![Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Representative photomicrographs showing Thrombospondin-1 (A) immunostaining in duodenal sections of 1 non-CD controls (NC) and 1 active celiac disease (ACD) patient. Isotype control staining is also shown. Right panels: positive cells were counted in at least 5 fields/section of duodenal samples of 5 NC and 5 ACD patients and expressed as mean +/- SD of all experiments. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0100980), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-NB100-2059-img0015.jpg "Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Representative photomicrographs showing Thrombospondin-1 (A) immunostaining in duodenal sections of 1 non-CD controls (NC) and 1 active celiac disease (ACD) patient. Isotype control staining is also shown. Right panels: positive cells were counted in at least 5 fields/section of duodenal samples of 5 NC and 5 ACD patients and expressed as mean +/- SD of all experiments. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0100980), licensed under a CC-BY license.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, B/N |
| Clone | A6.1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. |
| Localization | Secretory granules, Golgi complex, endoplasmic reticulum |
| Specificity | This is specific to 450 kD protein, a non-reduced form, and 170 to 180 kD protein, a reduced form, of Thrombospondin. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | THBS1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT. Use in Blocking/Neutralizing reported in scientific literature (PMID: 19509293). Use in Western blot reported in reported in scientific literature (PMID: 27447109). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24971453). |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4), 0.2% BSA, Tween-20 |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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