![Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - Hepatic mincle (CLEC4E) in cirrhosis and ACLF. Representative Western blot showing protein levels of mincle in control, cirrhotic induced by bile-duct ligation (BDL), and rats with acute-on-chronic liver failure (ACLF) induced by lipopolysaccharide (LPS) within cirrhosis and quantification of the Western blots. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fimmu.2020.01352/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Western-Blot-NBP1-49311-img0022.jpg "Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - Hepatic mincle (CLEC4E) in cirrhosis and ACLF. Representative Western blot showing protein levels of mincle in control, cirrhotic induced by bile-duct ligation (BDL), and rats with acute-on-chronic liver failure (ACLF) induced by lipopolysaccharide (LPS) within cirrhosis and quantification of the Western blots. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fimmu.2020.01352/full) licensed under a CC-BY license.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | 16E3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant human CLEC4E (41-219aa) purified from E. coli (NP_055173). |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CLEC4E |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 25 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 10% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: CLEC4E Antibody (16E3) [NBP1-49311] - HeLa cells stained with monoclonal anti-human MINCLE antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Immunocytochemistry-Immunofluorescence-NBP1-49311-img0011.jpg "Immunocytochemistry/Immunofluorescence: CLEC4E Antibody (16E3) [NBP1-49311] - HeLa cells stained with monoclonal anti-human MINCLE antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).")
![Immunohistochemistry-Paraffin: CLEC4E Antibody (16E3) [NBP1-49311] - Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human MINCLE antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Immunohistochemistry-Paraffin-NBP1-49311-img0010.jpg "Immunohistochemistry-Paraffin: CLEC4E Antibody (16E3) [NBP1-49311] - Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human MINCLE antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).")
![Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - An intracellular stain was performed on U87MG cells with CLEC4E Antibody (16E3) NBP1-49311AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Flow-Cytometry-NBP1-49311-img0020.jpg "Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - An intracellular stain was performed on U87MG cells with CLEC4E Antibody (16E3) NBP1-49311AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647")
![Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CLEC4E (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Western-Blot-NBP1-49311-img0009.jpg "Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CLEC4E (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - The Cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-human MINCLE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : CLEC4E transfected 293T cell lysate](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Western-Blot-NBP1-49311-img0017.jpg "Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - The Cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-human MINCLE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : CLEC4E transfected 293T cell lysate")
![Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - Mincle (CLEC4E) in NASH. Representative western blot showing protein levels of mincle in NASH patients and non-NASH controls and quantification of the Western blots. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fimmu.2020.01352/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Western-Blot-NBP1-49311-img0021.jpg "Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - Mincle (CLEC4E) in NASH. Representative western blot showing protein levels of mincle in NASH patients and non-NASH controls and quantification of the Western blots. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fimmu.2020.01352/full) licensed under a CC-BY license.")
![Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - Flow cytometry analysis of CLEC4E in LNCap cell line,staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Flow-Cytometry-NBP1-49311-img0016.jpg "Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - Flow cytometry analysis of CLEC4E in LNCap cell line,staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.Isotype control antibody was mouse IgG (black line).")
![Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - An intracellular stain was performed on U-937 cells with CLEC4E Antibody (16E3) NBP1-49311AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Flow-Cytometry-NBP1-49311-img0018.jpg "Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - An intracellular stain was performed on U-937 cells with CLEC4E Antibody (16E3) NBP1-49311AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - An intracellular stain was performed on U87MG cells with CLEC4E Antibody (16E3) NBP1-49311AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CLEC4E-Antibody-16E3-Flow-Cytometry-NBP1-49311-img0019.jpg "Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - An intracellular stain was performed on U87MG cells with CLEC4E Antibody (16E3) NBP1-49311AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
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![Flow Cytometry: CLEC4E Antibody (16E3) [Alexa Fluor® 488] [NBP1-49311AF488] - An intracellular stain was performed on U-937 cells with CLEC4E Antibody (16E3) NBP1-49311AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](https://images.novusbio.com/images/CLEC4E-Antibody-16E3-Alexa-Fluor-R-488-Flow-Cytometry-NBP1-49311AF488-img0001.jpg "Flow Cytometry: CLEC4E Antibody (16E3) [Alexa Fluor® 488] [NBP1-49311AF488] - An intracellular stain was performed on U-937 cells with CLEC4E Antibody (16E3) NBP1-49311AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")