Name: Collagen II Antibody (5B2.5) - BSA Free
Brand: Novus Biologicals
SKU: NB600-844
Price: 449 USD

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, Ch, RbSpecies Glossary
Applications	WB, ICC/IF, IHC
Clone	5B2.5
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Format	BSA Free
Concentration	1.0 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB600-844AF350

Alexa Fluor 405 NB600-844AF405

Alexa Fluor 488 NB600-844AF488

Alexa Fluor 532 NB600-844AF532

Alexa Fluor 594 NB600-844AF594

Alexa Fluor 647 NB600-844AF647

Alexa Fluor 700 NB600-844AF700

Alexa Fluor 750 NB600-844AF750

Allophycocyanin NB600-844APC

Biotin NB600-844B

CoraFluor 1 NB600-844CL1

DyLight 350 NB600-844UV

DyLight 405 NB600-844V

DyLight 488 NB600-844G

DyLight 550 NB600-844R

DyLight 594 NB600-844DL594

DyLight 650 NB600-844C

DyLight 680 NB600-844FR

DyLight 755 NB600-844IR

FITC NB600-844F

HRP NB600-844H

Janelia Fluor 525 NB600-844JF525

Janelia Fluor 549 NB600-844JF549

Janelia Fluor 585 NB600-844JF585

Janelia Fluor 635 NB600-844JF635

Janelia Fluor 646 NB600-844JF646

Janelia Fluor 669 NB600-844JF669

PE NB600-844PE

PerCP NB600-844PCP

mFluor Violet 450 SE NB600-844MFV450

mFluor Violet 500 SE NB600-844MFV500

mFluor Violet 610 SE NB600-844MFV610

Catalog# & Formulation	Size	Price
BSA Free NB600-844SS

Azide and BSA Free NBP2-80676

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-844AF350
Alexa Fluor 405	NB600-844AF405
Alexa Fluor 488	NB600-844AF488
Alexa Fluor 532	NB600-844AF532
Alexa Fluor 594	NB600-844AF594
Alexa Fluor 647	NB600-844AF647
Alexa Fluor 700	NB600-844AF700
Alexa Fluor 750	NB600-844AF750
Allophycocyanin	NB600-844APC
Biotin	NB600-844B
CoraFluor 1	NB600-844CL1
DyLight 350	NB600-844UV
DyLight 405	NB600-844V
DyLight 488	NB600-844G
DyLight 550	NB600-844R
DyLight 594	NB600-844DL594
DyLight 650	NB600-844C
DyLight 680	NB600-844FR
DyLight 755	NB600-844IR
FITC	NB600-844F
HRP	NB600-844H
Janelia Fluor 525	NB600-844JF525
Janelia Fluor 549	NB600-844JF549
Janelia Fluor 585	NB600-844JF585
Janelia Fluor 635	NB600-844JF635
Janelia Fluor 646	NB600-844JF646
Janelia Fluor 669	NB600-844JF669
PE	NB600-844PE
PerCP	NB600-844PCP
mFluor Violet 450 SE	NB600-844MFV450
mFluor Violet 500 SE	NB600-844MFV500
mFluor Violet 610 SE	NB600-844MFV610

Collagen II Antibody (5B2.5) - BSA Free

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Collagen II Antibody (5B2.5) - BSA Free Summary

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Publications for Collagen II Antibody (NB600-844)(50)

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Immunogen	A purified preparation of lathyritic type II collagen from embryonic chicken sternum
Localization	Cartilage matrix
Isotype	IgG2a Kappa
Clonality	Monoclonal
Host	Mouse
Gene	COL2A1
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C. Do not freeze.
Buffer	PBS
Preservative	0.02% Sodium Azide
Concentration	1.0 mg/ml
Purity	Protein A purified

