I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?

Name: Smad3 [p Ser425, p Ser423] Antibody [CoraFluor&#8482; 1]
Brand: Novus Biologicals
SKU: NBP1-77836CL1
Price: 1019 USD

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Accepted Answer

We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, MuSpecies Glossary
Applications	WB, ELISA, Flow, ICC/IF, IHC, Single-Cell Western
Clonality	Polyclonal
Host	Rabbit
Conjugate	CoraFluor 1

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP1-77836AF350

Alexa Fluor 405 NBP1-77836AF405

Alexa Fluor 488 NBP1-77836AF488

Alexa Fluor 532 NBP1-77836AF532

Alexa Fluor 594 NBP1-77836AF594

Alexa Fluor 647 NBP1-77836AF647

Alexa Fluor 700 NBP1-77836AF700

Alexa Fluor 750 NBP1-77836AF750

Allophycocyanin NBP1-77836APC

Biotin NBP1-77836B

DyLight 350 NBP1-77836UV

DyLight 405 NBP1-77836V

DyLight 488 NBP1-77836G

DyLight 550 NBP1-77836R

DyLight 594 NBP1-77836DL594

DyLight 650 NBP1-77836C

DyLight 680 NBP1-77836FR

DyLight 755 NBP1-77836IR

FITC NBP1-77836F

HRP NBP1-77836H

Janelia Fluor 525 NBP1-77836JF525

Janelia Fluor 549 NBP1-77836JF549

Janelia Fluor 646 NBP1-77836JF646

Janelia Fluor 669 NBP1-77836JF669

PE NBP1-77836PE

PerCP NBP1-77836PCP

Unconjugated NBP1-77836

mFluor Violet 450 SE NBP1-77836MFV450

mFluor Violet 500 SE NBP1-77836MFV500

mFluor Violet 610 SE NBP1-77836MFV610

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77836AF350
Alexa Fluor 405	NBP1-77836AF405
Alexa Fluor 488	NBP1-77836AF488
Alexa Fluor 532	NBP1-77836AF532
Alexa Fluor 594	NBP1-77836AF594
Alexa Fluor 647	NBP1-77836AF647
Alexa Fluor 700	NBP1-77836AF700
Alexa Fluor 750	NBP1-77836AF750
Allophycocyanin	NBP1-77836APC
Biotin	NBP1-77836B
DyLight 350	NBP1-77836UV
DyLight 405	NBP1-77836V
DyLight 488	NBP1-77836G
DyLight 550	NBP1-77836R
DyLight 594	NBP1-77836DL594
DyLight 650	NBP1-77836C
DyLight 680	NBP1-77836FR
DyLight 755	NBP1-77836IR
FITC	NBP1-77836F
HRP	NBP1-77836H
Janelia Fluor 525	NBP1-77836JF525
Janelia Fluor 549	NBP1-77836JF549
Janelia Fluor 646	NBP1-77836JF646
Janelia Fluor 669	NBP1-77836JF669
PE	NBP1-77836PE
PerCP	NBP1-77836PCP
Unconjugated	NBP1-77836
mFluor Violet 450 SE	NBP1-77836MFV450
mFluor Violet 500 SE	NBP1-77836MFV500
mFluor Violet 610 SE	NBP1-77836MFV610

Description	CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen	Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)
Modification	p Ser425, p Ser423
Specificity	This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	SMAD3
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Smad3 [p Ser425, p Ser423] Antibody [CoraFluor™ 1]

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Dilutions	ELISA Flow Cytometry Immunocytochemistry/ Immunofluorescence Immunohistochemistry Immunohistochemistry-Paraffin Single Cell Western Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark. Do not freeze.
Buffer	PBS
Preservative	No Preservative
Purity	Immunogen affinity purified