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SRY Antibody (1G4) - BSA Free

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Western Blot: SRY Antibody (1G4) [NBP2-37463] - Analysis using SRY mouse mAb against NTERA-2 (1) cell lysate.
Western Blot: SRY Antibody (1G4) [NBP2-37463] - Analysis using SRY mouse mAb against NTERA-2 (1) cell lysate.
Immunohistochemistry-Paraffin: SRY Antibody (1G4) [NBP2-37463] - Analysis of ovarian cancer tissues using SRY mouse mAb with DAB staining.
Flow Cytometry: SRY Antibody (1G4) [NBP2-37463] - Analysis of HepG2 cells using SRY mouse mAb (green) and negative control (red).
Western Blot: SRY Antibody (1G4) [NBP2-37463] - Analysis using SRY mAb against human SRY recombinant protein. (Expected MW is 49.4 kDa)
Immunohistochemistry-Paraffin: SRY Antibody (1G4) [NBP2-37463] - Analysis of cervical cancer tissues using SRY mouse mAb with DAB staining.
ELISA: SRY Antibody (1G4) [NBP2-37463] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
1G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37463-0.025ml
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SRY Antibody (1G4) - BSA Free Summary

Immunogen
Purified recombinant fragment of human SRY (AA:1-204) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SRY
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
23.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SRY Antibody (1G4) - BSA Free

  • essential protein for sex determination in human males
  • sex determining region Y
  • sex-determining region on Y
  • sex-determining region Y protein
  • TDFsex determining region protein
  • TDY
  • Testis-determining factor

Background

SRY encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SRY