Publications using NB600-844	Applications	Species
Zhang W, Wang H, Chu G et al. Mechanically conditioned multilayered angle-ply collagen scaffolds promote annulus fibrosus regeneration Applied Materials Today 2023-04-01 (IHC, Rat)	IHC	Rat
Wang Y, Fan A, Lu L et al. Exosome modification to better alleviates endoplasmic reticulum stress induced chondrocyte apoptosis and osteoarthritis Biochemical pharmacology 2022-11-09 [PMID: 36370754] (IHC-P, Human) Details: Dilution used in IHC-P 1:300	IHC-P	Human
Xu L, Zhang F, Cheng G et al. Attenuation of experimental osteoarthritis with human adipose-derived mesenchymal stem cell therapy: inhibition of the pyroptosis in chondrocytes Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2022-11-04 [PMID: 36331571]
Zhang N, Wang Y, Zhang J Et al. Controlled domain gels with a biomimetic gradient environment for osteochondral tissue regeneration Acta biomaterialia 2021-08-25 [PMID: 34454084] (IHC-P, Rabbit)	IHC-P	Rabbit
Yu H, Liu Y, Yang X et al. Strontium Ranelate Promotes Chondrogenesis Through Inhibition of the Wnt/beta-catenin Pathway Stem Cell Res Ther 2021-05-21 [PMID: 34016181]
He W, Wang W, Deng J et al. L-NMMA AND 1400W, INHIBITORS OF iNOS, ATTENUATE THE INDUCTION OF iNOS AND NO IN PRIMARY RABBIT COSTAL CHONDROCYTES BY FLUORIDE Fluoride 2020-01-01 (WB, Rabbit)	WB	Rabbit

