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FGF acidic/FGF1 Antibody [Unconjugated]

 (12 Publications)
 
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Western blot shows lysates of human brain (hypothalamas) tissue and human heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human FGF acidic/FGF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF232) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FGF acidic/FGF1 at approximately 16-17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human brain (hypothalamas) tissue and human heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human FGF acidic/FGF1 Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more
FGF acidic/FGF1 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human FGF acidic/FGF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF232) at 15 µg/mL overnight at ...read more
Recombinant Human FGF acidic/FGF1 aa 16‑155 (Catalog # 232-FA) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant ...read more
Coculture increases proliferation of me-CH. (a) BrdU was stained for proliferating cells at day 3. Positive cells are shown in red, indicated by white arrowheads. Green cells are PKH67 labeled me-CH. Nuclei were ...read more
Conditioned medium of SSCs increases proliferation of me-CH through FGF1 signaling pathway. (a) BrdU was stained for proliferating cells at day 3 after forming of aggregates. Pellets of me-CH were cultured in SF medium ...read more
Conditioned medium of SSCs increases proliferation of me-CH through FGF1 signaling pathway. (a) BrdU was stained for proliferating cells at day 3 after forming of aggregates. Pellets of me-CH were cultured in SF medium ...read more
Coculture increases proliferation of me-CH. (a) BrdU was stained for proliferating cells at day 3. Positive cells are shown in red, indicated by white arrowheads. Green cells are PKH67 labeled me-CH. Nuclei were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF232
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF232-SP
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FGF acidic/FGF1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human FGF acidic/FGF1
Phe16-Asp155
Accession # P05230
Specificity
Detects human FGF acidic/FGF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant mouse FGF acidic/FGF1 is observed. Neutralizes the biological activity of rhFGF acidic/FGF1. It will also neutralize the biological activity of rh beta -ECGF and bovine FGF acidic/FGF1, although 2‑3 times the amount of IgG is required to neutralize bovine FGF acidic/FGF1 biological activity.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FGF1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization
  • Simple Western 2.5 ug/mL
  • Western Blot 0.25 ug/mL
Publications
Read Publications using
AF232 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF acidic/FGF1 Antibody [Unconjugated]

  • AFGF
  • alpha
  • alpha-ECGF
  • beta-ECGF
  • ECGF
  • ECGFB
  • ECGF-betaAcidic fibroblast growth factor
  • endothelial cell growth factor, beta
  • FGF acidic
  • FGF-1
  • FGFABeta-endothelial cell growth factor
  • FGF-alpha
  • fibroblast growth factor 1 (acidic)
  • GLIO703
  • HBGF1
  • HBGF-1
  • heparin-binding growth factor 1

Background

FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis (1-3). Human FGF acidic shares 54% amino acid sequence identity with FGF basic and 17%‑33% with other human FGFs. It shares 92%, 96%, 96%, and 96% aa sequence identity with bovine, mouse, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. Alternate splicing generates a truncated isoform of human FGF acidic that consists of the N-terminal 40% of the molecule and functions as a receptor antagonist (4). During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1 (5). It is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate (6). The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryogenesis thereby regulate FGF acidic bioactivity (7). The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors (8, 9). Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes (10). Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals (11-13). The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded (14, 15). Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling (16).

  1. Jaye, M. et al. (1986) Science 233:541.
  2. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
  3. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
  4. Yu, Y.L. et al. (1992) J. Exp. Med. 175:1073.
  5. Rajalingam, D. et al. (2007) Biochemistry 46:9225.
  6. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
  7. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
  8. Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274.
  9. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  10. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
  11. Wesche, J. et al. (2006) J. Biol. Chem. 281:11405.
  12. Imamura, T. et al. (1990) Science 249:1567.
  13. Wesche, J. et al. (2005) Biochemistry 44:6071.
  14. Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794.
  15. Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245.
  16. Bouleau, S. et al. (2005) Oncogene 24:7839.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FGF acidic/FGF1
Antibody [Unconjugate
AF232
FGF acidic/FGF1 Antibody [Unconjugated]
Species: Hu
Applications: WB, Simple Western, IHC, B/N

Publications for FGF acidic/FGF1 Antibody (AF232)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC-P, Neutralization, Western Blot.


