Have any of your MRP8 antibodies been tested for use in neutralization, or blocking assays, on human samples?

Name: S100A8 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF3059
Rating: 3.5 (2 reviews)

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Unfortunately at this time we have not tested, or received customer feedback on our MMP8 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our MMP8 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	MuSpecies Glossary
Applications	WB, Simple Western, ICC/IF
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Biotin BAF3059

Catalog# & Formulation	Size	Price
Carrier Free AF3059-SP

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF3059

S100A8 Antibody [Unconjugated]

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Publications for S100A8 Antibody (AF3059)(26)

Reviews for S100A8 Antibody (AF3059) (2) 3.52

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Immunogen	E. coli-derived recombinant mouse S100A8 Pro2-Glu89 Accession # P27005
Specificity	Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) S100A9, rmS100A10, and recombinant human S100B is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	S100A8
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Publications using AF3059	Applications	Species
Yang, Y;Abbas, S;Sayem, MA;Dutta, A;Mohi, G; SRSF2 mutation reduces polycythemia and impairs hematopoietic progenitor functions in JAK2V617F-driven myeloproliferative neoplasm Blood cancer journal 2023-11-27 [PMID: 38012156] (Western Blot, Mouse)	Western Blot	Mouse
Wang J, Rattner A, Nathans J Bacterial meningitis in the early postnatal mouse studied at single-cell resolution eLife 2023-06-15 [PMID: 37318981]
Xu XJ, Ge QQ, Yang MS et al. Neutrophil-derived interleukin-17A participates in neuroinflammation induced by traumatic brain injury Neural regeneration research 2023-05-01 [PMID: 36254991]
C Conrad, D Yildiz, SJ Cleary, A Margraf, L Cook, U Schlomann, B Panaretou, JL Bowser, H Karmouty-Q, J Li, NK Berg, SC Martin, A Aljohmani, SF Moussavi-H, KM Wang, JJ Tian, M Magnen, C Valet, L Qiu, JP Singer, HK Eltzschig, CAPSys Stu, W Bertrams, S Herold, N Suttorp, B Schmeck, ZT Ball, A Zarbock, MR Looney, JW Bartsch ADAM8 signaling drives neutrophil migration and ARDS severity JCI Insight, 2022-02-08;7(3):. 2022-02-08 [PMID: 35132956] (IHC, Mouse)	IHC	Mouse
G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin Single cell transcriptomic landscape of diabetic foot ulcers Nature Communications, 2022-01-10;13(1):181. 2022-01-10 [PMID: 35013299] (IHC, Human)	IHC	Human
YT Ho, T Shimbo, E Wijaya, T Kitayama, S Takaki, K Ikegami, K Miyashita, Y Ouchi, E Takaki, R Yamamoto, Y Kaneda, K Tamai Longitudinal single cell transcriptomics reveals a role for Serpina3n-mediated resolution of inflammation in a mouse colitis model Cellular and Molecular Gastroenterology and Hepatology, 2021-04-13;0(0):. 2021-04-13 [PMID: 33862275] (IHC, Mouse)	IHC	Mouse
Darrell Pilling, Tejas R. Karhadkar, Richard H. Gomer A CD209 ligand and a sialidase inhibitor differentially modulate adipose tissue and liver macrophage populations and steatosis in mice on the Methionine and Choline-Deficient (MCD) diet PLOS ONE 2020-12-30 [PMID: 33378413]
MJ Kim, JY Kim, M Kang, MH Won, SH Hong, Y Her Reduced Fecal Calprotectin and Inflammation in a Murine Model of Atopic Dermatitis Following Probiotic Treatment Int J Mol Sci, 2020-05-31;21(11):. 2020-05-31 [PMID: 32486523] (Western Blot, Mouse)	Western Blot	Mouse
S Maekawa, S Onizuka, S Katagiri, M Hatasa, Y Ohsugi, N Sasaki, K Watanabe, A Ohtsu, R Komazaki, K Ogura, T Miyoshi-Ak, T Iwata, H Nitta, Y Izumi RNA sequencing for ligature induced periodontitis in mice revealed important role of S100A8 and S100A9 for periodontal destruction Sci Rep, 2019-10-11;9(1):14663. 2019-10-11 [PMID: 31605018] (IHC-P, Mouse)	IHC-P	Mouse
YE Kim, M Lee, H Gu, J Kim, S Jeong, S Yeo, YJ Lee, SH Im, YC Sung, HJ Kim, IL Weissman, GO Ahn Hypoxia-inducible factor-1 (HIF-1) activation in myeloid cells accelerates DSS-induced colitis progression in mice Dis Model Mech, 2018-07-30;0(0):. 2018-07-30 [PMID: 29967068] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
A Dufour, CL Bellac, U Eckhard, N Solis, T Klein, R Kappelhoff, N Fortelny, P Jobin, J Rozmus, J Mark, P Pavlidis, V Dive, SJ Barbour, CM Overall C-terminal truncation of IFN-? inhibits proinflammatory macrophage responses and is deficient in autoimmune disease Nat Commun, 2018-06-20;9(1):2416. 2018-06-20 [PMID: 29925830] (Western Blot, Human)	Western Blot	Human
