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SOX10 Antibody [Unconjugated]

Images

 
SOX10 was detected in immersion fixed SK‑Mel‑28 human malignant melanoma cell line using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 15 µg/mL for 3 ...read more
SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 10 µg/mL ...read more
SOX10 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 15 µg/mL for 1 hour at ...read more
PDGFR alpha driven brain tumors display features of high grade glioma.(a–g) Histopathological analysis of tumor areas by H&E staining shows a high concentration of mitotic figures (a, arrows), high cellularity and ...read more
Adult dynamin 2-depleted Schwann cells are replaced by dynamin 2-positive Schwann cells in remyelinated nerves.(A) Immunostainings for DNM2, SOX10 (SCs) and YFP (reporter-recombined SCs) on SN cross-sections of control* ...read more
Schwann cells lacking dynamin 2 show impaired cell cycle progression, reduced mitosis rate, and cytokinesis defects.(A) Schematic representation of the cell cycle phases marked by Ki-67 and EdU. (B) EdU-labeling, ...read more
PDGFR alpha driven brain tumors display features of high grade glioma.(a–g) Histopathological analysis of tumor areas by H&E staining shows a high concentration of mitotic figures (a, arrows), high cellularity and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price
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SOX10 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX10
Met1-Ala118
Accession # P56693
Specificity
Detects human SOX10 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX10
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 5
using
AF2864 in the following applications:

Publications
Read Publications using
AF2864 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX10 Antibody [Unconjugated]

  • DOM
  • MGC15649
  • PCWH
  • SOX10
  • SRY (sex determining region Y)-box 10
  • SRY-related HMG-box gene 10
  • transcription factor SOX-10
  • WS4
  • WS4C
  • WS4mouse, human homolog of

Background

SOX10 belongs to the SOX family of transcription factors with diverse roles during development. In the central nervous system (CNS), SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX10 Antibody (AF2864)(117)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Transgenic Mouse, Xenograft.

We have publications tested in 14 applications: FACS, Flow Cytometry, ICC, ICC/IF, IHC, IHC-F, IHC-Fr, IHC-P, IHC/IF, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Proximity Ligation Assay (PLA), Western Blot.


