RPA2 [p Ser33] Antibody

Replication Protein A (RPA) is a single stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 14, 32 and 70kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA is a target for DNA dependent protein kinase (DNA-PK), Ataxia Telangiectasia Mutated gene (ATM) and a cyclin dependent protein kinase. The middle subunit, RPA 32, is phosphorylated during the S-phase of the cell cycle in response to DNA damage, and during apoptosis of Jurkat T-lymphocytes.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: B/N, Block/Neutralize, ICC/IF, Immunohistochemistry, WB, Western Blot.

Showing Publications 1 - 10 of 27. Show All 27 Publications. Collapse Publications.

Publications using NB100-544	Applications	Species
Martinikova AS The role of truncated PPM1D/Wip1 phosphatase in cancer Thesis 2022-01-01 (Western Blot, Human)	Western Blot	Human
Yeo XH, Sundararajan V, Wu Z et al. The effect of inhibition of receptor tyrosine kinase AXL on DNA damage response in ovarian cancer Communications biology 2023-06-22 [PMID: 37349576] (WB, Human)	WB	Human
Rasti G, Becker M, Vazquez BN et al. SIRT1 regulates DNA damage signaling through the PP4 phosphatase complex Nucleic Acids Research 2023-07-21 [PMID: 37309898]
Vaitsiankova A, Burdova K, Sobol M et al. PARP inhibition impedes the maturation of nascent DNA strands during DNA replication Nature Structural & Molecular Biology 2022-04-01 [PMID: 35332322] (Block/Neutralize)	Block/Neutralize
Miyauchi T, Suzuki S, Takeda M et al. Altered replication stress response due to CARD14 mutations promotes recombination-induced revertant mosaicism American journal of human genetics 2021-05-12 [PMID: 34004138]
Venkatesan S, Angelova M, Puttick C et al. Induction of APOBEC3 Exacerbates DNA Replication Stress and Chromosomal Instability in Early Breast and Lung Cancer Evolution Cancer Discovery 2021-10-01 [PMID: 33947663] (Immunohistochemistry)	Immunohistochemistry
Song L, Tang Z, Peng C et al. Cell type-specific genotoxicity in estrogen-exposed ovarian and fallopian epithelium BMC Cancer 2020-10-21 [PMID: 33087072] (ICC/IF, Mouse)	ICC/IF	Mouse
Ma L, Bian X, Lin W The dual HDAC-PI3K inhibitor CUDC-907 displays single-agent activity and synergizes with PARP inhibitor olaparib in Small Cell Lung Cancer J Exp Clin Cancer Res 2020-10-18 [PMID: 33069237]
Elaine Sanij, Katherine M. Hannan, Jiachen Xuan, Shunfei Yan, Jessica E. Ahern, Anna S. Trigos, Natalie Brajanovski, Jinbae Son, Keefe T. Chan, Olga Kondrashova, Elizabeth Lieschke, Matthew J. Wakefield, Daniel Frank, Sarah Ellis, Carleen Cullinane, Jian Kang, Gretchen Poortinga, Purba Nag, Andrew J. Deans, Kum Kum Khanna, Linda Mileshkin, Grant A. McArthur, John Soong, Els M. J. J. Berns, Ross D. Hannan, Clare L. Scott, Karen E. Sheppard, Richard B. Pearson CX-5461 activates the DNA damage response and demonstrates therapeutic efficacy in high-grade serous ovarian cancer Nature Communications 2020-05-26 [PMID: 32457376]
Bartek J, Merchut-Maya JM, Maya-Mendoza A et al. Cancer cell stemness, responses to experimental genotoxic treatments, cytomegalovirus protein expression and DNA replication stress in pediatric medulloblastomas Cell Cycle 2020-02-13 [PMID: 32054408]
Clairmont CS, Sarangi P, Ponnienselvan K et al. TRIP13 regulates DNA repair pathway choice through REV7 conformational change Nat. Cell Biol. 2020-01-01 [PMID: 31915374]
Gustafsson NMS, Farnegardh K, Bonagas N et al. Targeting PFKFB3 radiosensitizes cancer cells and suppresses homologous recombination. Nat Commun 2018-09-24 [PMID: 30250201] (B/N)	B/N
