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CDX2 Antibody

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Western Blot: CDX2 Antibody [NBP2-24583] - Total protein from human HeLa, HepG2, CaCo2 and HCT116, mouse 3T3 and Neuro2A and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CDX2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.
Western Blot: CDX2 Antibody [NBP2-24583] - Total protein from human HeLa, HepG2, CaCo2 and HCT116, mouse 3T3 and Neuro2A and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and ...read more
Immunohistochemistry-Paraffin: CDX2 Antibody [NBP2-24583] - Formalin-fixed, paraffin-embedded human spleen probed with CDX2 / CDX3 antibody at 5 ug/ml.
Western Blot: CDX2 Antibody [NBP2-24583] - Analysis of CDX3/CDX2 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Eq, Ma-Op, Pm, Pm, RMSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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CDX2 Antibody Summary

Immunogen
A portion of amino acids 1-60 of human CDX2 /CDX3 was used as the immunogen for the antibody.
Predicted Species
Monkey (93%), Rhesus Macaque (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDX2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-3 ug/ml

Reactivity Notes

The amino acid sequence used as immunogen for the GATA-4 antibody is 93% homologous in Rhesus Macaque, 87% homologous in Rat, 82% in Mouse, and 81% homologous in Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDX2 Antibody

  • caudal type homeo box transcription factor 2
  • caudal type homeobox 2
  • Caudal-type homeobox protein 2
  • CDX2
  • CDX3
  • CDX-3
  • CDX3caudal type homeobox transcription factor 2
  • homeobox protein CDX-2

Background

The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CDX2
Uniprot