Filter By Application
FACS
(1)
Flow Cytometry
(1)
ICC
(17)
ICC/IF
(1)
IHC
(23)
IHC-F
(1)
IHC-Fr
(4)
IHC-P
(2)
IHC/IF
(1)
Immunocytochemistry
(1)
Immunohistochemistry
(3)
Immunoprecipitation
(1)
Proximity Ligation Assay (PLA)
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(24)
Mouse
(29)
Rat
(4)
Chicken
(1)
Transgenic Mouse
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 117. Show All 117 Publications.
Publications using AF2864 Applications Species
Wang, L;Wang, C;Moriano, JA;Chen, S;Zuo, G;Cebrián-Silla, A;Zhang, S;Mukhtar, T;Wang, S;Song, M;de Oliveira, LG;Bi, Q;Augustin, JJ;Ge, X;Paredes, MF;Huang, EJ;Alvarez-Buylla, A;Duan, X;Li, J;Kriegstein, AR; Molecular and cellular dynamics of the developing human neocortex at single-cell resolution bioRxiv : the preprint server for biology 2024-08-04 [PMID: 39131371] (Immunohistochemistry, Xenograft) Immunohistochemistry Xenograft
van Baarle, L;De Simone, V;Schneider, L;Santhosh, S;Abdurahiman, S;Biscu, F;Schneider, R;Zanoletti, L;Siqueira de Mello, R;Verbandt, S;Hu, Z;Stakenborg, M;Ke, BJ;Stakenborg, N;Salvador Laureano, R;García-Reyes, B;Henn, J;Toma, M;Vanmechelen, M;Boeckxstaens, G;De Smet, F;Garg, AD;Ibiza, S;Tejpar, S;Wehner, S;Matteoli, G; IL-1R signaling drives enteric glia-macrophage interactions in colorectal cancer Nature communications 2024-07-19 [PMID: 39030280] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Verstraelen, P;Van Remoortel, S;De Loose, N;Verboven, R;Garcia-Diaz Barriga, G;Christmann, A;Gries, M;Bessho, S;Li, J;Guerra, C;Tükel, Ç;Ibiza Martinez, S;Schäfer, KH;Timmermans, JP;De Vos, WH; Serum amyloid A3 fuels a feed-forward inflammatory response to the bacterial amyloid curli in the enteric nervous system Cellular and molecular gastroenterology and hepatology 2024-03-29 [PMID: 38556049] (Immunocytochemistry, Human) Immunocytochemistry Human
Tsartsalis, S;Sleven, H;Fancy, N;Wessely, F;Smith, AM;Willumsen, N;Cheung, TKD;Rokicki, MJ;Chau, V;Ifie, E;Khozoie, C;Ansorge, O;Yang, X;Jenkyns, MH;Davey, K;McGarry, A;Muirhead, RCJ;Debette, S;Jackson, JS;Montagne, A;Owen, DR;Miners, JS;Love, S;Webber, C;Cader, MZ;Matthews, PM; A single nuclear transcriptomic characterisation of mechanisms responsible for impaired angiogenesis and blood-brain barrier function in Alzheimer's disease Nature communications 2024-03-12 [PMID: 38472200] (Flow Cytometry, Human) Flow Cytometry Human
Robinson, BG;Oster, BA;Robertson, K;Kaltschmidt, JA; Loss of ASD-related molecule Cntnap2 affects colonic motility in mice Frontiers in neuroscience 2023-11-09 [PMID: 38027494] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Guo, R;Han, D;Song, X;Gao, Y;Li, Z;Li, X;Yang, Z;Xu, Z; Context-dependent regulation of Notch signaling in glial development and tumorigenesis Science advances 2023-11-10 [PMID: 37948517] (IHC, Mouse) IHC Mouse
Li J, Jaiswal MK, Chien JF et al. Divergent single cell transcriptome and epigenome alterations in ALS and FTD patients with C9orf72 mutation Nat Commun 2023-09-15 [PMID: 37714849]
Frazel, PW;Labib, D;Fisher, T;Brosh, R;Pirianian, N;Marchildon, A;Boeke, JD;Fossati, V;Liddelow, SA; Longitudinal scRNA-seq analysis in mouse and human informs optimization of rapid mouse astrocyte differentiation protocols Nature neuroscience 2023-09-11 [PMID: 37697111] (ICC, Human) ICC Human
Clayton, BLL;Barbar, L;Sapar, M;Rusielewicz, T;Kalpana, K;Migliori, B;NYSCF Global Stem Cell Array® Team, ;Paull, D;Brenner, K;Moroziewicz, D;Sand, IK;Casaccia, P;Tesar, PJ;Fossati, V; Patient iPSC models reveal glia-intrinsic phenotypes in multiple sclerosis bioRxiv : the preprint server for biology 2023-08-02 [PMID: 37577713] (ICC, Human) ICC Human
Zhenqing Liu, Jianfei Chao, Cheng Wang, Guihua Sun, Daniel Roeth, Wei Liu et al. Astrocytic response mediated by the CLU risk allele inhibits OPC proliferation and myelination in a human iPSC model Cell Reports 2023-08-29 [PMID: 37494190]
Show All 117 Publications.

Reviews for SOX10 Antibody (AF2864) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF2864:
Filter by Applications
IHC-Fr
(1)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen SOX10 AF2864
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 05/09/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedEmbryonic spinal cord
SpeciesMouse
LotVRY0516091
Immunohistochemistry-Paraffin SOX10 AF2864
Enlarge
5
reviewed by:
Kelli Jordan
IHC-P Mouse 02/26/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Pancreas
SpeciesMouse
LotVRY081810C

Comments

CommentsAntigen retrieval: HIER, pH 6
Block: 1 hour in 2% normal donkey serum, 2 mg/ml BSA; 0.2% triton-x
Primary: 10 ug/ml in blocking buffer, overnight at 4C
Secondary: Donkey anti-goat DyLight 650, 1:300 for 1 hour

Product General Protocols

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WB Video Protocol

FAQs for SOX10 Antibody (AF2864) (0)

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
05/09/2019
Application: IHC-Fr
Species: Mouse

Kelli Jordan
02/26/2019
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol SOX10
Uniprot