I have synovial fluid sample and I want to work on Western bloting can you support me ,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?

Name: Human COMP Quantikine ELISA Kit
Brand: Novus Biologicals
SKU: DCMP0
Price: 635 USD

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Accepted Answer

We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	ELISA
Conjugate	HRP

Background	The Quantikine Human COMP immunoassay is a 4.5 hour solid phase ELISA designed to measure human COMP in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human COMP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human COMP showed linear curves that were parallel to the standard curves obtained u...sing the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring COMP. Show More
Specificity	Natural and recombinant human COMP
Source	N/A
Assay Type	Solid Phase Sandwich ELISA
Inter-Assay	See PDF Datasheet for details
Intra-Assay	See PDF Datasheet for details
Spike Recovery	See PDF Datasheet for details
Sample Volume	See PDF Datasheet for details
Gene	COMP

Publications using DCMP0	Applications	Species
Nishida, Y;Hashimoto, Y;Orita, K;Nishino, K;Kinoshita, T;Iida, K;Nakamura, H; Longitudinal measurement of serum cartilage oligomeric matrix protein can detect the progression of cartilage degeneration in anterior cruciate ligament reconstruction patients Asia-Pacific journal of sports medicine, arthroscopy, rehabilitation and technology 2024-07-01 [PMID: 39091893] (Human)		Human
HJ Kim, HC Lee The Effect of 13 Weeks Long-Distance Bicycle Riding on Inflammatory Response Indicators Related to Joint Cartilage and Muscle Damage International Journal of Environmental Research and Public Health, 2022-12-06;19(23):. 2022-12-06 [PMID: 36498391] (Human)		Human
C Papaneophy, A Alabajos-C, E Viosca-Her, C Calvis, M Costa, AE Christodou, A Kroushovsk, A Lapithis, VM Lapithi, I Papayianni, A Christou, R Messeguer, C Giannaki, K Felekkis Associations between serum biomarkers of cartilage metabolism and serum hyaluronic acid, with risk factors, pain categories, and disease severity in knee osteoarthritis: a pilot study Bmc Musculoskeletal Disorders, 2022-03-02;23(1):195. 2022-03-02 [PMID: 35236298] (Human)		Human
SW Jhan, CJ Wang, KT Wu, KK Siu, JY Ko, WC Huang, WY Chou, JH Cheng Comparison of Extracorporeal Shockwave Therapy with Non-Steroid Anti-Inflammatory Drugs and Intra-Articular Hyaluronic Acid Injection for Early Osteoarthritis of the Knees Biomedicines, 2022-01-18;10(2):. 2022-01-18 [PMID: 35203417] (Human)		Human
Y Fu, Y Huang, Z Yang, Y Chen, J Zheng, C Mao, Z Li, Z Liu, B Yu, T Li, M Wang, C Xu, Y Zhou, G Zhao, Y Jia, W Guo, X Jia, T Zhang, L Li, Z Liu, S Guo, M Ma, H Zhang, B Liu, J Du, W Wang, C Tang, P Gao, Q Xu, X Wang, J Liu, J Sun, W Kong Cartilage oligomeric matrix protein is an endogenous &beta-arrestin-2-selective allosteric modulator of AT1 receptor counteracting vascular injury Cell Research, 2021-01-28;0(0):. 2021-01-28 [PMID: 33510386] (Human)		Human
K Winsz-Szcz, K Ku?nik-Tro, A Gruenpeter, M Wojdas, K D?bkowska, K Olczyk Association of Circulating COMP and YKL-40 as Markers of Metabolic Changes of Cartilage with Adipocytokines in Juvenile Idiopathic Arthritis Metabolites, 2020-02-10;10(2):. 2020-02-10 [PMID: 32050571] (Human)		Human
L Sun, Y Wang, L Wang, B Yao, T Chen, Q Li, Z Liu, R Liu, Y Niu, T Song, Q Liu, K Tu Resolvin D1 prevents epithelial-mesenchymal transition and reduces the stemness features of hepatocellular carcinoma by inhibiting paracrine of cancer-associated fibroblast-derived COMP J. Exp. Clin. Cancer Res., 2019-04-18;38(1):170. 2019-04-18 [PMID: 30999932] (Human)		Human
VN Nfonsam, H Jecius, D Chen, PN Omesiete, AN Ewongwo, E Elquza, AJ Scott, J Jandova Increasing Incidence of Colon Cancer in the Young: Assessing the Tumor Biology J. Am. Coll. Surg., 2019-04-14;0(0):. 2019-04-14 [PMID: 30995524] (Human)		Human
S Pérez-Garc, M Carrión, R Villanueva, T Hermida-Gó, M Fernández-, M Mellado, FJ Blanco, Y Juarranz, RP Gomariz Wnt and RUNX2 mediate cartilage breakdown by osteoarthritis synovial fibroblast-derived ADAMTS-7 and -12 J. Cell. Mol. Med., 2019-03-22;0(0):. 2019-03-22 [PMID: 30903650] (Human)		Human
M Azukizawa, H Ito, Y Hamamoto, T Fujii, Y Morita, A Okahata, T Tomizawa, M Furu, K Nishitani, S Kuriyama, S Nakamura, H Yoshitomi, T Nakatani, T Tsuboyama, M Hamaguchi, S Matsuda, T Yasuda The Effects of Well-Rounded Exercise Program on Systemic Biomarkers Related to Cartilage Metabolism Cartilage, 2018-04-12;0(0):1947603518767. 2018-04-12 [PMID: 29644876] (Human)		Human
HL Lopez, SM Habowski, JE Sandrock, B Raub, A Kedia, EJ Bruno, TN Ziegenfuss Effects of dietary supplementation with a standardized aqueous extract of Terminalia chebula fruit (AyuFlex(ï¿½)) on joint mobility, comfort, and functional capacity in healthy overweight subjects: a randomized placebo-controlled clinical trial BMC Complement Altern Med, 2017-10-02;17(1):475. 2017-10-02 [PMID: 28969626] (Human)		Human
E Das Gupta, WR Ng, SF Wong, AK Bhurhanude, SS Yeap Correlation of serum cartilage oligometric matrix protein (COMP) and interleukin-16 (IL-16) levels with disease severity in primary knee osteoarthritis: A pilot study in a Malaysian population PLoS ONE, 2017-09-14;12(9):e0184802. 2017-09-14 [PMID: 28910372] (Human)		Human
Show All 12 Publications. Collapse Publications.

Human COMP Quantikine ELISA Kit

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