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TBC1D4 Antibody (OTI5E6) - Azide and BSA Free

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Western Blot: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TBC1D4.
Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-TBC1D4 ...read more
Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - Analysis of Hela cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - Analysis of Jurkat cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, CyTOF-ready
Clone
OTI5E6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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TBC1D4 Antibody (OTI5E6) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human TBC1D4 (NP_0055647) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TBC1D4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
Theoretical MW
146.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TBC1D4 Antibody (OTI5E6) - Azide and BSA Free

  • Akt substrate of 160 kDa
  • AS160Acrg embryonic lethality minimal region ortholog
  • DKFZp779C0666
  • KIAA0603TBC (Tre-2, BUB2, CDC16) domain-containing protein
  • TBC1 domain family member 4
  • TBC1 domain family, member 4

Background

AS160, also known as TBC1D4, is a 160 kDa Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4. AS-160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking. Insulin-stimulated phosphorylation of AS160 on threonine 642 is critical for mediating GLUT4 translocation in fat and muscle cells, via inactivation of Rab GAP function. Phosphorylation of Thr642 is also important in mediating TBC1D4 redistribution from low density microsomes to the cytosol in adipocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TBC1D4 Antibody (NBP2-74462) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TBC1D4