![Western Blot: SAP130 Antibody (S07-2K8) [NBP3-19974] - Western blot detection of SAP130 in SHSY-5Y, C6 cell lysates using NBP3-19974 (1:1000 diluted). Predicted band size: 136KDa. Observed band size: 136KDa.](http://images.novusbio.com/fullsize/SAP130-Antibody-S07-2K8-Western-Blot-NBP3-19974-img0002.jpg "Western Blot: SAP130 Antibody (S07-2K8) [NBP3-19974] - Western blot detection of SAP130 in SHSY-5Y, C6 cell lysates using NBP3-19974 (1:1000 diluted). Predicted band size: 136KDa. Observed band size: 136KDa.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | S07-2K8 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.3 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide of human SAP130 (Uniprot # Q15393) |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SF3B3 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 136 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA |
| Preservative | 0.01% Sodium Azide |
| Concentration | 0.3 mg/ml |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SF3B3 |
![Immunocytochemistry/Immunofluorescence: SAP130 Antibody (S07-2K8) [NBP3-19974] - Immunocytochemistry of SAP130 (green) in Hela cells using NBP3-19974 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/SAP130-Antibody-S07-2K8-Immunocytochemistry-Immunofluorescence-NBP3-19974-img0001.jpg "Immunocytochemistry/Immunofluorescence: SAP130 Antibody (S07-2K8) [NBP3-19974] - Immunocytochemistry of SAP130 (green) in Hela cells using NBP3-19974 at dilution 1/50, and DAPI (blue)")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")