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SOX17 Antibody [Unconjugated]

Images

 
Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody ...read more
Endoderm-differentiated D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX17/DNA complexes were immunoprecipitated using 5 µg Goat ...read more
SOX17 was detected in immersion fixed B16 mouse melanoma cell line using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were ...read more
SOX17 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room ...read more
Simple Western lane view shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 59 kDa (as indicated) ...read more
Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately ...read more
Western blot shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line, OVCAR‑3 human ovarian carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human SOX17 Antigen ...read more
Generation and characterization of systemic lupus erythematosus (SLE)‐specific human‐induced pluripotent stem cells (hiPSCs). Immunostaining of Otx2 (ectoderm marker), Sox17 (endoderm marker), Brachyury (mesoderm ...read more
Concordant expression of developmental programs across organoids from human iPSC lines. IF staining of D29 kidney organoid (AS line, ML protocol) for SOX17 and CD31, markers of endothelial cells. Image collected and ...read more
Activin-A levels during Day 0–1 modulate cardiomyocyte (CM) vs. definitive endoderm (DE) differentiation. Pluripotent H1 ESCs were induced by changing medium to RPMI/B27 (no insulin), including CHIR (7.5 μmol/L) ...read more
In vitro and in-TiPSCs. Immunofluorescence staining for Sox17 (endodermal marker), alpha SMA (mesodermal marker), and Nestin (ectodermal marker) in each human TiPSCs1-derived differentiated cell in vitro. Image ...read more
Differentiation of hESCs into definitive endoderm cells by Activin A with CHIR99021. Immunofluorescence analysis of the expression of SOX17 and FoxA2 for Activin A with 2 µM CHIR99021-induced differentiated cells on ...read more
HIF-1 alpha activates transcription of Sox17. Western blot analysis in control human lung microvascular endothelial cells (HLMVECs) and HLMVECs for which CRISPR/Cas9 was used to delete HIFs. ECs were treated with the ...read more
Features of ESC-INTPSCs intermediate between ESCs and EpiSCs. Expression of OCT3/4 and SOX17. Scale bar, 100 µm. Image collected and cropped by CiteAb from the following publication ...read more
p38-Mapk14/11 inhibition during murine blastocyst maturation blocks primitive endoderm (PrE) differentiation/maturation. Representative single confocal z-plane micrographs of vehicle control-treated (+DMSO) or ...read more
Endothelial cell (EC) specific deletion of Sox17 (Sox17EC−/−) in mice prevents endothelial regeneration. Western blot analysis of Sox17 protein expression in isolated ECs obtained from flushed lungs of ...read more
Characterization of hybrid cells.(A) Both ES-pNSC and pES-NSC hybrid cells are positive for alkaline phosphatase staining (100 ×). (B) RT-PCR analysis of Oct4, Nanog, Sox2, and Nestin expression in fusion partner and ...read more
In vitro and in-TiPSCs.(A): Immunofluorescence staining for Sox17 (endodermal marker), alpha SMA (mesodermal marker), and Nestin (ectodermal marker) in each TiPSCs1-derived differentiated cell in vitro. (B): Gross ...read more
Characterization of hybrid cells.(A) Both ES-pNSC and pES-NSC hybrid cells are positive for alkaline phosphatase staining (100 ×). (B) RT-PCR analysis of Oct4, Nanog, Sox2, and Nestin expression in fusion partner and ...read more
