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TWEAK R/TNFRSF12 Antibody (ITEM-4) [Biotin]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
ITEM-4
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-00409AF405
Alexa Fluor 488
NBP2-00409AF488
Alexa Fluor 532
NBP2-00409AF532
Alexa Fluor 594
NBP2-00409AF594
Alexa Fluor 647
NBP2-00409AF647
Alexa Fluor 700
NBP2-00409AF700
Alexa Fluor 750
NBP2-00409AF750
Allophycocyanin
NBP2-00409APC
Biotin
NBP1-43483-0.025mg
CoraFluor 1
NBP2-00409CL1
DyLight 350
NBP2-00409UV
DyLight 488
NBP2-00409G
DyLight 550
NBP2-00409R
DyLight 594
NBP2-00409DL594
DyLight 650
NBP2-00409C
DyLight 680
NBP2-00409FR
DyLight 755
NBP2-00409IR
FITC
NBP2-00409F
HRP
NBP2-00409H
Janelia Fluor 525
NBP2-00409JF525
Janelia Fluor 549
NBP2-00409JF549
Janelia Fluor 669
NBP2-00409JF669
PE
NBP2-00409PE
PE/Atto594
NBP2-00409PEATT594
PE/Cy5.5
NBP2-00409PECY55
PE/Cy7
NBP2-00409PECY7
PerCP
NBP2-00409PCP
Unconjugated
NBP2-00409-100ug
mFluor Violet 450 SE
NBP2-00409MFV450
mFluor Violet 500 SE
NBP2-00409MFV500
mFluor Violet 610 SE
NBP2-00409MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-00409-100ug
BSA Free
NBP2-00409-25ug
BSA Free
NBP2-00409-100ug
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TWEAK R/TNFRSF12 Antibody (ITEM-4) [Biotin] Summary

Immunogen
The immunogen for this antibody is TWEAKR.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF12A
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
This has been tested by flow cytometric analysis on human or mouse Fn14 transfected cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Readout System
Reviewed Applications
Read 1 Review rated 4
using
NBP1-43483 in the following application:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2) with 0.1% gelatin
Preservative
0.09% Sodium Azide
Purity
Protein A or G purified

Alternate Names for TWEAK R/TNFRSF12 Antibody (ITEM-4) [Biotin]

  • CD266 antigen
  • CD266
  • FGF-inducible 14
  • Fibroblast growth factor-inducible immediate-early response protein 14
  • Fn14
  • FN14tumor necrosis factor receptor superfamily member 12A
  • TNFRSF12
  • TNFRSF12A
  • tumor necrosis factor receptor superfamily, member 12A
  • TWEAK R
  • TWEAKR
  • tweak-receptor
  • type I transmembrane protein Fn14

Background

The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-43483
TWEAK R/TNFRSF12 Antibody (ITEM-4) [Biotin]
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Review for TWEAK R/TNFRSF12 Antibody (NBP1-43483) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-43483:
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Flow Cytometry TWEAK R/TNFRSF12 NBP1-43483
Enlarge 		4
reviewed by:
Guanhua Xie
 Flow Mouse 04/28/2014
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Summary

ApplicationFlow Cytometry
SpeciesMouse

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Flow Cytometry TWEAK R/TNFRSF12 NBP1-43483
Enlarge
 Guanhua Xie
04/28/2014
Application: Flow
Species: Mouse
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Bioinformatics

Gene Symbol TNFRSF12A