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KAT2A/GCN5 Antibody

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Western Blot: KAT2A/GCN5 Antibody [NBP2-17017] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GCN5 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: KAT2A/GCN5 Antibody [NBP2-17017] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.
Western Blot: KAT2A/GCN5 Antibody [NBP2-17017] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: KAT2A/GCN5 Antibody [NBP2-17017] - 293T whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GCN5 antibody [C2C3], C-term diluted at 1:1000. The ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

KAT2A/GCN5 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human GCN5. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Rat (100%), Chicken (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KAT2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for KAT2A/GCN5 Antibody

  • EC 2.3.1.48
  • GCN5 general control of amino-acid synthesis 5-like 2 (yeast)
  • GCN5L2
  • GCN5L2GCN5 (general control of amino-acid synthesis, yeast, homolog)-like 2
  • GCN5MGC102791
  • General control of amino acid synthesis protein 5-like 2
  • General control of amino acid synthesis, yeast, homolog-like 2
  • HGCN5
  • Histone acetyltransferase GCN5
  • histone acetyltransferase KAT2A
  • hsGCN5
  • K(lysine) acetyltransferase 2A
  • KAT2A
  • KAT2A/GCN5
  • Lysine acetyltransferase 2A
  • PCAF-B
  • STAF97

Background

KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA (MIM 164014) in a HAT-independent manner (Mao et al., 2009 [PubMed 19339690]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KAT2A
Uniprot