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S100A13 Antibody

Images

 
Western Blot: S100A13 Antibody [NBP1-86039] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: S100A13 Antibody [NBP1-86039] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: S100A13 Antibody [NBP1-86039] - Staining of human thyroid gland shows strong cytoplasmic and nuclear positivity in glandular cells.
Western Blot: S100A13 Antibody [NBP1-86039] - Analysis in human cell line MCF-7.
Western blot analyses of ANXA3, CMBL & S100A13 expression.Depleted cyst fluids from cPTCs & benign lesions are shown together with total protein extract from A549 cells. Protein sizes are indicated to the right in kDa. ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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S100A13 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ELEESIETVVTTFFTFARQEGRKDSLSVNEFKELVTQQLPHLLKDVGSLDEKMKSLDVNQDSELKFNEYWRLIGELAKEIRKKKDLKI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
S100A13
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
IHC, WB reported in scientific literature (PMID: 24722184). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
S100A13 Protein (NBP1-86039PEP)
Publications
Read Publications using
NBP1-86039 in the following applications:

  • 2 publications
  • WB
    2 publications

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24722184). Mouse (82%%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for S100A13 Antibody

  • S100 calcium binding protein A13
  • S100 calcium-binding protein A13protein S100-A13
  • S100A13

Background

S100A13 is encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is widely expressed in various types of tissues with a high expression level in thyroid gland. In smooth muscle cells, this protein co-expresses with other family members in the nucleus and in stress fibers, suggesting diverse functions in signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100A13 Antibody (NBP1-86039)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IF/IHC, WB.


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IF/IHC
(2)
WB
(2)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol S100A13