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SOX4 Antibody (PCRP-SOX4-1D6)

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Immunocytochemistry/ Immunofluorescence: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SOX4 Antibody (PCRP-SOX4-1D6) followed by goat anti-mouse IgG-CF488 ...read more
Immunohistochemistry-Paraffin: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with SOX4 antibody (PCRP-SOX4-1D6). HIER: Tris/EDTA, pH9.0, 45min. ...read more
Flow Cytometry: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Flow Cytometric Analysis of PFA-fixed HeLa cells. SOX4 antibody (PCRP-SOX4-1D6) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Immunohistochemistry-Paraffin: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with SOX4 antibody (PCRP-SOX4-1D6). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, ...read more
Protein Array: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX4 Antibody (PCRP-SOX4-1D6).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, MA
Clone
PCRP-SOX4-1D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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SOX4 Antibody (PCRP-SOX4-1D6) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14180)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant fragment (around aa45-130) of human SOX4 protein (Uniprot: Q06945)
Localization
Nucleus
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SOX4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react in Xenopus and Chicken.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for SOX4 Antibody (PCRP-SOX4-1D6)

  • ecotropic viral integration site 16
  • EVI16
  • SRY (sex determining region Y)-box 4
  • SRY-related HMG-box gene 4
  • transcription factor SOX-4

Background

SOX4 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX4 Antibody (NBP3-13777) (0)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SOX4 Antibody (NBP3-13777) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on SOX4.

Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling
By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric ...  Read full blog post.

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Bioinformatics

Gene Symbol SOX4
Uniprot