Wu H, Shang Y, Sun W et al. Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl (BSN-GelMA) hydrogel Bioactive materials 2023-01-01 [PMID: 35441114] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Li L, Chen Y, Fu Q Et al. Decellularized extracellular matrix loaded with IPFP-SC for repairing rabbit osteochondral defects American journal of translational research 2021-10-15 [PMID: 34786041] (ICC/IF, Rabbit)	ICC/IF	Rabbit
Wang J, Huang L, Yang X Et al. The Regulatory Effect of MicroRNA-101-3p on Disc Degeneration by the STC1/VEGF/MAPK Pathway Oxidative medicine and cellular longevity 2021-10-05 [PMID: 34650661] (WB, Human)	WB	Human
Zhang W, Wang H, Yuan Z et al. Moderate mechanical stimulation rescues degenerative annulus fibrosus by suppressing caveolin-1 mediated pro-inflammatory signaling pathway International journal of biological sciences 2021-04-03 [PMID: 33867854] (ICC/IF, Rat)	ICC/IF	Rat
Jung H, Mclellan P, Welter JF, Akkus O Chondrogenesis Mesenchymal Stem Cells via Local Release of TGF beta 3 from Heparinized Collagen Biofabric Tissue engineering. Part A 2021-04-07 [PMID: 33827271]
Jing H, Zhang X, Luo K, et al. miR-381-abundant small extracellular vesicles derived from kartogenin-preconditioned mesenchymal stem cells promote chondrogenesis of MSCs by targeting TAOK1 Biomaterials 2020-02-01 [PMID: 31864017] (WB, Human)	WB	Human
Jiang X, Kong Y, Kuss M et al. 3D bioprinting of multilayered scaffolds with spatially differentiated ADMSCs for rotator cuff tendon-to-bone interface regeneration Applied Materials Today 2022-06-01
Yan L, Liu G, Wu X The umbilical cord mesenchymal stem cell-derived exosomal lncRNA H19 improves osteochondral activity through miR-29b-3p/FoxO3 axis Clinical and translational medicine 2021-01-01 [PMID: 33463060] (WB)	WB
Wang H, Xu Y, Wang P, et al. Cell-mediated injectable blend hydrogel-BCP ceramic scaffold for in situ condylar osteochondral repair Acta biomaterialia 2021-01-13 [PMID: 33453407] (IHC-P, Rabbit)	IHC-P	Rabbit
Meng X, Grad S, Wen C et al. An impaired healing model of osteochondral defect in papain-induced arthritis J Orthop Translat 2021-01-13 [PMID: 33437629]
Chen M, Li Y, Liu S et al. Hierarchical macro-microporous WPU-ECM scaffolds combined with Microfracture Promote in Situ Articular Cartilage Regeneration in Rabbits Bioactive Materials 2020-12-21 [PMID: 33426368] (IHC-P, Rabbit)	IHC-P	Rabbit
Wang X, Lu Y, Wang W et al. Effect of different aged cartilage ECM on chondrogenesis of BMSCs in vitro and in vivo Regen Biomater 2020-12-28 [PMID: 33365144]
Qiao Z, Lian M, Han Y et al. Bioinspired stratified electrowritten fiber-reinforced hydrogel constructs with layer-specific induction capacity for functional osteochondral regeneration Biomaterials 2020-10-17 [PMID: 33120203] (WB, Rabbit)	WB	Rabbit
You Q, Liu Z, Zhang J et al. Human Amniotic Mesenchymal Stem Cell Sheets Encapsulating Cartilage Particles Facilitate Repair of Rabbit Osteochondral Defects Am J Sports Med 2020-01-15 [PMID: 31940211]
Yan L, Wu X Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity Cell Biol. Toxicol. 2019-12-09 [PMID: 31820164] (WB, Human)	WB	Human
Tome-Garcia J, Erfani P et al. Analysis of chromatin accessibility uncovers TEAD1 as a regulator of migration in human glioblastoma. Nat Commun 2018-01-10 [PMID: 30275445] (WB, Human)	WB	Human
Dahia CL, Mahoney E, Wylie C. Shh signaling from the nucleus pulposus is required for the postnatal growth and differentiation of the mouse intervertebral disc. PLoS One. 2012-01-01 [PMID: 22558278] (IHC-Fr, ICC/IF, Mouse)	IHC-Fr, ICC/IF	Mouse
Dong Y, Sun X, Zhang Z et al. Regional and sustained dual-release of growth factors from biomimetic tri-layered scaffolds for the repair of large-scale osteochondral defects Applied Materials Today 2020-06-01 (IF/IHC)	IF/IHC
Lee KW, Chung K, Nam DH et al. Decellularized allogeneic cartilage paste with human costal cartilage and crosslinked hyaluronic acid-carboxymethyl cellulose carrier augments microfracture for improved articular cartilage repair Acta biomaterialia 2023-10-07 [PMID: 37813156]
Janssen L, Saranya M, Leinonen M et al. Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation Carbon 2019-11-01 (IF/IHC, Human)	IF/IHC	Human
Luo Z, Liu Z, Yu H et al. Multifunctional mesoporous polydopamine near-infrared photothermal controlled release of kartogenin for cartilage repair Materials & Design 2023-07-01
Zheng Z, Xu J, Tu Z et al. Promoter DNA Hypermethylation of Latexin Promotes Inflammatory Activation of Fibro-Nucleus Pulposus Cells in Intervertebral Disc Degeneration papers.ssrn.com 2023-05-22 (WB, Human)	WB	Human
Saranya M, da Silva AM, Karjalainen H et al. Magnetic-Responsive Carbon Nanotubes Composite Scaffolds for Chondrogenic Tissue Engineering Advanced healthcare materials 2023-09-02 [PMID: 37660271] (IHC, Human)	IHC	Human
