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SOX4 Antibody (PCRP-SOX4-1D6)

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Immunocytochemistry/Immunofluorescence: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SOX4 Antibody (PCRP-SOX4-1D6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunocytochemistry/ Immunofluorescence: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SOX4 Antibody (PCRP-SOX4-1D6) followed by goat anti-mouse IgG-CF488 ...read more
Immunohistochemistry-Paraffin: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with SOX4 antibody (PCRP-SOX4-1D6). HIER: Tris/EDTA, pH9.0, 45min. ...read more
Flow Cytometry: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Flow Cytometric Analysis of PFA-fixed HeLa cells. SOX4 antibody (PCRP-SOX4-1D6) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Immunohistochemistry-Paraffin: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with SOX4 antibody (PCRP-SOX4-1D6). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, ...read more
Protein Array: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX4 Antibody (PCRP-SOX4-1D6).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, MA
Clone
PCRP-SOX4-1D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-14180AF350
Alexa Fluor 405
NBP3-14180AF405
Alexa Fluor 488
NBP3-14180AF488
Alexa Fluor 532
NBP3-14180AF532
Alexa Fluor 594
NBP3-14180AF594
Alexa Fluor 647
NBP3-14180AF647
Alexa Fluor 700
NBP3-14180AF700
Alexa Fluor 750
NBP3-14180AF750
Allophycocyanin
NBP3-14180APC
Biotin
NBP3-14180B
CoraFluor 1
NBP3-14180CL1
DyLight 350
NBP3-14180UV
DyLight 405
NBP3-14180V
DyLight 488
NBP3-14180G
DyLight 550
NBP3-14180R
DyLight 594
NBP3-14180DL594
DyLight 650
NBP3-14180C
DyLight 680
NBP3-14180FR
DyLight 755
NBP3-14180IR
FITC
NBP3-14180F
HRP
NBP3-14180H
Janelia Fluor 525
NBP3-14180JF525
Janelia Fluor 549
NBP3-14180JF549
Janelia Fluor 585
NBP3-14180JF585
Janelia Fluor 635
NBP3-14180JF635
Janelia Fluor 646
NBP3-14180JF646
Janelia Fluor 669
NBP3-14180JF669
PE
NBP3-14180PE
PerCP
NBP3-14180PCP
mFluor Violet 450 SE
NBP3-14180MFV450
mFluor Violet 500 SE
NBP3-14180MFV500
mFluor Violet 610 SE
NBP3-14180MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-14180
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SOX4 Antibody (PCRP-SOX4-1D6) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14180)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant fragment (around aa45-130) of human SOX4 protein (Uniprot: Q06945)
Localization
Nucleus
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SOX4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react in Xenopus and Chicken.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for SOX4 Antibody (PCRP-SOX4-1D6)

  • ecotropic viral integration site 16
  • EVI16
  • SRY (sex determining region Y)-box 4
  • SRY-related HMG-box gene 4
  • transcription factor SOX-4

Background

SOX4 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SOX4 Antibody
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NBP3-13777
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SOX4
Uniprot