![Western Blot: NLK Antibody [NBP1-32264] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NLK antibody diluted at 1:500.](http://images.novusbio.com/fullsize/NLK-Antibody-Western-Blot-NBP1-32264-img0006.jpg "Western Blot: NLK Antibody [NBP1-32264] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NLK antibody diluted at 1:500.")
| Reactivity | Hu, Mu, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NLK. The exact sequence is proprietary. |
| Localization | Nucleus, Cytoplasm |
| Predicted Species | Xenopus (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NLK |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 58 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for NLK Antibody (NBP1-32264)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NLK |
![Immunocytochemistry/Immunofluorescence: NLK Antibody [NBP1-32264] - Paraformaldehyde-fixed HeLa, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/NLK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32264-img0002.jpg "Immunocytochemistry/Immunofluorescence: NLK Antibody [NBP1-32264] - Paraformaldehyde-fixed HeLa, using antibody at 1:100 dilution.")
![Immunohistochemistry-Paraffin: NLK Antibody [NBP1-32264] - Paraffin-embedded mouse ovary. NLK antibody [N3C3] ) dilution: 1:500.](http://images.novusbio.com/fullsize/NLK-Antibody-Immunohistochemistry-Paraffin-NBP1-32264-img0005.jpg "Immunohistochemistry-Paraffin: NLK Antibody [NBP1-32264] - Paraffin-embedded mouse ovary. NLK antibody [N3C3] ) dilution: 1:500.")
![Western Blot: NLK Antibody [NBP1-32264] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NLK-Antibody-Western-Blot-NBP1-32264-img0003.jpg "Western Blot: NLK Antibody [NBP1-32264] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: NLK Antibody [NBP1-32264] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE NLK antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NLK-Antibody-Western-Blot-NBP1-32264-img0004.jpg "Western Blot: NLK Antibody [NBP1-32264] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE NLK antibody, antibody diluted at 1:1000.")
![Immunocytochemistry/ Immunofluorescence TR4/NR2C2 Antibody (H0107B) [Unconjugated]](https://images.novusbio.com/images/pp-h0107b-00_human-tr4-nr2c2-mab-clone-h0107b-41202412514818.jpg)
![Western Blot TR4/NR2C2 Antibody (H0107B) [Unconjugated]](https://images.novusbio.com/images/pp-h0107b-00_human-tr4-nr2c2-mab-clone-h0107b-41202412511338.jpg)
![Western Blot JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Western_Blot_5991.jpg)
![Western Blot JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Western_Blot_6327.jpg)
![Immunocytochemistry JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Immunocytochemistry__Immunofluorescence_20184.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")