Takács R, Vágó J, Póliska S et al. The temporal transcriptomic signature of cartilage formation Nucleic acids research 2023-05-08 [PMID: 36987858] (IHC-P, Chicken) Details: 1:500 IHC-P dilution	IHC-P	Chicken
Wang Y, Zhou Y, Gao X et al. Duhuo Jisheng Decoction Regulates Intracellular Zinc Homeostasis by Enhancing Autophagy via PTEN/Akt/mTOR to Improve Knee Cartilage Degeneration bioRxiv 2023-08-22 (IHC, Rat)	IHC	Rat
Zhang A, Sun L, Chen K et al. 3D printing viscoelastic hydrogel-based scaffolds with a swelling-dependent gate for cartilage injury regeneration Chemical Engineering Journal 2023-11-01 (IHC) Details: Scaffold	IHC
Huang D, Li Y, Ma Z et al. Collagen hydrogel viscoelasticity regulates MSC chondrogenesis in a ROCK-dependent manner Science advances 2023-02-10 [PMID: 36763657] (IHC, Rabbit)	IHC	Rabbit
Wan JT, Qiu XS, Fu ZH et al. Tumor necrosis factor-alpha inhibition restores matrix formation by human adipose-derived stem cells in the late stage of chondrogenic differentiation World Journal of Stem Cells 2022-11-26 [PMID: 36483847] (WB, Human)	WB	Human
V�g� J, Katona �, Tak�cs R et al. Cyclic uniaxial mechanical load enhances chondrogenesis through entraining the molecular circadian clock Journal of Pineal Research 2022-11-01 [PMID: 36030553] (IHC)	IHC
Jun Z, Yuping W, Yanran H et al. Human acellular amniotic membrane scaffolds encapsulating juvenile cartilage fragments accelerate the repair of rabbit osteochondral defects Bone & Joint Research 2022-06-09 [PMID: 35678202]
Jiang X, Wojtkiewicz M, Patwardhan C et al. The effects of maturation and aging on the rotator cuff tendon-to-bone interface The FASEB Journal 2022-03-18 [PMID: 34822203] (B/N)	B/N
Yang Y, Wang X, Zha K et al. Porcine fibrin sealant combined with autologous chondrocytes successfully promotes full-thickness cartilage regeneration in a rabbit model Journal of Tissue Engineering and Regenerative Medicine 2021-09-01 [PMID: 34044473] (IHC)	IHC
Cheng B, Liu Y, Zhao Y et al. The role of anthrax toxin protein receptor 1 as a new mechanosensor molecule and its mechanotransduction in BMSCs under hydrostatic pressure Sci Rep 2019-09-02 [PMID: 31477767] (WB, Rat)	WB	Rat
Suo H, Li L, Zhang C et al. Glucosamine-grafted methacrylated gelatin hydrogels as potential biomaterials for cartilage repair J. Biomed. Mater. Res. Part B Appl. Biomater. 2019-08-01 [PMID: 31369700]
Gao B, Jing H, Gao M et al. Long-segmental Tracheal Reconstruction in Rabbits with Pedicled Tissue-engineered Trachea based on a 3D-printed Scaffold Acta Biomater 2019-07-25 [PMID: 31352107] (IHC-P, Rabbit)	IHC-P	Rabbit
Chen L, Liu G, Li W, Wu X. Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system Cell Mol Biol Lett 2019-12-01 [PMID: 30858866] (WB, Rabbit)	WB	Rabbit
Zhu L, Weng Z, Shen P et al. S100B regulates inflammatory response during osteoarthritis via fibroblast growth factor receptor 1 signaling. Mol Med Rep. 2018-10-01 [PMID: 30280200] (IF/IHC, Human)	IF/IHC	Human
Chen Y, Sui J, Wang Q et al. Injectable self-crosslinking HA-SH/Col I blend hydrogels for in vitro construction of engineered cartilage Carbohydr Polym 2018-06-15 [PMID: 29628260] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Chen Honglin, Malheiro Afonso de Botelho Ferreira Braga, Van Blitterswijk Clemens et al. Direct Writing Electrospinning of Scaffolds with Multidimensional Fiber Architecture for Hierarchical Tissue Engineering. ACS Omega. 2017-11-08 [PMID: 29043781] (IHC-P, Human)	IHC-P	Human
Juhasz T, Szentleleky E, Somogyi CS et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci. 2015-08-03 [PMID: 26230691] (WB, Chicken) Details: Collagen II antibody (clone 5B2.5) was used at 1:100 dilution for WB analysis of lysates from experiments involving chondrifying primary micromass cell cultures that were established from chicken embryos (Ross hybrid) of Hamburger-Hamilton stages 22–24. The cultures were treated or not with Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) PACAP 1–38 and/or transient MS.	WB	Chicken
Lekawanvijit S, Kumfu S, Wang BH et al. The Uremic Toxin Adsorbent AST-120 Abrogates Cardiorenal Injury Following Myocardial Infarction. PLoS One 2013 Dec 13 [PMID: 24349545] (WB, Rat)	WB	Rat
Triulzi T, Ratti M, Tortoreto M et al. Maspin influences response to doxorubicin by changing the tumor microenvironment organization. Int J Cancer. 2013-11-14 [PMID: 24242003] (IHC-P, Human)	IHC-P	Human
Brini AT, Niada S, Lambertini E et al. Chondrogenic potential of Human mesenchymal stem cells and expression of Slug transcription factor. J Tissue Eng Regen Med 2013-07-21 [PMID: 23873763]
Show All 50 Publications. Collapse Publications.

Gene Symbol	COL2A1
Entrez	395069(Chicken) 1280(Human) 12824(Mouse)
Uniprot	Q90W37(Chicken)