![Western Blot: SETDB1 Antibody [NBP2-20322] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SETDB1-Antibody-Western-Blot-NBP2-20322-img0009.jpg "Western Blot: SETDB1 Antibody [NBP2-20322] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, Func, ICC/IF, IHC, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human SETDB1. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Rat (90%), Bovine (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SETDB1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Theoretical MW | 143 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Reviewed Applications |
|
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Immunohistochemistry-Paraffin KAP1 [p Ser824] Antibody](https://images.novusbio.com/images/KAP1-[p-Ser824]-Antibody-Immunohistochemistry-Paraffin-NB100-2350-img0013.jpg)
![Immunohistochemistry-Paraffin KAP1 [p Ser824] Antibody](https://images.novusbio.com/images/KAP1-[p-Ser824]-Antibody-Immunohistochemistry-Paraffin-NB100-2350-img0014.jpg)
![Immunocytochemistry/ Immunofluorescence KAP1 [p Ser824] Antibody](https://images.novusbio.com/images/KAP1-[p-Ser824]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2350-img0015.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
WB | Human | 06/18/2018 |
Summary
|
||||||||
Enlarge |
reviewed by:
Verified Customer |
ICC | Human | 07/12/2017 |
Summary
|
||||||||
|
reviewed by:
Verified Customer |
IP | Human | 04/20/2017 |
Summary
|
|||||||||
Enlarge |
reviewed by:
Verified Customer |
WB | Human | 04/19/2017 |
Summary
|
Secondary Antibodies |
Isotype Controls |
Research Areas for SETDB1 Antibody (NBP2-20322)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
5 | |
4 | |
3 | |
2 | |
1 |
| Verified Customer 06/18/2018 |
||
| Application: | WB | |
| Species: | Human |
| Verified Customer 07/12/2017 |
||
| Application: | ICC | |
| Species: | Human |
| Verified Customer 04/20/2017 |
||
| Application: | IP | |
| Species: | Human |
![Immunocytochemistry: SETDB1 Antibody [NBP2-20322] - Paraformaldehyde Fixed Human Dermal Microvascular Endothelial Cells were immunostained with 1:100 dilution of pAb. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SETDB1-Antibody-Immunocytochemistry-NBP2-20322-img0006.jpg "Immunocytochemistry: SETDB1 Antibody [NBP2-20322] - Paraformaldehyde Fixed Human Dermal Microvascular Endothelial Cells were immunostained with 1:100 dilution of pAb. Image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: SETDB1 Antibody [NBP2-20322] - Mouse testis. SETDB1 stained by SETDB1 antibody [N2C1], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/SETDB1-Antibody-Immunohistochemistry-Paraffin-NBP2-20322-img0007.jpg "Immunohistochemistry-Paraffin: SETDB1 Antibody [NBP2-20322] - Mouse testis. SETDB1 stained by SETDB1 antibody [N2C1], Internal diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Chromatin Immunoprecipitation (ChIP): SETDB1 Antibody [NBP2-20322] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-SETDB1 antibody.](http://images.novusbio.com/fullsize/SETDB1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-20322-img0003.jpg "Chromatin Immunoprecipitation (ChIP): SETDB1 Antibody [NBP2-20322] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-SETDB1 antibody.")
![Immunoprecipitation: SETDB1 Antibody [NBP2-20322] - HeLa nuclear extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 ug SETDB1 antibody [N2C1], Internal 5 % SDS-PAGE The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N2C1], Internal diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/SETDB1-Antibody-Immunoprecipitation-NBP2-20322-img0005.jpg "Immunoprecipitation: SETDB1 Antibody [NBP2-20322] - HeLa nuclear extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 ug SETDB1 antibody [N2C1], Internal 5 % SDS-PAGE The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N2C1], Internal diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]")
![ICC/IF-SETDB1 Antibody [NBP2-20322] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SETDB1 stained by SETDB1 antibody [N2C1], Internal diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/antibody/nbp2-20322_rabbit-polyclonal-setdb1-antibody-immunocytochemistry-immunofluorescence-8122023143536..jpg "ICC/IF-SETDB1 Antibody [NBP2-20322] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SETDB1 stained by SETDB1 antibody [N2C1], Internal diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Scale bar= 10um.")
![Western Blot G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-4120241252118.jpg)
![Immunohistochemistry G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-412024125214.jpg)
![Immunohistochemistry G9a/EHMT2 Antibody (A8620A) [Unconjugated]](https://images.novusbio.com/images/pp-a8620a-00_human-mouse-g9a-ehmt2-mab-clone-a8620a-4120241251265.jpg)
![N/A alpha-Defensin 1 [Biotin]](https://images.novusbio.com/images2/DATA_alphaDefensin_1_DY819805_ELISA_2534.jpg)
![GLP/EHMT1 Antibody (B0422) [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "GLP/EHMT1 Antibody (B0422) [Unconjugated]")
![Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Chromatin_Immunoprecipitation_11218.jpg)
![Immunocytochemistry Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Immunocytochemistry_7093.jpg)
![Simple Western Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct3_4_AF1759_Simple_Western_20183.jpg)
![Western Blot CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/CBP_MAB2676_Western_Blot_6043.jpg)
![Immunocytochemistry CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/mab2676_human-mouse-rat-cbp-mab-clone-257003-immunocytochemistry-101220208237.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
