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Thrombospondin-4 Antibody [Unconjugated]

 (5 Publications)
 (1 Review)
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Western blot shows lysates of mouse muscle tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7860) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Thrombospondin-4 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse muscle tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7860) followed by HRP-conjugated ...read more
Thrombospondin-4 was detected in perfusion fixed frozen sections of mouse skeletal muscle using Sheep Anti-Mouse Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7860) at 5 µg/mL overnight at ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier Free
AF7860-SP
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Thrombospondin-4 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Thrombospondin-4
Gln27-Asn963 (predicted)
Accession # Q9Z1T2
Specificity
Detects mouse Thrombospondin-4 in direct ELISAs and Western blots. In direct ELISAs, aproximately 10% cross-reactivity with recombinant human Thrombospondin-4 is observed, and less than 1% cross-reactivity with recombinant mouse Thrombospondin-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
THBS4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF7860 in the following applications:
Publications
Read Publications using
AF7860 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thrombospondin-4 Antibody [Unconjugated]

  • THBS4
  • thrombospondin 4
  • Thrombospondin4
  • Thrombospondin-4
  • TSP4
  • TSP-4

Background

Mouse Thrombospondin-4 (TSP-4) is a 120-130 kDa adhesive glycoprotein that belongs to the thrombospondin family of proteins.  It is 963 amino acids (aa) in length and highly modular in structure, containing one Lamnin G-like domain, four EGF-like domains and eight type 3 TSP domains.  It circulates as a disulfide-linked homopentamer, and is up-regulated in the heart in response to ischemic injury and pathology. Mature mouse TSP-4 is 93% aa identical to human TSP4.     

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Thrombospondin-4 Antibody (AF7860)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, Immunohistochemistry, Western Blot.


Filter By Application
IHC
(1)
Immunohistochemistry
(1)
Western Blot
(1)
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Filter By Species
Human
(1)
Mouse
(3)
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Showing Publications 1 - 5 of 5.
Publications using AF7860 Applications Species
Zarén, P;Gawlik, KI; Thrombospondin-4 deletion does not exacerbate muscular dystrophy in ?-sarcoglycan-deficient and laminin ?2 chain-deficient mice Scientific reports 2024-06-26 [PMID: 38926599] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Sachin S. Deshpande, Subash C. Malik, Pasquale Conforti, Jia-di Lin, Yu-Hsuan Chu, Suvra Nath et al. P75 neurotrophin receptor controls subventricular zone neural stem cell migration after stroke Cell and Tissue Research 3/1/2022 [PMID: 34698916]
LingNan He, WeiJun Wang, HuiYing Shi, Chen Jiang, HaiLing Yao, YuRui Zhang et al. THBS4/integrin alpha 2 axis mediates BM-MSCs to promote angiogenesis in gastric cancer associated with chronic Helicobacter pylori infection Aging (Albany NY) 8/15/2021 [PMID: 34390328]
L Pous, SS Deshpande, S Nath, S Mezey, SC Malik, S Schildge, C Bohrer, K Topp, D Pfeifer, F Fernández-, S Doostkam, DK Galanakis, V Taylor, K Akassoglou, C Schachtrup Fibrinogen induces neural stem cell differentiation into astrocytes in the subventricular zone via BMP signaling Nat Commun, 2020-01-31;11(1):630. 2020-01-31 [PMID: 32005867] (IHC, Human, Mouse) IHC Human, Mouse
AB Hanker, MV Estrada, G Bianchini, PD Moore, J Zhao, F Cheng, L Gianni, JP Koch, DR Tyson, V Sanchez, BN Rexer, M Sanders, Z Zhao, T Stricker, CL Arteaga Extracellular matrix/integrin signaling promotes resistance to combined inhibition of HER2 and PI3K in HER2+ breast cancer Cancer Res., 2017-04-10;0(0):. 2017-04-10 [PMID: 28396358] (Western Blot, Mouse) Western Blot Mouse

Review for Thrombospondin-4 Antibody (AF7860) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using AF7860:
Filter by Applications
IHC
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
marjorie maillet
 IHC Rat 03/31/2017
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Summary

ApplicationImmunohistochemistry
Sample TestedMEFs whole cell lysate
SpeciesRat

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Recent Reviews

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 marjorie maillet
03/31/2017
Application: IHC
Species: Rat
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Bioinformatics

Gene Symbol THBS4
Uniprot