Exploring experimental cerebral malaria pathogenesis through the characterisation of host-derived plasma microparticle protein content Sci Rep, 2016-12-05;6(0):37871. 2016-12-05 [PMID: 27917875] (Western Blot, Mouse)	Western Blot	Mouse
Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium Infect Immun, 2016-08-19;0(0):. 2016-08-19 [PMID: 27382022] (Western Blot, Mouse)	Western Blot	Mouse
Heather L. Clark, Anupam Jhingran, Yan Sun, Chairut Vareechon, Steven de Jesus de Jesus Carrion, Eric P. Skaar et al. Zinc and Manganese Chelation by Neutrophil S100A8/A9 (Calprotectin) Limits Extracellular Aspergillus fumigatus Hyphal Growth and Corneal Infection The Journal of Immunology 2016-01-01 [PMID: 26582948]
Thomsen M, Bakiri L, Hasenfuss S, Wu H, Morente M, Wagner E Loss of JUNB/AP-1 promotes invasive prostate cancer. Cell Death Differ, 2014-12-19;22(4):574-82. 2014-12-19 [PMID: 25526087] (Western Blot, Mouse)	Western Blot	Mouse
Yajima-Himuro , Sara, Oshima , Masamits, Yamamoto , Gou, Ogawa , Miho, Furuya , Madoka, Tanaka , Junichi, Nishii , Kousuke, Mishima , Kenji, Tachikawa , Tetsuhik, Tsuji , Takashi, Yamamoto , Matsuo The junctional epithelium originates from the odontogenic epithelium of an erupted tooth. Sci Rep, 2014-05-02;4(0):4867. 2014-05-02 [PMID: 24785116] (IHC, Mouse)	IHC	Mouse
Baluk P, Phillips K, Yao L, Adams A, Nitschke M, McDonald D Neutrophil dependence of vascular remodeling after Mycoplasma infection of mouse airways. Am J Pathol, 2014-04-13;184(6):1877-89. 2014-04-13 [PMID: 24726646] (IHC, Mouse)	IHC	Mouse
Baluk P, Adams A, Phillips K, Feng J, Hong Y, Brown M, McDonald D Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation. Am J Pathol, 2014-03-11;184(5):1577-92. 2014-03-11 [PMID: 24631179] (IHC, Mouse)	IHC	Mouse
Kim H, Chang K, Ha T, Kim J, Ha S, Shin K, Moon C, Nacken W, Kim H, Suh Y S100A9 knockout decreases the memory impairment and neuropathology in crossbreed mice of Tg2576 and S100A9 knockout mice model. PLoS ONE, 2014-02-25;9(2):e88924. 2014-02-25 [PMID: 24586443] (Western Blot, Mouse)	Western Blot	Mouse
Behnsen J, Jellbauer S, Wong C, Edwards R, George M, Ouyang W, Raffatellu M The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria. Immunity, 2014-02-06;40(2):262-73. 2014-02-06 [PMID: 24508234] (Western Blot, Mouse)	Western Blot	Mouse
Yano J, Palmer G, Eberle K, Peters B, Vogl T, McKenzie A, Fidel P Vaginal epithelial cell-derived S100 alarmins induced by Candida albicans via pattern recognition receptor interactions are sufficient but not necessary for the acute neutrophil response during experimental vaginal candidiasis. 2013-12-09;0(0):. 2013-12-09 [PMID: 24478092] (Neutralization, Mouse)	Neutralization	Mouse
Sade-Feldman M, Kanterman J, Ish-Shalom E, Elnekave M, Horwitz E, Baniyash M Tumor necrosis factor-alpha blocks differentiation and enhances suppressive activity of immature myeloid cells during chronic inflammation. Immunity, 2013-03-07;38(3):541-54. 2013-03-07 [PMID: 23477736] (Neutralization, Western Blot, Mouse)	Neutralization, Western Blot	Mouse
Corinne Cayatte, Barbara Joyce-Shaikh, Felix Vega, Katia Boniface, Jeffrey Grein, Erin Murphy et al. Biomarkers of Therapeutic Response in the IL-23 Pathway in Inflammatory Bowel Disease Clinical and Translational Gastroenterology 2012-02-16 [PMID: 23238132]
Yano , Junko, Kolls , Jay K, Happel , Kyle I, Wormley , Floyd, Wozniak , Karen L, Fidel , Paul L J The Acute Neutrophil Response Mediated by S100 Alarmins during Vaginal Candida Infections Is Independent of the Th17-Pathway. PLoS ONE, 2012-09-25;7(9):e46311. 2012-09-25 [PMID: 23050010] (ELISA Development, Mouse)	ELISA Development	Mouse
Gonzalez-Lopez A, Aguirre A, Lopez-Alonso I, Amado L, Astudillo A, Fernandez-Garcia MS, Suarez MF, Batalla-Solis E, Colado E, Albaiceta GM MMP-8 Deficiency Increases TLR/RAGE Ligands S100A8 and S100A9 and Exacerbates Lung Inflammation during Endotoxemia. PLoS ONE, 2012-06-29;7(6):e39940. 2012-06-29 [PMID: 22768176] (Western Blot, Mouse)	Western Blot	Mouse
Cuervo H, Guerrero NA, Carbajosa S, Beschin A, De Baetselier P, Girones N, Fresno M Myeloid-Derived Suppressor Cells Infiltrate the Heart in Acute Trypanosoma cruzi Infection. J. Immunol., 2011-07-29;187(5):2656-65. 2011-07-29 [PMID: 21804013] (Western Blot, Mouse)	Western Blot	Mouse
Show All 26 Publications. Collapse Publications.

	Verified Customer 01/07/2015
Application:	WB
Species:	Mouse

	Verified Customer 01/07/2015
Application:	WB
Species:	Mouse

Gene Symbol	S100A8
Uniprot	P27005()