![Western Blot: SF3B4 Antibody (3A1) [NBP1-92692] - Analysis of different cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to splicing factor SF3B4, NBP1-92692, dilution 1:1,000 in green: [1] protein standard (red), [2] NIH-3T3 cytosolic fraction [3] NIH-3T3 nuclear fraction [4] HeLa cytosolic and [5] HeLa nuclear fractions. Strong single band at 49kDa represents the SF3B4 protein, which is expressed exclusively in the nuclei. The same blot was simultaneously probed with rabbit pAb to GAPDH, dilution 1:20,000, in red. The 37kDa band corresponds to GAPDH protein, detected mainly in the cytosolic fractions of these cells.](http://images.novusbio.com/fullsize/SF3B4-Antibody-3A1-Western-Blot-NBP1-92692-img0005.jpg "Western Blot: SF3B4 Antibody (3A1) [NBP1-92692] - Analysis of different cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to splicing factor SF3B4, NBP1-92692, dilution 1:1,000 in green: [1] protein standard (red), [2] NIH-3T3 cytosolic fraction [3] NIH-3T3 nuclear fraction [4] HeLa cytosolic and [5] HeLa nuclear fractions. Strong single band at 49kDa represents the SF3B4 protein, which is expressed exclusively in the nuclei. The same blot was simultaneously probed with rabbit pAb to GAPDH, dilution 1:20,000, in red. The 37kDa band corresponds to GAPDH protein, detected mainly in the cytosolic fractions of these cells.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 3A1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length recombinant human SF3B4 expressed in and purified from E. coli. [UniProt# Q15427] |
| Localization | Nucleus. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SF3B4 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This SF3B4 (3A1) antibody is useful for Immunocytochemistry/Immunofluorescence, immunohistochemistry, and Western blot, where a band can be seen at approximately 49 kDa. |
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| Theoretical MW | 49 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: SF3B4 Antibody (3A1) [NBP1-92692] - Analysis of HeLa cells stained with mouse mAb to splicing factor SF3B4, NBP1-92692, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The NBP1-92692 antibody reveals strong granular staining of the nuclei, while the vimentin antibody specifically labels cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/SF3B4-Antibody-3A1-Immunocytochemistry-Immunofluorescence-NBP1-92692-img0004.jpg "Immunocytochemistry/Immunofluorescence: SF3B4 Antibody (3A1) [NBP1-92692] - Analysis of HeLa cells stained with mouse mAb to splicing factor SF3B4, NBP1-92692, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. The NBP1-92692 antibody reveals strong granular staining of the nuclei, while the vimentin antibody specifically labels cytoplasmic intermediate filaments.")
![Immunocytochemistry/Immunofluorescence: SF3B4 Antibody (3A1) [NBP1-92692] - HeLa cells stained with NBP1-92692 (red), chicken antibody to Vimentin (NB300-223, green) and DNA (blue, stained with DAPI). The SF3B4 (3A1) antibody reveals strong granular nuclear staining which is a little different from the DNA stain and presumably reflects splicosomal complexes. The Vimentin antibody stains the cytoplasmic intermediate filament network of the HeLa cells.](http://images.novusbio.com/fullsize/SF3B4-Antibody-3A1-Immunocytochemistry-Immunofluorescence-NBP1-92692-img0003.jpg "Immunocytochemistry/Immunofluorescence: SF3B4 Antibody (3A1) [NBP1-92692] - HeLa cells stained with NBP1-92692 (red), chicken antibody to Vimentin (NB300-223, green) and DNA (blue, stained with DAPI). The SF3B4 (3A1) antibody reveals strong granular nuclear staining which is a little different from the DNA stain and presumably reflects splicosomal complexes. The Vimentin antibody stains the cytoplasmic intermediate filament network of the HeLa cells.")
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