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TGF-beta 2 Antibody (8607) [Unconjugated]

 (10 Publications)
 (1 Review)
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TGF-beta 2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-TGF-beta 2 Monoclonal Antibody (Catalog # MAB612) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
TGF-beta 2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-TGF-beta 2 Monoclonal Antibody (Catalog # MAB612) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, IHC, ELISA(Cap)
Clone
8607
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB612-SP
Carrier Free
MAB612-500
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TGF-beta 2 Antibody (8607) [Unconjugated] Summary

Immunogen
Porcine TGF-beta 1.2
Specificity
Detects TGF-beta 2 from multiple species in ELISAs and Western blots. In ELISAs, no cross-reactivity or interfereence with recombinant human (rh) TGF-alpha , recombinant porcineTGF-beta 1, rhTGF-beta 1, recombinant canine TGF-beta 3, recombinant amphibian TGF-beta 5, rhTGF-beta RII, or rhTGF-beta RIII is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TGFB2
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: TGF-beta 2 Biotinylated Antibody (Catalog number BAF302)
Standard: Recombinant Human TGF-beta 2 (Catalog number 302-B2)
Reviewed Applications
Read 1 Review rated 5
using
MAB612 in the following applications:
Publications
Read Publications using
MAB612 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta 2 Antibody (8607) [Unconjugated]

  • BSC-1 cell growth inhibitor
  • cetermin
  • Glioblastoma-derived T-cell suppressor factor
  • G-TSF
  • MGC116892
  • polyergin
  • TGFB2
  • TGFbeta 2
  • TGF-beta 2
  • TGF-beta2
  • TGF-beta-2
  • transforming growth factor beta-2
  • transforming growth factor, beta 2

Background

The active form of TGF-beta 2 is a disulfide-linked homodimer which is generated by activation of the latent TGF-beta 2 complex. TGF-beta s are multifunctional cytokines that activate or repress multiple genes in a wide variety of cell types. Depending on the cell context, TGF-beta can control many cellular processes including growth, morphogenesis, and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TGF-beta 2 Antibody (8607) [Unconjugated]
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Publications for TGF-beta 2 Antibody (MAB612)(10)

We have publications tested in 2 confirmed species: Human, Xenograft.

We have publications tested in 3 applications: ELISA Development, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
Neutralization
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(4)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using MAB612 Applications Species
Y Sui, W Hu, W Zhang, D Li, H Zhu, Q You, R Zhu, Q Yi, T Tang, L Gao, S Zhu, T Yang Insights into homeobox B9: a propeller for metastasis in dormant prostate cancer progenitor cells British Journal of Cancer, 2021-07-10;0(0):. 2021-07-10 [PMID: 34247196] (Western Blot, Xenograft) Western Blot Xenograft
Carlos de Oliveira R, Tye G, Sampaio L et al. TGF beta 1 and TGF beta 2 proteins in corneas with and without stromal fibrosis: Delayed regeneration of apical epithelial growth factor barrier and the epithelial basement membrane in corneas with stromal fibrosis Experimental Eye Research 10/1/2020 [PMID: 33263285]
N Kanaji, M Yokohira, N Watanabe, N Kadowaki, S Bandoh Transforming Growth Factor-Beta Produced by Non-small Cell Lung Cancer Cells Contributes to Lung Fibroblast Contractile Phenotype Anticancer Res., 2018-04-01;38(4):2007-2014. 4/1/2018 [PMID: 29599317]
Yin S, Fan Y, Zhang H, Zhao Z, Hao Y, Li J, Sun C, Yang J, Yang Z, Yang X, Lu J, Xi J Differential TGFbeta pathway targeting by miR-122 in humans and mice affects liver cancer metastasis. Nat Commun, 2016-03-18;7(0):11012. 2016-03-18 [PMID: 26987776] (Western Blot, Human) Western Blot Human
Markus J. Riemenschneider, Maria Hirblinger, Arabel Vollmann-Zwerenz, Peter Hau, Martin A. Proescholdt, Frank Jaschinski et al. TGF-beta isoforms in cancer: Immunohistochemical expression and Smad-pathway-activity-analysis in thirteen major tumor types with a critical appraisal of antibody specificity and immunohistochemistry assay validity Oncotarget 9/29/2015 [PMID: 26450853]
Kameswara Rao Badri, Ming Yue, Oscar A. Carretero, Sree Latha Aramgam, Jun Cao, Stephen Sharkady et al. Blood pressure homeostasis is maintained by a P311–TGF-beta axis Journal of Clinical Investigation 10/1/2013 [PMID: 24091331]
Oana Tudoran, Olga Soritau, Ovidiu Balacescu, Loredana Balacescu, Cornelia Braicu, Meda Rus et al. Early transcriptional pattern of angiogenesis induced by EGCG treatment in cervical tumour cells Journal of Cellular and Molecular Medicine 3/1/2012 [PMID: 21609393]
Kawahara S, Hata Y, Kita T, Arita R, Miura M, Nakao S, Mochizuki Y, Enaida H, Kagimoto T, Goto Y, Hafezi-Moghadam A, Ishibashi T Potent inhibition of cicatricial contraction in proliferative vitreoretinal diseases by statins. Diabetes, 2008-07-03;57(10):2784-93. 2008-07-03 [PMID: 18599521] (Neutralization, Human) Neutralization Human
Wen FQ, Liu X, Kobayashi T, Abe S, Fang Q, Kohyama T, Ertl R, Terasaki Y, Manouilova L, Rennard SI Interferon-gamma inhibits transforming growth factor-beta production in human airway epithelial cells by targeting Smads. Am. J. Respir. Cell Mol. Biol., 2004-01-12;30(6):816-22. 2004-01-12 [PMID: 14722222] (ELISA Development, Human) ELISA Development Human
Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells. Am. J. Physiol. Lung Cell Mol. Physiol., 2002-05-01;282(5):L1049-56. 2002-05-01 [PMID: 11943670] (Human) Human

Review for TGF-beta 2 Antibody (MAB612) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB612:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot TGF-beta 2 MAB612
Enlarge 		5
reviewed by:
Verified Customer
 WB Human 04/25/2017
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Summary

ApplicationWestern Blot
Sample TestedMDA-MB-231 Cell Lysate
SpeciesHuman
LotHL0415101

Comments

CommentsMDA-MB-231 cell extract probed with Mouse anti-Human TGF-beta 2 antibody (#MAB612) at 1 ug/mL, followed by Goat anti-Mouse secondary antibody at 1:5,000 dilution. PVDF membrane blocked with 5% BSA-PBS.

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FAQs for TGF-beta 2 Antibody (MAB612) (0)

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Secondary Antibodies

 

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Western Blot TGF-beta 2 MAB612
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 Verified Customer
04/25/2017
Application: WB
Species: Human
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Bioinformatics

Gene Symbol TGFB2