antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JM60-35-Immunohistochemistry-Paraffin-NBP2-75692-img0006.jpg "Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded human stomach cancer tissue using anti-TXNIP antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded mouse kidney tissue using anti-TXNIP antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JM60-35-Immunohistochemistry-Paraffin-NBP2-75692-img0007.jpg "Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded mouse kidney tissue using anti-TXNIP antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded mouse stomach tissue using anti-TXNIP antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JM60-35-Immunohistochemistry-Paraffin-NBP2-75692-img0008.jpg "Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded mouse stomach tissue using anti-TXNIP antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-TXNIP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JM60-35-Immunohistochemistry-Paraffin-NBP2-75692-img0010.jpg "Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-TXNIP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded human stomach tissue with Rabbit anti-TXNIP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JM60-35-Immunohistochemistry-Paraffin-NBP2-75692-img0011.jpg "Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded human stomach tissue with Rabbit anti-TXNIP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded mouse esophagus tissue with Rabbit anti-TXNIP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/TXNIP-Antibody-JM60-35-Immunohistochemistry-Paraffin-NBP2-75692-img0012.jpg "Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded mouse esophagus tissue with Rabbit anti-TXNIP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Western_Blot_18923.jpg)
![Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Immunocytochemistry__Immunofluorescence_18945.jpg)
![Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Simple_Western_19175.jpg)
![Western Blot MLX Antibody [Unconjugated]](https://images.novusbio.com/images2/MLX_AF4186_Western_Blot_5416.jpg)
![Immunocytochemistry MLX Antibody [Unconjugated]](https://images.novusbio.com/images2/af4186_human-mlx-affinity-purified-polyclonal-ab-immunocytochemistry-412202015556.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")