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Versican Antibody [Unconjugated] - Isoform V0

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Versican was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human Versican Isoform V0 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3054) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Versican was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human Versican Isoform V0 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3054) for 3 ...read more
Versican was detected in immersion fixed U‑251 MG human glioblastoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Goat Anti-Human Versican Isoform V0 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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BAF3054
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AF3054-SP
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Versican Antibody [Unconjugated] - Isoform V0 Summary

Immunogen
E. coli-derived recombinant human Versican isoform V0
Gly1344-Asp1554
Accession # P13611
Specificity
Detects human Versican isoform V0 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VCAN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF3054 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Versican Antibody [Unconjugated] - Isoform V0

  • Chondroitin sulfate proteoglycan 2WGN
  • Chondroitin sulfate proteoglycan core protein 2
  • CSPG2
  • CSPG2DKFZp686K06110
  • ERVR
  • GHAP
  • Glial hyaluronate-binding protein
  • Large fibroblast proteoglycan
  • PG-M
  • PG-MWGN1
  • VCAN
  • versican core protein
  • versican proteoglycan
  • Versican

Background

Versican (versatile proteoglycan; also known as PG-M) is a 500-600 kDa, secreted member of the Aggrecan/Versican proteoglycan family. It is a variably glycanated, hyaluronan-binding, chondroitin sulfate-containing molecule that likely serves an anti-adhesion function and regulates the hydration properties of pericellular matrices. Human Versican is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region (G1; aa 21-346), two large, alternately spliced GAG attachment domains (GAG alpha ; aa 348-1335; GAG beta aa 1336-3088) and a C-terminal selectin-like region (G3; aa 3089-3396). Over aa 1344-1554, human Versican shares 80% and 60% aa sequence identity with canine and mouse Versican, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Versican Antibody (AF3054)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC, IHC, Western Blot.


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ICC
(1)
IHC
(2)
Western Blot
(2)
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(5)
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Showing Publications 1 - 8 of 8.
Publications using AF3054 Applications Species
Riabinin, A;Kalabusheva, E;Khrustaleva, A;Akulinin, M;Tyakht, A;Osidak, E;Chermnykh, E;Vasiliev, A;Vorotelyak, E; Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics Scientific reports 2023-08-30 [PMID: 37648686] (ICC, Human) ICC Human
Alena Kashirina, Alena Gavrina, Artem Mozherov, Dmitriy Kozlov, Daria Kuznetsova, Ekaterina Vorotelyak et al. FLIM for Evaluation of Difference in Metabolic Status between Native and Differentiated from iPSCs Dermal Papilla Cells Cells 2022-09-01 [PMID: 36078136]
B Sitaš, M Bobi?-Raso, G Mrak, S Trnski, M Krbot Skor, D Oreškovi?, V Knezovi?, Ž Petelin Ga, Z Petanjek, G Šimi?, D Kolenc, N Jovanov Mi Reorganization of the Brain Extracellular Matrix in Hippocampal Sclerosis International Journal of Molecular Sciences, 2022-07-25;23(15):. 2022-07-25 [PMID: 35897768] (IHC, Human) IHC Human
Kazadi N. Mutoji, Mingxia Sun, Garrett Elliott, Isabel Y. Moreno, Clare Hughes, Tarsis F. Gesteira et al. Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice International Journal of Molecular Sciences 2021-05-27 [PMID: 34071909]
S Taylor, M Whitfield, J Barratt, A Didangelos The Metalloproteinase ADAMTS5 Is Expressed by Interstitial Inflammatory Cells in IgA Nephropathy and Is Proteolytically Active on the Kidney Matrix J. Immunol., 2020-09-11;0(0):. 2020-09-11 [PMID: 32917786] (IHC, Human) IHC Human
Yumiko Miyazaki, Akihito Horie, Hirohiko Tani, Masashi Ueda, Asuka Okunomiya, Koh Suginami et al. Versican V1 in human endometrial epithelial cells promotes BeWo spheroid adhesion in vitro Reproduction 2019-01-01 [PMID: 30394708]
RD Kenagy, S Kikuchi, SP Evanko, MS Ruiter, M Piola, A Longchamp, M Pesce, M Soncini, S Deglise, GB Fiore, JA Haefliger, TA Schmidt, MW Majesky, M Sobel, TN Wight Versican is differentially regulated in the adventitial and medial layers of human vein grafts PLoS ONE, 2018-09-28;13(9):e0204045. 2018-09-28 [PMID: 30265729] (Western Blot, Human) Western Blot Human
Kischel P, Waltregny D, Dumont B, Turtoi A, Greffe Y, Kirsch S, De Pauw E, Castronovo V Versican overexpression in human breast cancer lesions: known and new isoforms for stromal tumor targeting. Int. J. Cancer, 2010-02-01;126(3):640-50. 2010-02-01 [PMID: 19662655] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol VCAN
Uniprot