Activation of Sox17 at onset of EC regeneration and Sox17-mediated Cyclin E1 expression. a qPCR analysis of gene expression in sorted CD31+ cells from mTmG-Scl mice before and after injury (12 mg/kg i.p.). Sox17, ...read more
Generation and characterization of systemic lupus erythematosus (SLE)‐specific human‐induced pluripotent stem cells (hiPSCs). (A) Dermal fibroblasts derived from patient with SLE were reprogrammed into iPSCs using ...read more
soHIF-1 alpha signaling induces Sox17 expression. a MPO activity of flushed lung sample from mice challenged with LPS (12 mg/kg i.p.) for 6 and 24 h. n = 3. b Western blot analysis of wild-type mice lung before ...read more
PGCLCs derived from hc-iPSCs.Immunofluorescent staining of (A) BLIMP1 (green color) and (B) OCT4 (green color) in 4-day-old EBs cultured with PGC induction medium on hc-iPSC-1, -2, -5 and -6 (third to fifth column), ...read more
Activin-A levels during Day 0–1 modulate CM vs. DE differentiation.Pluripotent H1 ESCs were induced by changing medium to RPMI/B27 (no insulin), including CHIR (7.5 μmol/L) during Day 0–1 and IWP (5 μmol/L) during ...read more
Features of ESC-INTPSCs intermediate between ESCs and EpiSCs.(A) Comparison of gene expression in ESCs, EpiSCs, and ESC-INTPSCs. (B) The number of AP-positive colonies formed in the LIF/2i condition four days after 1000 ...read more
Activation of Sox17 at onset of EC regeneration and Sox17-mediated Cyclin E1 expression. a qPCR analysis of gene expression in sorted CD31+ cells from mTmG-Scl mice before and after injury (12 mg/kg i.p.). Sox17, ...read more
PGCLCs derived from hc-iPSCs.Immunofluorescent staining of (A) BLIMP1 (green color) and (B) OCT4 (green color) in 4-day-old EBs cultured with PGC induction medium on hc-iPSC-1, -2, -5 and -6 (third to fifth column), ...read more
soHIF-1 alpha signaling induces Sox17 expression. a MPO activity of flushed lung sample from mice challenged with LPS (12 mg/kg i.p.) for 6 and 24 h. n = 3. b Western blot analysis of wild-type mice lung before ...read more
EC specific deletion of Sox17 (Sox17EC−/−) in mice prevents endothelial regeneration. a Schematic diagram of Sox17fl/fl mice crossed with Scl-CreERT2 to delete Sox17 in ECs. After tamoxifen feeding for five days and ...read more
p38-Mapk14/11 inhibition during blastocyst maturation blocks PrE differentiation/maturation. (a) Experimental schema of p38-Mapk14/11 inhibition (+SB220025), plus vehicle control (+DMSO), and the details of antibodies ...read more
Differentiation of hESCs into definitive endoderm cells by Activin A with CHIR99021.a The relative primitive streak (BRACHYRUY, MESP1, and MIXL1) gene expression of the day 1 differentiated cells by adding Activin A ...read more
HIF-1 alpha activates transcription of Sox17. a Western blot analysis in control HLMVECs and HLMVECs for which CRISPR/Cas9 was used to delete HIFs. ECs were treated with the HIF prolyl hydroxylase inhibitor DMOG to ...read more
Concordant expression of developmental programs across organoids from four human iPSC lines. a Heatmap of expression patterns for major nephrogenesis markers across organoid differentiation time points (iPSC D0, D7, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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SOX17 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2
Specificity
Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX18 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX17
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1-2 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF1924 in the following applications:

