![Western Blot: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of SOX9 on NIH/3T3 cell lysates with Rabbit anti-SOX9 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 56 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Western-Blot-NBP2-67690-img0010.jpg "Western Blot: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of SOX9 on NIH/3T3 cell lysates with Rabbit anti-SOX9 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 56 kDa Observed band size: 70 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ChIP, Flow, ICC/IF, IHC, IP |
| Clone | SN74-09 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant protein within human SOX9 aa 140-340. (SwissProt: P48436 Human; SwissProt: Q04887 Mouse; SwissProt: F1LYL9 Rat) |
| Localization | Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SOX9 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SOX9 Antibody (NBP2-67690)Find related products by research area.
|
|
Role of GFAP in astrocytes: Lessons from induced pluripotent stem cells in Alexander disease patients By Michalina Hanzel, PhDAlexander disease is a progressive and fatal neurological disease with phenotypes ranging from myelination abnormalities, gait ataxia and megalencephaly to predisposition to seizures. It is an ... Read full blog post. |
|
Deriving neural precursor cells from human induced pluripotent stem cells By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions... Read full blog post. |
|
The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura. It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol... Read full blog post. |
|
SOX9 - Be careful, I can reverse your gender! SOX9 is a member of the SOX family of HMG DNA-binding domain transcription factors. The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. cAMP and protein kinase A (PKA) st... Read full blog post. |
|
Using the Hif-1 Alpha Antibody in Prostate Cancer Research The Hypoxia-inducible Factor 1 (HIF1) protein is a heterodimeric transcription factor which plays an important role in mammalian oxygen homeostasis in conditions of hypoxia, or low oxygen concentration. HIF-1 alpha antibody reagents are widely used in... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SOX9 |
![Immunocytochemistry/Immunofluorescence: SOX9 Antibody (SN74-09) [NBP2-67690] - Staining SOX9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunocytochemistry-Immunofluorescence-NBP2-67690-img0007.jpg "Immunocytochemistry/Immunofluorescence: SOX9 Antibody (SN74-09) [NBP2-67690] - Staining SOX9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0017.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of Hela cells with SOX9 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Flow-Cytometry-NBP2-67690-img0001.jpg "Flow Cytometry: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of Hela cells with SOX9 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Western Blot: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of SOX9 on SW480 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Western-Blot-NBP2-67690-img0008.jpg "Western Blot: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of SOX9 on SW480 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Western Blot: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of SOX9 on different lysates with Rabbit anti-SOX9 antibody at 1/500 dilution.Lane 1: SW480 cell lysateLane 2: A431 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 56 kDaObserved band size: 70 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Western-Blot-NBP2-67690-img0009.jpg "Western Blot: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of SOX9 on different lysates with Rabbit anti-SOX9 antibody at 1/500 dilution.Lane 1: SW480 cell lysateLane 2: A431 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 56 kDaObserved band size: 70 kDaExposure time: 2 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: SOX9 Antibody (SN74-09) [NBP2-67690] - Staining SOX9 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunocytochemistry-Immunofluorescence-NBP2-67690-img0002.jpg "Immunocytochemistry/Immunofluorescence: SOX9 Antibody (SN74-09) [NBP2-67690] - Staining SOX9 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-SOX9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0003.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-SOX9 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human tonsil tissue using anti-SOX9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0004.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human tonsil tissue using anti-SOX9 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded mouse colon tissue using anti-SOX9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0005.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded mouse colon tissue using anti-SOX9 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded mouse spleen tissue using anti-SOX9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0006.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded mouse spleen tissue using anti-SOX9 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0011.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human thyroid tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0012.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human thyroid tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human breast tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0013.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human breast tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human small intestine tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0014.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human small intestine tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0015.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/SOX9-Antibody-SN74-09-Immunohistochemistry-Paraffin-NBP2-67690-img0016.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (SN74-09) [NBP2-67690] - Analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-SOX9 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry RUNX2/CBFA1 Antibody (232902) [Unconjugated]](https://images.novusbio.com/images2/RUNX2_MAB2006_Immunocytochemistry_13309.jpg)
![Bioactivity BMP-2 [Unconjugated]](https://images.novusbio.com/images/protein/355-bm_recombinant-human-mouse-rat-bmp-2-protein-bioactivity-181120209742.jpg)
![Immunocytochemistry SOX10 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX10_AF2864_Immunocytochemistry__Immunofluorescence_17799.jpg)
![Immunocytochemistry SOX10 Antibody [Unconjugated]](https://images.novusbio.com/images2/af2864_human-sox10-affinity-purified-polyclonal-ab-immunocytochemistry-3032021155556.jpg)
![Immunohistochemistry SOX10 Antibody [Unconjugated]](https://images.novusbio.com/images2/af2864_human-sox10-affinity-purified-polyclonal-ab-immunohistochemistry-303202115544.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![SDS-PAGE IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_40.jpg)
![Bioactivity IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_41.jpg)
![Mass Spectrometry IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_42.jpg)
![SDS-PAGE BMP-4 [Unconjugated]](https://images.novusbio.com/images2/BMP-4_314-BP_100.jpg)
![Bioactivity BMP-4 [Unconjugated]](https://images.novusbio.com/images2/314-bp_recombinant-human-bmp-4-protein-1882021123744.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")