![Western Blot: RIT2 Antibody (3F4) [NBP2-03062] Analysis of extracts (35ug) from 9 different cell lines by using anti-RIT2 monoclonal antibody.](http://images.novusbio.com/fullsize/RIT2-Antibody-3F4-Western-Blot-NBP2-03062-img0007.jpg "Western Blot: RIT2 Antibody (3F4) [NBP2-03062] Analysis of extracts (35ug) from 9 different cell lines by using anti-RIT2 monoclonal antibody.")
| Reactivity | Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clone | OTI3F4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.69 mg/ml |
| Immunogen | Full length human recombinant protein of human RIT2 (NP_002921) produced in HEK293T cell. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RIT2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 24.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.69 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: RIT2 Antibody (3F4) [NBP2-03062] - Staining of paraffin-embedded Human pancreas tissue using anti-RIT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RIT2-Antibody-3F4-Immunohistochemistry-Paraffin-NBP2-03062-img0006.jpg "Immunohistochemistry-Paraffin: RIT2 Antibody (3F4) [NBP2-03062] - Staining of paraffin-embedded Human pancreas tissue using anti-RIT2 mouse monoclonal antibody.")
![Flow Cytometry: RIT2 Antibody (3F4) [NBP2-03062] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RIT2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/RIT2-Antibody-3F4-Flow-Cytometry-NBP2-03062-img0001.jpg "Flow Cytometry: RIT2 Antibody (3F4) [NBP2-03062] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RIT2 antibody, and then analyzed by flow cytometry.")
![Western Blot: RIT2 Antibody (3F4) [NBP2-03062] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIT2.](http://images.novusbio.com/fullsize/RIT2-Antibody-3F4-Western-Blot-NBP2-03062-img0002.jpg "Western Blot: RIT2 Antibody (3F4) [NBP2-03062] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIT2.")
![Immunohistochemistry-Paraffin: RIT2 Antibody (3F4) [NBP2-03062] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RIT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RIT2-Antibody-3F4-Immunohistochemistry-Paraffin-NBP2-03062-img0003.jpg "Immunohistochemistry-Paraffin: RIT2 Antibody (3F4) [NBP2-03062] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RIT2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: RIT2 Antibody (3F4) [NBP2-03062] - Staining of paraffin-embedded Human breast tissue using anti-RIT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RIT2-Antibody-3F4-Immunohistochemistry-Paraffin-NBP2-03062-img0004.jpg "Immunohistochemistry-Paraffin: RIT2 Antibody (3F4) [NBP2-03062] - Staining of paraffin-embedded Human breast tissue using anti-RIT2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: RIT2 Antibody (3F4) [NBP2-03062] - Staining of paraffin-embedded Human lymph node tissue using anti-RIT2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RIT2-Antibody-3F4-Immunohistochemistry-Paraffin-NBP2-03062-img0005.jpg "Immunohistochemistry-Paraffin: RIT2 Antibody (3F4) [NBP2-03062] - Staining of paraffin-embedded Human lymph node tissue using anti-RIT2 mouse monoclonal antibody.")
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