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SOX1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of undifferentiated iBJ6 human iPS cells and iBJ6 human iPS cells differentiated into neuroprogenitor cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 Antigen ...read more
SOX1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neural progenitor cells using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at ...read more
SOX1 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiated with retinoic acid using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
SOX1 was detected in immersion fixed rat cortical stem cells (NSC001) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. ...read more
SOX1 was detected in immersion fixed mouse cortical stem cells (Catalog # NSC002) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room ...read more
SOX1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 3 µg/mL for 1 ...read more
Simple Western lane view shows lysates of undifferentiated iBJ6 human iPS cells andiBJ6 human iPS cells differentiated into neuroprogenitor cells, loaded at 0.2 mg/mL. A specific band was detected for SOX1 at ...read more
Characterization of NSCs and cortical neurons derived from FKRP‐ and CRISPR/Cas9 corrected‐iPSCsA, BRepresentative images of NSCs derived from FKRP‐ and corrected‐iPSC lines expressing SOX1, SOX2, and nestin.C, ...read more
Characterization of NSCs and cortical neurons derived from FKRP‐ and CRISPR/Cas9 corrected‐iPSCsA, BRepresentative images of NSCs derived from FKRP‐ and corrected‐iPSC lines expressing SOX1, SOX2, and nestin.C, ...read more
Expression of NSUN2 in the Human Developing Brain and NES Cells(A) DAPI-stained human embryo (6 weeks of gestation) marked for prosencephalon, mesencephalon, and rhombencephalon. Region in square is magnified in (B). ...read more
Expression of NSUN2 in the Human Developing Brain and NES Cells(A) DAPI-stained human embryo (6 weeks of gestation) marked for prosencephalon, mesencephalon, and rhombencephalon. Region in square is magnified in (B). ...read more
GPI anchored proteins are required for neural differentiation.(A). Representative example of images of hiPSC-derived EBs and EB-derived rosettes during neural differentiation. Neural induction and rosette formation upon ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

SOX1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX1
Asn242-Gly379 (Leu276Ile)
Accession # NP_005977
Specificity
Detects human SOX1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3369 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX1 Antibody [Unconjugated]

  • SOX1
  • SRY (sex determining region Y)-box 1
  • SRY-related HMG-box gene 1
  • transcription factor SOX-1

Background

SOX1 is a 39 kDa transcription factor that belongs to the SOXB1 subgroup. Within the developing CNS, SOX1 maintains neural cells in an undifferentiated state and has been used as a marker for neural stem cells. Human and mouse SOX1 share 97% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX1 Antibody (AF3369)(126)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Primate (Macaca fascicularis), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 7 applications: Bioassay, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Bioassay
(1)
Flow Cytometry
(1)
ICC
(51)
IHC
(20)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(57)
Mouse
(15)
Rat
(1)
Primate (Macaca fascicularis)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 126. Show All 126 Publications.
Publications using AF3369 Applications Species
Capauto, D;Wang, Y;Wu, F;Norton, S;Mariani, J;Inoue, F;Crawford, GE;Ahituv, N;Abyzov, A;Vaccarino, FM; Characterization of enhancer activity in early human neurodevelopment using Massively Parallel Reporter Assay (MPRA) and forebrain organoids Scientific reports 2024-02-16 [PMID: 38365907] (ICC, Human) ICC Human
Liu, C;Xie, Y;Chen, X;Liu, L;Liu, C;Yin, Z; BAF45D-binding to HOX genes was differentially targeted in H9-derived spinal cord neural stem cells Scientific reports 2024-01-02 [PMID: 38168763] (ICC, Human) ICC Human
Tao, Y;Li, X;Dong, Q;Kong, L;Petersen, AJ;Yan, Y;Xu, K;Zima, S;Li, Y;Schmidt, DK;Ayala, M;Mathivanan, S;Sousa, AMM;Chang, Q;Zhang, SC; Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells Nature biotechnology 2023-11-16 [PMID: 37974010] (Flow Cytometry, IHC, Mouse) Flow Cytometry, IHC Mouse
Jaratsittisin, J;Sornjai, W;Chailangkarn, T;Jongkaewwattana, A;Smith, DR; The vitamin D receptor agonist EB1089 can exert its antiviral activity independently of the vitamin D receptor PloS one 2023-10-17 [PMID: 37847693] (ICC, Human) ICC Human
Oleksy, C;Massart, F;Goldwurm, S;Arado, A;Arena, G;Boussaad, I;Krüger, R; Generation and characterization of induced pluripotent stem cells from a Parkinson's disease patient carrying the digenic LRRK2 p.G2019S and GBA1 p.N409S mutations Stem cell research 2023-09-28 [PMID: 37832355] (ICC, Human) ICC Human
Fiock, KL;Hook, JN;Hefti, MM; Determinants of astrocytic pathology in stem cell models of primary tauopathies Acta neuropathologica communications 2023-10-06 [PMID: 37803326] (Bioassay, Human) Bioassay Human
Stanberry, I;Cunningham, D;Ye, S;Alonzo, M;Zhao, MT;Garg, V;Lilly, B; Characterization of an induced pluripotent stem cell line NCHi011-A from a 23-year-old female with Alagille Syndrome harboring a heterozygous JAG1 pathogenic variant Stem cell research 2023-09-23 [PMID: 37774637] (ICC, Human) ICC Human
Agnes Lee Chen Ong, Toshiya Kokaji, Arisa Kishi, Yoshihiro Takihara, Takuma Shinozuka, Ren Shimamoto et al. Acquisition of neural fate by combination of BMP blockade and chromatin modification iScience 10/20/2023 [PMID: 37771660]
Shirley D. Wenker, María Isabel Farias, Victoria Gradaschi, Corina Garcia, Juan Beauquis, María Celeste Leal et al. Microglia-secreted TNF-alpha affects differentiation efficiency and viability of pluripotent stem cell-derived human dopaminergic precursors PLoS One 9/26/2023 [PMID: 37751438]
Patrycja Mulica, Carmen Venegas, Zied Landoulsi, Katja Badanjak, Sylvie Delcambre, Maria Tziortziou et al. Comparison of two protocols for the generation of iPSC-derived human astrocytes Biol Proced Online 9/20/2023 [PMID: 37730545]
Show All 126 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Blogs on SOX1.

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By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

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Bioinformatics

Gene Symbol SOX1
Uniprot