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SOX21 Antibody [Unconjugated]

 (6 Publications)
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SOX21 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Goat Anti-Human SOX21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3538) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
SOX21 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Goat Anti-Human SOX21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3538) at 10 µg/mL for 3 hours at room ...read more
SOX21 was detected in immersion fixed paraffin-embedded sections of human glioblastoma using Goat Anti-Human SOX21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3538) at 0.5 µg/ml for 1 hour at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF3538
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3538-SP
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SOX21 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX21
Ser2-Leu276 (Gly102Ser)
Accession # Q9Y651
Specificity
Detects human SOX21 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX1, rhSOX2 and rhSOX15 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX21
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF3538 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX21 Antibody [Unconjugated]

  • SOX21
  • SOX25
  • SOX25SOX-A
  • SOXA
  • SRY (sex determining region Y)-box 21
  • SRY-box 21
  • transcription factor SOX-21

Background

SOX21 is a member of the B group of the SOX family HMG-box transcription factors. It is a 276 amino acid residue protein that has an N-terminal HMG-box and a C‑terminal domain that is required for the SOX21 neurogenesis function. Human and mouse SOX 21 share 99% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX21 Antibody (AF3538)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IF, Immunoprecipitation.


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IF
(1)
Immunoprecipitation
(1)
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(1)
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(1)
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Showing Publications 1 - 6 of 6.
Publications using AF3538 Applications Species
Evelien Eenjes, Floor Benthem, Anne Boerema-de Munck, Marjon Buscop-van Kempen, Dick Tibboel, Robbert J. Rottier Distinct roles for SOX2 and SOX21 in differentiation, distribution and maturation of pulmonary neuroendocrine cells Cellular and Molecular Life Sciences 3/3/2023 [PMID: 36867267]
Evelien Eenjes, Marjon Buscop-van Kempen, Anne Boerema-de Munck, Gabriela G Edel, Floor Benthem, Lisette de Kreij-de Bruin et al. SOX21 modulates SOX2-initiated differentiation of epithelial cells in the extrapulmonary airways eLife 7/21/2021 [PMID: 34286693]
N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates Cell Rep, 2020-05-05;31(5):107599. 2020-05-05 [PMID: 32375049] (IF, Human) IF Human
Leonard Y. M. Cheung, Hideyuki Okano, Sally A. Camper Sox21 deletion in mice causes postnatal growth deficiency without physiological disruption of hypothalamic-pituitary endocrine axes Molecular and Cellular Endocrinology 1/1/2017 [PMID: 27616671]
Mateo J, van den Berg D, Haeussler M, Drechsel D, Gaber Z, Castro D, Robson P, Crawford G, Flicek P, Ettwiller L, Wittbrodt J, Guillemot F, Martynoga B Characterization of the neural stem cell gene regulatory network identifies OLIG2 as a multifunctional regulator of self-renewal. Genome Res, 2014-10-07;25(1):41-56. 2014-10-07 [PMID: 25294244] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Satoru Matsuda, Ken-ichiro Kuwako, Hirotaka James Okano, Shuichi Tsutsumi, Hiroyuki Aburatani, Yumiko Saga et al. Sox21 promotes hippocampal adult neurogenesis via the transcriptional repression of the Hes5 gene The Journal of Neuroscience 9/5/2012 [PMID: 22956844]

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Product General Protocols

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Endoderm-differentiated D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX17/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) or control antibody (AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. Thep21promoter was detected by standard PCR.
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Western blot shows lysates of D3 mouse embryonic stem cell line, NTera-2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Bioinformatics

Gene Symbol SOX21
Uniprot