Novus Biologicals products are now on bio-techne.com

Human Resistin ELISA Kit (Colorimetric)

Images

 
ELISA: Resistin ELISA Kit [NBP1-83744] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution
Standard Curve Range
31 - 2,000 pg/mL
Sensitivity
3.1 pg/mL

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Human Resistin ELISA Kit (Colorimetric) Summary

Description
This kit is an enzyme-linked immunosorbent assay for quantitative detection.
Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human Resistin positive serum. There was no crossreactivity detected.
Standard Curve Range
31 - 2,000 pg/mL
Sensitivity
3.1 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 8.1%
Intra-Assay
CV% < 5.1%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
RETN

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human Resistin
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Biotin-Conjugate anti-human Resistin polyclonal antibody
  5. Human Resistin Standard lyophilized, 4000 pg/ml upon reconstitution
  6. Stop Solution (1M Phosphoric acid)
  7. Streptavidin-HRP
  8. Substrate Solution (tetramethyl-benzidine)
  9. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human Resistin ELISA Kit (Colorimetric)

  • Adipose tissue-specific secretory factor
  • ADSF
  • ADSFMGC126609
  • C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1
  • C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein
  • Cysteine-rich secreted protein A12-alpha-like 2
  • Cysteine-rich secreted protein FIZZ3
  • FIZZ3
  • FIZZ3MGC126603
  • found in inflammatory zone 3
  • HXCP1
  • Resistin
  • RETN
  • RETN1
  • RSTNXCP1

Background

Resistin is a hormone secreted by adipose tissue. The length of the resistin pre-peptide in human is 108 aminoacids; the molecular weight is ~12.5 kDa. Resistin was originally found to be produced and released from adipose tissue to serve endocrine functions likely involved in insulin resistance. This idea primarily stems from studies demonstrating that serum resistin levels increase with obesity in several model systems. Since these observations, further research has linked resistin to other physiological systems such as inflammation and energy homeostasis. Current research is proposing to link resistin to inflammation and energy homeostasis, including its alleged role in insulin resistance in obese subjects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

DRP300
Species: Hu
Applications: ELISA
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
DLP00
Species: Hu
Applications: ELISA
M6000B
Species: Mu
Applications: ELISA
DY1707
Species: Hu
Applications: ELISA
NB100-594
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
AF2730
Species: Mu
Applications: WB
NBP2-22106
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
AF5739
Species: Hu, Mu, Rt
Applications: WB
NBP2-29355
Species: Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
DCP00
Species: Hu
Applications: ELISA
NBP1-19773
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
MAB8200
Species: Hu, Mu
Applications: IHC
NB600-930
Species: Hu, Rt
Applications: ELISA, WB
NBP1-49533
Species: Hu, Mu, Pl, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, KD, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP1-30027
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for Resistin ELISA Kit (NBP1-83744) (0)

There are no publications for Resistin ELISA Kit (NBP1-83744).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Resistin ELISA Kit (NBP1-83744) (0)

There are no reviews for Resistin ELISA Kit (NBP1-83744). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Resistin ELISA Kit (NBP1-83744) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Resistin Products

Research Areas for Resistin ELISA Kit (NBP1-83744)

Find related products by research area.

Blogs on Resistin.

"Get the skinny" on Adiponectin & Leptin Antibodies
We at Novus Biologicals have an extensive antibody catalog covering lipid and metabolism research, including Adiponectin and leptin antibody products.Excess body fat is linked to many human diseases, including IRS (insulin resistance syndrome), type...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Be the first to review our Human Resistin ELISA Kit (Colorimetric) and receive a gift card or discount.

Bioinformatics

Gene Symbol RETN