MaYa-Mendoza A, MoudrY P, Merchut-MaYa JM et al. High speed of fork progression induces DNA replication stress and genomic instability. Nature. 2018-07-01 [PMID: 29950726] (WB, Human)	WB	Human
Tang Z, Yang J, Wang X et al. Active DNA end processing in micronuclei of ovarian cancer cells. BMC Cancer 2018-04-16 [PMID: 29661159] (ICC/IF, Human)	ICC/IF	Human
Her J, Ray C, Altshuler J et al. 53BP1 Mediates ATR-Chk1 Signaling and Protects Replication Forks under Conditions of Replication Stress. Mol Cell Biol 2018-04-15 [PMID: 29378830] (WB)	WB
Tanikawa M, Sanjiv K, et al. The spliceosome U2 snRNP factors promote genome stability through distinct mechanisms; transcription of repair factors and R-loop processing. Oncogenesis 2016-12-19 [PMID: 27991914] (WB, Human)	WB	Human
Bass TE, Luzwick JW, Kavanaugh G et al. ETAA1 acts at stalled replication forks to maintain genome integrity. Nat. Cell Biol. 2016-11-01 [PMID: 27723720] (ICC/IF, Human)	ICC/IF	Human
Hasegawa D, Okabe S, Okamoto K et al. G-quadruplex ligand-induced DNA damage response coupled with telomere dysfunction and replication stress in glioma stem cells Biochem. Biophys. Res. Commun. 2016-02-26 [PMID: 26845351] (ICC/IF, Human)	ICC/IF	Human
Moudry P, Watanabe K, Wolanin KM et al. TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity J. Cell Biol. 2016-02-01 [PMID: 26811421] (WB, Human)	WB	Human
Yu ZC, Huang YF, Shieh SY. Requirement for human Mps1/TTK in oxidative DNA damage repair and cell survival through MDM2 phosphorylation. Nucleic Acids Res. 2015-11-03 [PMID: 26531827] (WB, Human)	WB	Human
O Mortusewicz, B Evers, T Helleday. PC4 promotes genome stability and DNA repair through binding of ssDNA at DNA damage sites. Oncogene 2015-05-11 [PMID: 25961912] (ICC/IF, Human)	ICC/IF	Human
Surnameren G, Surnameliu G, Surnameguo G et al. Loss of Smu1 function de-represses DNA replication and over-activates ATR-dependent replication checkpoint. Biochem Biophys Res Commun. 2013-05-29 [PMID: 23727573] (WB, Human)	WB	Human
Feng Z, Zhang J. A dual role of BRCA1 in two distinct homologous recombination mediated repair in response to replication arrest. Nucleic Acids Res 2012-01-01 [PMID: 21954437]
Luo Y, Chen AY, Qiu J et al. Bocavirus infection induces a DNA damage response that facilitates viral DNA replication and mediates cell death. J Virol 2011-01-01 [PMID: 21047968]
Smith KD, Fu MA, Brown EJ et al. Tim-Tipin dysfunction creates an indispensible reliance on the ATR-Chk1 pathway for continued DNA synthesis. J Cell Biol 2009-10-01 [PMID: 19805627]
Feng J, Wakeman T, Yong S et al. Protein phosphatase 2A-dependent dephosphorylation of replication protein A is required for the repair of DNA breaks induced by replication stress. Mol Cell Biol 2009-11-01 [PMID: 19704001]
Lau E, Chiang GG, Abraham RT et al. Divergent S phase checkpoint activation arising from prereplicative complex deficiency controls cell survival. Mol Biol Cell 2009-09-01 [PMID: 19587119]
Show All 27 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	ICC	Human	08/04/2020	View Summary Application Immunocytochemistry Sample Tested L3.6 Pancreatic cancer cell line Species Human Lot A7 Comments Comments Cells were treated with Hydroxyurea 5mM during 2 hours to induce replication stress and RPA32 phosphorylation. RPA32 P-Ser33 primary antibody dilution 1:2500 Secondary antibody: Rabbit Alexa 555 conjugated 1:500 DAPI counterstaining on cell nuclei

Array NB100-544

• RPA2 Antibodies
• RPA2 Lysate
• RPA2 Proteins