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Rit2 Antibody (OTI3F4) - Azide and BSA Free

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Western Blot: Rit2 Antibody (OTI3F4) - Azide and BSA Free [NBP2-73917] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIT2.
Western Blot: Rit2 Antibody (OTI3F4) - Azide and BSA Free [NBP2-73917] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry: Rit2 Antibody (OTI3F4) - Azide and BSA Free [NBP2-73917] - Staining of paraffin-embedded Human lymph node tissue using anti-RIT2 mouse monoclonal antibody.
Flow Cytometry: Rit2 Antibody (OTI3F4) - Azide and BSA Free [NBP2-73917] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RIT2 ...read more
Western Blot: Rit2 Antibody (OTI3F4) - Azide and BSA Free [NBP2-73917] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RIT2 monoclonal antibody.
Immunohistochemistry: Rit2 Antibody (OTI3F4) - Azide and BSA Free [NBP2-73917] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RIT2 mouse monoclonal antibody.
Immunohistochemistry: Rit2 Antibody (OTI3F4) - Azide and BSA Free [NBP2-73917] - Staining of paraffin-embedded Human breast tissue using anti-RIT2 mouse monoclonal antibody.
Immunohistochemistry: Rit2 Antibody (OTI3F4) - Azide and BSA Free [NBP2-73917] - Staining of paraffin-embedded Human pancreas tissue using anti-RIT2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
OTI3F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73917AF350
Alexa Fluor 405
NBP2-73917AF405
Alexa Fluor 488
NBP2-73917AF488
Alexa Fluor 532
NBP2-73917AF532
Alexa Fluor 594
NBP2-73917AF594
Alexa Fluor 647
NBP2-73917AF647
Alexa Fluor 700
NBP2-73917AF700
Alexa Fluor 750
NBP2-73917AF750
Allophycocyanin
NBP2-73917APC
Biotin
NBP2-73917B
DyLight 350
NBP2-73917UV
DyLight 405
NBP2-73917V
DyLight 488
NBP2-73917G
DyLight 550
NBP2-73917R
DyLight 594
NBP2-73917DL594
DyLight 650
NBP2-73917C
DyLight 680
NBP2-73917FR
DyLight 755
NBP2-73917IR
FITC
NBP2-73917F
HRP
NBP2-73917H
Janelia Fluor 549
NBP2-73917JF549
Janelia Fluor 646
NBP2-73917JF646
PE
NBP2-73917PE
PerCP
NBP2-73917PCP
Unconjugated
NBP2-03062
mFluor Violet 450 SE
NBP2-73917MFV450
mFluor Violet 500 SE
NBP2-73917MFV500
mFluor Violet 610 SE
NBP2-73917MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-03062
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Rit2 Antibody (OTI3F4) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human RIT2 (NP_002921) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
RIT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:500
Theoretical MW
24.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for Rit2 Antibody (OTI3F4) - Azide and BSA Free

  • GTP-binding protein Rit2
  • GTP-binding protein Roc2
  • Ras-like protein expressed in neurons
  • Ras-like without CAAX 2
  • Ras-like without CAAX protein 2
  • RIBA
  • Ric (Drosophila)-like, expressed in neurons
  • Ric-like, expressed in neurons
  • RIN
  • Rit2
  • ROC2

Background

RIT2 is a gene that codes for a protein which binds and exchanges GTP and GDP, and has two isoforms with lengths of 217 and 153 amino acids, with weights of approximately 25 and 17 kDa, respectively. Current studies are being done on diseases and disorders relating to this gene including neuronitis, ehrlichiosis, cryoglobulinemia, hepatitis, Parkinson's disease, and schizophrenia. RIT2 has been shown to have interactions with CALM1, CALM2, CALM3, RLF, and HRAS in pathways such as the Trk receptor signaling, signaling to NGF, and signaling to ERKs pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Rit2 Antibody
(OTI3F4) - Azide and ...
NBP2-73917
Rit2 Antibody (OTI3F4) - Azide and BSA Free
Species: Hu, Mu, Rt, Ca, Pm, Pm
Applications: WB, Flow, IHC, CyTOF-ready

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Bioinformatics

Gene Symbol RIT2