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TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free

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Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) [NBP2-00409] - Analysis using the Biotin conjugate of NBP2-00409. Staining of TWEAK R in murine 603B cell lines using anti-TWEAK R antibody. Image from verified customer review.
Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) [NBP2-00409] - Analysis using the Biotin conjugate of NBP2-00409. Staining of TWEAK R in murine 603B cell lines using anti-TWEAK R antibody. Image from verified ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC
Clone
ITEM-4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.5 mg/ml

Order Details

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View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-00409AF405
Alexa Fluor 488
NBP2-00409AF488
Alexa Fluor 532
NBP2-00409AF532
Alexa Fluor 594
NBP2-00409AF594
Alexa Fluor 647
NBP2-00409AF647
Alexa Fluor 700
NBP2-00409AF700
Alexa Fluor 750
NBP2-00409AF750
Allophycocyanin
NBP2-00409APC
Biotin
NBP1-43483-0.1mg
Biotin
NBP1-43483-0.025mg
CoraFluor 1
NBP2-00409CL1
DyLight 350
NBP2-00409UV
DyLight 488
NBP2-00409G
DyLight 550
NBP2-00409R
DyLight 594
NBP2-00409DL594
DyLight 650
NBP2-00409C
DyLight 680
NBP2-00409FR
DyLight 755
NBP2-00409IR
FITC
NBP2-00409F
HRP
NBP2-00409H
Janelia Fluor 525
NBP2-00409JF525
Janelia Fluor 549
NBP2-00409JF549
Janelia Fluor 669
NBP2-00409JF669
PE
NBP2-00409PE
PE/Atto594
NBP2-00409PEATT594
PE/Cy5.5
NBP2-00409PECY55
PE/Cy7
NBP2-00409PECY7
PerCP
NBP2-00409PCP
mFluor Violet 450 SE
NBP2-00409MFV450
mFluor Violet 500 SE
NBP2-00409MFV500
mFluor Violet 610 SE
NBP2-00409MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-00409-25ug
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Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free Summary

Immunogen
The immunogen for this antibody is TWEAKR.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF12A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
This has been tested by flow cytometric analysis on human or mouse Fn14 transfected cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Use in WB and IHC-F was reported in the scientific literature (PMID: 19473660).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for TWEAK R/TNFRSF12 Antibody (ITEM-4) - BSA Free

  • CD266 antigen
  • CD266
  • FGF-inducible 14
  • Fibroblast growth factor-inducible immediate-early response protein 14
  • Fn14
  • FN14tumor necrosis factor receptor superfamily member 12A
  • TNFRSF12
  • TNFRSF12A
  • tumor necrosis factor receptor superfamily, member 12A
  • TWEAK R
  • TWEAKR
  • tweak-receptor
  • type I transmembrane protein Fn14

Background

The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TNFRSF12A