Filter By Application
IHC-P
(2)
Neutralization
(3)
Western Blot
(4)
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Human
(5)
Mouse
(3)
Rat
(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF232 Applications Species
J Liu, F Zhang, J He, S Wang, L Wang, J Li, W Shi, Y Han, A Gao, Y Sun Tumor-derived Immunoglobulin-like transcript 4 facilitates angiogenesis of colorectal cancer American journal of cancer research, 2023-02-15;13(2):419-435. 2023-02-15 [PMID: 36895964] (Neutralization, Human) Neutralization Human
HA Nicholson, L Sawers, RG Clarke, KJ Hiom, MJ Ferguson, G Smith Fibroblast growth factor signalling influences homologous recombination-mediated DNA damage repair to promote drug resistance in ovarian cancer British Journal of Cancer, 2022-07-01;0(0):. 2022-07-01 [PMID: 35778553] (Western Blot, Human) Western Blot Human
A Lampart, KD Sluzalska, A Czyrek, A Szerszen, J Otlewski, A Wiedlocha, M Zakrzewska Nuclear Localization Sequence of FGF1 Is Not Required for Its Intracellular Anti-Apoptotic Activity in Differentiated Cells Cells, 2022-02-02;11(3):. 2022-02-02 [PMID: 35159330] (Western Blot, Mouse) Western Blot Mouse
Haichuan H, Zofia P, Patricia H et al. Three subtypes of lung cancer fibroblasts define distinct therapeutic paradigms. Cancer Cell. 10/7/2021 [PMID: 34624218]
Sohinee Bhattacharyya, Chet Oon, Aayush Kothari, Wesley Horton, Jason Link, Rosalie C. Sears et al. Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer Journal of Experimental Medicine 8/3/2020 [PMID: 32434218]
M Lakk, V Denes, K Kovacs, O Hideg, BF Szabo, R Gabriel Pituitary Adenylate Cyclase-Activating Peptide (PACAP), a Novel Secretagogue, Regulates Secreted Morphogens in Newborn Rat Retina Invest. Ophthalmol. Vis. Sci, 2017-01-01;58(1):565-572. 2017-01-01 [PMID: 28125843] (Western Blot, Rat) Western Blot Rat
E Delmas, N Jah, C Pirou, S Bouleau, N Le Floch, J-L Vayssière et al. FGF1 C-terminal domain and phosphorylation regulate intracrine FGF1 signaling for its neurotrophic and anti-apoptotic activities Cell Death & Disease 2/4/2016 [PMID: 26844696]
Song , Xuanhe, Xie , Yaoping, Liu , Yang, Shao , Ming, Wang , Wenbo Beneficial effects of coculturing synovial derived mesenchymal stem cells with meniscus fibrochondrocytes are mediated by fibroblast growth factor 1: increased proliferation and collagen synthesis. Stem Cells Int, 2015-03-16;2015(0):926325. 2015-03-16 [PMID: 25852755] (IHC-P, Human) IHC-P Human
King M, Lindberg M, Stodden G, Okuda H, Ebers S, Johnson A, Montag A, Lengyel E, MacLean Ii J, Hayashi K WNT7A/beta-catenin signaling induces FGF1 and influences sensitivity to niclosamide in ovarian cancer. Oncogene, 2014-09-01;34(26):3452-62. 2014-09-01 [PMID: 25174399] (Neutralization, Human) Neutralization Human
Maillard CM, Bouquet C, Petitjean MM, Mestdagt M, Frau E, Jost M, Masset AM, Opolon PH, Beermann F, Abitbol MM, Foidart JM, Perricaudet MJ, Noel AC Reduction of brain metastases in plasminogen activator inhibitor-1-deficient mice with transgenic ocular tumors. Carcinogenesis, 2008-08-27;29(11):2236-42. 2008-08-27 [PMID: 18753414] (Western Blot, Mouse) Western Blot Mouse
Show All 12 Publications.

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Bioinformatics

Gene Symbol FGF1
Uniprot