Publications
Read Publications using
AF1924 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX17 Antibody [Unconjugated]

  • FLJ22252
  • SOX17
  • SRY (sex determining region Y)-box 17
  • SRY-related HMG-box transcription factor SOX17
  • transcription factor SOX-17
  • VUR3

Background

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).

  1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
  2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX17 Antibody (AF1924)(451)

We have publications tested in 11 confirmed species: Human, Mouse, Rat, Bovine, Callithrix jacchus (Marmoset), Canine, Marmoset, Pan troglodytes (Chimpanzee), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Transgenic Mouse.

We have publications tested in 12 applications: Bioassay, Flow Cytometry, Functional Assay, ICC, IF, IHC, IHC-Fr, IHC-P, IHC/IF, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
Flow Cytometry
(41)
Functional Assay
(1)
ICC
(200)
IF
(3)
IHC
(76)
IHC-Fr
(6)
IHC-P
(2)
IHC/IF
(1)
Immunoprecipitation
(1)
Neutralization
(1)
Western Blot
(14)
All Applications
Filter By Species
Human
(228)
Mouse
(74)
Rat
(1)
Bovine
(1)
Callithrix jacchus (Marmoset)
(2)
Canine
(1)
Marmoset
(1)
Pan troglodytes (Chimpanzee)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 451. Show All 451 Publications.
Publications using AF1924 Applications Species
Cukier, HN;Simon, SA;Tang, E;Golightly, CG;Laverde-Paz, MJ;Adams, LD;Starks, TD;Vance, JM;Cuccaro, ML;Haines, JL;Byrd, GS;Pericak-Vance, MA;Dykxhoorn, DM; Generation of an induced pluripotent stem cell line (UMi043-A) from an African American patient with Alzheimer's disease carrying an ABCA7 deletion (p.Arg578Alafs) Stem cell research 2024-02-25 [PMID: 38422817] (Human) Human
Kruger, RE;Frum, T;Brumm, AS;Hickey, SL;Niakan, KK;Aziz, F;Shammami, MA;Roberts, JG;Ralston, A; Smad4 is essential for epiblast scaling and morphogenesis after implantation, but nonessential prior to implantation in the mouse bioRxiv : the preprint server for biology 2024-01-24 [PMID: 38328075] (IHC, Mouse) IHC Mouse
Daya, NM;Döring, K;Zhuge, H;Volke, L;Stab, V;Dietz, J;Athamneh, M;Roos, A;Zaehres, H;Güttsches, AK;Mavrommatis, L;Vorgerd, M; Generation of two hiPSCs lines of two patients carrying truncating mutations in the dimerization domain of filamin C Stem cell research 2024-01-27 [PMID: 38309149] (Flow Cytometry, ICC, Human) Flow Cytometry, ICC Human
Tripathi, D;Manhas, A;Noishiki, C;Wu, D;Adkar, S;Sallam, K;Fukaya, E;Leeper, NJ;Sayed, N; Generation of induced pluripotent stem cell line from a patient suffering from arterial calcification due to deficiency of CD73 (ACDC) Stem cell research 2023-12-19 [PMID: 38199067] (IHC, Human) IHC Human
Lu, X;Luo, Y;Nie, X;Zhang, B;Wang, X;Li, R;Liu, G;Zhou, Q;Liu, Z;Fan, L;Hotaling, JM;Zhang, Z;Bo, H;Guo, J; Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment Nature communications 2023-12-20 [PMID: 38123589] (IHC, Human) IHC Human
Driver, K;Vo, C;Scriba, CK;Saker, S;Larmonier, T;Malfatti, E;Romero, NB;Ravenscroft, G;Laing, NG;Taylor, RL;Clayton, JS; Generation of two induced pluripotent stem cell lines from a 33-year-old central core disease patient with a heterozygous dominant c.14145_14156delCTACTGGGACA (p.Asn4715_Asp4718del) deletion in the RYR1 gene Stem cell research 2023-11-22 [PMID: 38029555] (ICC, Human) ICC Human
Boeing, A;Mavrommatis, L;Daya, NM;Zhuge, H;Volke, L;Kocabas, A;Kneifel, M;Athamneh, M;Krause, K;Südkamp, N;Döring, K;Theiss, C;Roos, A;Zaehres, H;Güttsches, AK;Vorgerd, M; Generation of two human iPSC lines (HIMRi002-A and HIMRi003-A) derived from Caveolinopathy patients with rippling muscle disease Stem cell research 2023-10-04 [PMID: 37839261] (ICC, Human) ICC Human
Isaac, E;Berg, DK;Pfeffer, PL; Using extended growth of cattle embryos in culture to gain insights into bovine developmental events on embryonic days 8 to 10 Theriogenology 2023-10-06 [PMID: 37837723] (IHC, Bovine) IHC Bovine
Lyu, X;Rowley, MJ;Kulik, MJ;Dalton, S;Corces, VG; Regulation of CTCF loop formation during pancreatic cell differentiation Nature communications 2023-10-09 [PMID: 37813869] (ICC, Human) ICC Human
Yohko Kitagawa, Akihiro Ikenaka, Ryohichi Sugimura, Akira Niwa, Megumu K. Saito ZEB2 and MEIS1 independently contribute to hematopoiesis via early hematopoietic enhancer activation iScience 10/20/2023 [PMID: 37771659]
Show All 451 Publications.

Reviews for SOX17 Antibody (AF1924) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Other.

Reviews using AF1924:
Filter by Applications
IF
(3)
All Applications
Filter by Species
Human
(2)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IF Human 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMIC 23658023
SpeciesHuman
  5
reviewed by:
Verified Customer
IF Human 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMIC 22613812
SpeciesHuman
  4
reviewed by:
Verified Customer
IF Other 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMIC 23418163
SpeciesOther

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Recent Reviews

4.7
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Verified Customer
01/05/2015
Application: IF
Species: Human

 
Verified Customer
01/05/2015
Application: IF
Species: Human

 
Verified Customer
01/05/2015
Application: IF
Species: Other

Bioinformatics

Gene Symbol SOX17
Uniprot